Porous sponge umbrella-shaped structure PDMS chip doped with fluorescent dye and preparation method and application of porous sponge umbrella-shaped structure PDMS chip

A technology of fluorescent dyes and porous sponges, applied in fluorescence/phosphorescence, material analysis through optical means, material analysis, etc., can solve the problems of low sensitivity, high detection cost, and narrow application fields, and achieve excellent flexibility and good adsorption sexual effect

Inactive Publication Date: 2019-11-05
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a porous sponge umbrella-shaped PDMS chip doped with fluorescent dyes and its preparation method and application, so as to solve the problem of low sensitivity of heavy metal detection methods in the prior art, complex detection instruments, narrow application fields and difficult detection. high cost problem

Method used

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  • Porous sponge umbrella-shaped structure PDMS chip doped with fluorescent dye and preparation method and application of porous sponge umbrella-shaped structure PDMS chip
  • Porous sponge umbrella-shaped structure PDMS chip doped with fluorescent dye and preparation method and application of porous sponge umbrella-shaped structure PDMS chip
  • Porous sponge umbrella-shaped structure PDMS chip doped with fluorescent dye and preparation method and application of porous sponge umbrella-shaped structure PDMS chip

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the preparation of the porous sponge umbrella structure PDMS chip doped with fluorescent dye

[0044] combine Figure 1-Figure 5 As shown, the preparation of the porous sponge umbrella structure PDMS chip doped with fluorescent dyes includes the following steps:

[0045] 1) Add SU8 epoxy glue to a petri dish with a diameter of 8.5 cm and a thickness of 0.8 cm, and cure it at room temperature for 12 hours to prepare a mold 10 of a certain size; use an engraving machine to carve the cured template of SU8 (4:1) into A certain mold pattern is produced, and the mold pattern includes a sample-introduction area groove 11 arranged in the center, and several connecting channel grooves 12 extending from the sample-introduction area groove 11, and connected to each connecting channel groove 12 end The detection area groove 13, the distance between each detection area groove 13 and the sampling area groove 11 is equal, as figure 2 shown;

[0046] 2) Adding xanthen...

Embodiment 2

[0054] Embodiment 2, the preparation of xanthene fluorescent dye

[0055] According to a preferred embodiment of the present invention, the xanthene fluorescent dye molecular probe is 3-hydroxy-6-diethylamino-9-oxospiroisobenzofuran-xanthene, and the preparation steps are as follows:

[0056] 1) Take a 100ml round bottom flask and add methanesulfonic acid (5ml), benzoic acid (2-(4-diethylamino-2-hydroxybenzoyl)) (313mg, 1ml) and resorcinol (110mg, 1mmol), Heat in an oil bath at 90°C for 24 hours under magnetic stirring;

[0057] 2) Cool the above reaction solution to room temperature, pour it into a 500ml beaker, add 100ml of distilled water, add the prepared 1.0M NaOH solution to adjust the solution to neutrality, and mix the above neutral solution with dichloromethane and acetic acid The ethyl ester extractant was extracted several times. Collect the organic phase of dichloromethane and ethyl acetate, use anhydrous magnesium sulfate to remove water and dry, spin off the so...

Embodiment 3

[0059] Embodiment 3, the preparation of xanthene dye hydrazine hydrate derivative

[0060] Proceed as follows:

[0061] 1) The product 3-hydroxyl-6-diethylamino-9-oxospiroisobenzofuran-xanthene (186mg, 0.6mmol) obtained in the above Example 2, 80% hydrazine hydrate (1.5ml) and no Water ethanol (20ml) was added in a 100ml round bottom flask, and heated under reflux in an oil bath at 95°C for 24 hours;

[0062] 2) the above reaction solution is cooled to room temperature, poured into a 500ml beaker, add 100ml of distilled water and stir evenly, use dichloromethane and ethyl acetate solvents to carry out multiple extractions, collect dichloromethane and ethyl acetate The organic phase was dehydrated and dried with anhydrous magnesium sulfate, and the solvent was spun off by vacuum distillation and rotary evaporation to obtain a crude product, which was purified by silica gel chromatography, and the eluent was dichloromethane and methanol (100:1) . The target product xanthene d...

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Abstract

The invention provides a porous sponge umbrella-shaped structure PDMS chip doped with fluorescent dye and a preparation method and application of the porous sponge umbrella-shaped structure PDMS chip.The preparation method includes the following steps that SU8 epoxy glue is cured under a room temperature, and a certain mold figure is carved; sodium chloride particles with the adhering fluorescentdye are added into test area grooves of a mold, so that the sodium chloride particles are fully absorbed in all the test area grooves; and PDMS and a curing agent are stirred evenly, then added intothe mold, heated for curing, and demoulded; the sodium chloride particles are placed in water to be dissolved in water and dried to obtain a porous sponge umbrella-shaped structure PDMS chip body; andthe body and a PDMS substrate are subjected to plasma bonding to obtain the porous sponge umbrella-shaped structure PDMS chip. According to the porous sponge umbrella-shaped structure PDMS chip, thecost is low, chip design is simple and convenient, dye stability is good, and detection of target objects in an aqueous solution can be achieved, so that the practical application and promotion of thechip are more convenient, and a platform is provided for fluorescent chips used for detection of heavy metals and environmental pollutants is provided.

Description

technical field [0001] The invention belongs to the field of material preparation and chemical analysis, and more specifically relates to a porous sponge umbrella-shaped PDMS chip doped with fluorescent dyes, a preparation method and application thereof. Background technique [0002] With the rapid development of my country's economy and the continuous improvement of industrial technology, a large amount of industrial water is discharged into rivers and oceans, which has brought great pollution to the water environment. The water environment in many areas is facing the problem of heavy metal pollution. After the water polluted by heavy metals is consumed by humans, it will cause various diseases, damage the nervous system, and endanger human health. Therefore, higher requirements are put forward for the detection of heavy metals. [0003] In order to better detect heavy metal ions quickly and conveniently, new technologies need to be developed. Traditional heavy metal dete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/26C08J9/36C08L83/04C08K5/405G01N21/64
CPCC08J9/26C08J9/36C08J2201/0446C08J2383/04C08K5/405G01N21/643
Inventor 梁丽娟班业伟赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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