DNA sequence with multi-tag series connection and application of DNA sequence to protein expression and purification system

A sequence and coding sequence technology, applied in recombinant DNA technology, peptides containing affinity tags, peptides containing His tags, etc., can solve the problem of high time and cost, few applications of expression and purification, and no multi-tag tandem elements. And other issues
CN110408636AActive Publication Date: 2019-11-05KANGMA SHANGHAI BIOTECH LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
KANGMA SHANGHAI BIOTECH LTD
Publication Date
2019-11-05

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Abstract

The invention provides a DNA sequence with multi-tag series connection and application of the DNA sequence to a protein expression and purification system and particularly relates to a nucleic acid structure. The nucleic acid structure is provided with a structure from 5' to 3' in the formula I, wherein the structure is Z1-Z2-Z3-Z4-Z5 (I); in the formula, Z1 to Z5 are elements used for constituting the nucleic acid structure; all '-' parts are bond or nucleotide connecting sequences; Z1 is the coding sequence of leading peptide; Z2 is the coding sequence without ubiquitin or with ubiquitin; Z3is the coding sequence of tab proteins; Z4 is the connecting sequence; and Z5 is the coding sequence without foreign proteins or with the foreign proteins. According to the nucleic acid structure, the efficiency of the foreign protein synthesis can be significantly improved, and the expression and purification processes of the target foreign proteins are simplified.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a DNA sequence with multiple tags in series and its application in a protein expression and purification system. Background technique

[0002] Proteins are important molecules in cells and are involved in almost all functions of cells. Different sequences and structures of proteins determine their different functions [1]. In cells, proteins can be used as enzymes to catalyze various biochemical reactions, and as signal molecules to coordinate various activities of organisms, to support biological forms, store energy, transport molecules, and make organisms move [1]. In the field of biomedicine, protein antibodies, as targeted drugs, are an important means of treating cancer and other diseases[1,2].

[0003] Some translation-enhancing coding sequences and affinity sequences for protein expression and purification have been found in cells from yeast to human, but the research on thes...

Claims

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