A kind of biosynthetic method of 2-hydroxy-3-methyl-3-formyl butyric acid

A formyl butyric acid, methyl technology, applied in the field of biosynthesis of 2-hydroxy-3-methyl-3-formyl butyric acid

Active Publication Date: 2021-09-03
JING JING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the research tends to use aldolase to catalyze the aldol condensation, but the specific condensation reactions catalyzed by aldolase from different sources are also quite different. At present, there is no aldol condensation that catalyzes the aldol condensation of isobutyraldehyde and glyoxylic acid. Enzymes related reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The composite microorganism of this example is composed of Pseudoxanthomonas CICC NO.22101, Lactobacillus plantarum CGMCC NO.19087 and Bacillus subtilis CICC NO.24434, and the ratio of the number of viable bacteria of the above three strains is 1:1:0.2. Adjust the pH of 650g / L (8.8mol) glyoxylic acid aqueous solution 1L to 7.5 with sodium carbonate solution, raise the temperature to 37-40°C, add 10g of complex microorganisms, stir well, slowly add 639g (8.9mol) of isobutyraldehyde dropwise , the process control material pH between 1.5-2.0, add sodium carbonate aqueous solution when the pH drops, add 10% dilute sulfuric acid when the pH rises, the isobutyraldehyde is added dropwise within 8 hours, then react for 2 hours, and the reaction ends After filtering to remove the bacteria, the content of 2-hydroxy-3-methyl-3-formylbutyric acid in the filtrate was 985g, and the yield was 76.7%.

Embodiment 2

[0018] The composite microorganism in this example is composed of Pseudoxanthomonas CICC NO.22101, Lactobacillus plantarum CGMCC NO.19087 and Bacillus subtilis CICC NO.24434, and the ratio of the number of viable bacteria of the above three strains is 1:0.5:0.1. Adjust the pH of 1L of 50g / L (0.68mol) glyoxylic acid aqueous solution to 7.5 with sodium carbonate solution, raise the temperature to 30-33°C, add 8g of complex microorganisms, stir well, and slowly add 49.5g (0.69mol) of isobutyraldehyde dropwise. ), the process control material pH is between 7.5-8.5, add sodium carbonate aqueous solution when pH decreases, add 10% dilute sulfuric acid when pH increases, isobutyraldehyde is added dropwise in 5h, then reacts 1h, reacts After the end, the bacterial cells were removed by filtration, and the content of 2-hydroxy-3-methyl-3-formylbutyric acid in the filtrate was 74.5 g, and the yield was 75.0%.

Embodiment 3

[0020] The composite microorganism in this example is composed of Pseudoxanthomonas CICC NO.22101, Lactobacillus plantarum CGMCC NO.19087 and Bacillus subtilis CICC NO.24434. The ratio of the number of viable bacteria of the above three strains is 1:1:0.1. Adjust 1L of 390g / L (5.3mol) glyoxylic acid aqueous solution to pH 6.5 with sodium carbonate solution, raise the temperature to 37-40°C, add 15g of complex microorganisms, stir well, and slowly add 383.5g (5.3mol) of isobutyraldehyde dropwise ), the process control material pH is between 6.5-7.0, add sodium carbonate aqueous solution when pH decreases, add 10% dilute sulfuric acid when pH increases, isobutyraldehyde drops in 8h and finish, then react 1h, react After the end, the bacteria were removed by filtration, and the content of 2-hydroxy-3-methyl-3-formylbutyric acid in the filtrate was 626g, and the yield was 80.9%.

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PUM

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Abstract

The invention relates to the technical field of biotransformation, and specifically discloses a biosynthesis method of 2-hydroxy-3-methyl-3-formyl butyric acid, which uses glyoxylic acid and isobutyraldehyde as raw materials to produce aldolase Under the catalysis of compound microorganisms, react to produce 2-hydroxy-3-methyl-3-formyl butyric acid, and the compound microorganisms are complex bacteria of Pseudoxanthomonas, Lactobacillus plantarum and Bacillus subtilis. The method of the invention has high selectivity to the aldol condensation reaction of glyoxylic acid and isobutyraldehyde, and the yield of 2-hydroxy-3-methyl-3-formyl butyric acid can reach more than 75%.

Description

technical field [0001] The invention relates to the field of biotransformation, in particular to a biosynthesis method of 2-hydroxy-3-methyl-3-formyl butyric acid. Background technique [0002] 2-Hydroxy-3-methyl-3-formylbutyric acid is an important pharmaceutical and chemical raw material, a key intermediate for the preparation of ketopantoate lactone, and can also be used as a raw material for the synthesis of pantothenic acid and calcium pantothenate . At present, 2-hydroxy-3-methyl-3-formylbutyric acid is mainly prepared by aldol condensation of glyoxylic acid and isobutyraldehyde under the catalysis of organic base. This method reacts under alkaline conditions, which is prone to side reactions such as aldehyde condensation, and the amount of organic base used in the process is large, which is about 1-8% of the quality of the raw material isobutyraldehyde, which needs to be recovered in the post-treatment process. The operation process is cumbersome, the cost is high, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/42C12R1/01C12R1/125C12R1/25
CPCC12P7/42C12P39/00
Inventor 何连顺李斌水
Owner JING JING PHARMA
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