Preparation method of mesenchymal stem cells

Abstract

The invention discloses a preparation method of mesenchymal stem cells. The specific manufacturing method comprises the following steps that S1, extracted biological tissue is fully cleaned for standby application; S2, the biological tissue is fed into a centrifugal rotating machine to be rotated at the high speed for separation, and a biological enzyme is added for dissolving; S3, the cells are subjected to induced differentiation; S4, the stem cells are duplicated through a gene cloning method; and S5, a culture medium is manufactured, and the differentiated stem cells are cultured and subjected to refrigerated preservation. The mesenchymal stem cells are manufactured on the basis of adopting an induced differentiation technology and a cloning technology, the obtained stem cells have thehigh purity and the high survival rate and are free of pollution, overall raw materials are wide, the overall manufacturing method is efficient, long-term preservation can be conducted through the manufactured culture medium, and the specific manufacturing method is worth popularizing.

Description

technical field [0001] The invention relates to the technical field of mesenchymal stem cells, in particular to a preparation method of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells were first discovered in the bone marrow, and later found in many kinds of tissues in the process of human development and development. At present, we can isolate and prepare mesenchymal stem cells from bone marrow, fat, synovium, bone, muscle, lung, liver, pancreas and other tissues, as well as amniotic fluid and umbilical cord blood. The most widely used are bone marrow-derived mesenchymal stem cells. However, bone marrow-derived mesenchymal stem cells have the following problems: with aging, the number of stem cells decreases significantly, and the ability to proliferate and differentiate significantly declines; the preparation process is not easy to control the quality; transplantation to the allogeneic body may cause immune reactions; the material is harmful to...

AI Technical Summary

Problems solved by technology

However, bone marrow-derived mesenchymal stem cells have the following problems: with aging, the number of stem cells decreases significantly, and the ability to proliferate and differentiate significantly declines; the preparation process is not easy to control the quality; transplantation to the allogeneic body may cause immune reactions; and when the material is obtained, it is harmful to the patient. Injury, when the patient has bone marrow disease, it cannot be collected, even if it is a healthy donor, too much bone marrow cannot be extracted

[0004] However, the application process of autologous mesenchymal stem cells has gradually exposed some inconveniences: for example, the expansion ability varies greatly among individuals; the potential risk of tumor cell contamination; it takes a certain amount of time to cultivate and cannot adapt to the needs of the disease in time, etc.

These restrict the use of autologous mesenchymal stem cells