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Detecting method of antibiotics in water body

A detection method, antibiotic technology, applied in the direction of measuring devices, instruments, analysis materials, etc., can solve the problems of long detection cycle, cumbersome pre-treatment, bulky and expensive instruments, etc., and achieve low detection cost, good SERS signal reproducibility, and process Quick and Easy Effects

Inactive Publication Date: 2019-11-08
太原水质监测站有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have certain limitations, such as: the pre-treatment is relatively cumbersome; usually the sample needs to be enriched or concentrated before detection; the required instruments are bulky and expensive, poor portability, long detection cycle, etc. Rapid and sensitive detection requirements for residues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The detection method of antibiotic in water body, comprises the steps:

[0022] S1. Dissolving sulfonamide antibiotics in 10 times the volume fraction of distilled water, adding mesoporous silica and graphene oxide, and mixing evenly to obtain the aqueous phase; the mass ratio of the template molecule to mesoporous silica is 1 : 3, the mass ratio of the template molecule to graphene oxide is 1: 3;

[0023] S2, 2-vinylpyridine, ethylene glycol dimethacrylate, and azobisisobutyronitrile are dissolved in toluene solvent, and the oil phase is obtained by mixing evenly; the mass ratio of the initiator to 2-vinylpyridine It is 1:2, the addition ratio of the ethylene glycol dimethacrylate and azobisisobutyronitrile is 1mL:10mg, and the molar ratio of the sulfa antibiotic to 2-vinylpyridine is 1:3;

[0024] S3. After ultrasonically mixing the water phase prepared in step S1 and the oil phase prepared in step S2, put them in an oven at 60-70°C for polymerization reaction for 90...

Embodiment 2

[0029] The detection method of antibiotic in water body, comprises the steps:

[0030] S1. Dissolving sulfonamide antibiotics in 20 times the volume fraction of distilled water, adding mesoporous silica and graphene oxide, and mixing evenly to obtain the aqueous phase; the mass ratio of the template molecule to mesoporous silica is 1 : 6, the mass ratio of the template molecule to graphene oxide is 1: 5;

[0031] S2, 2-vinylpyridine, ethylene glycol dimethacrylate, and azobisisobutyronitrile are dissolved in toluene solvent, and the oil phase is obtained by mixing evenly; the mass ratio of the initiator to 2-vinylpyridine It is 1:10, the addition ratio of the ethylene glycol dimethacrylate and azobisisobutyronitrile is 1mL:30mg, and the molar ratio of the sulfa antibiotic to 2-vinylpyridine is 1:6;

[0032] S3. After ultrasonically mixing the water phase prepared in step S1 and the oil phase prepared in step S2, put them in an oven at 60-70°C for polymerization reaction for 9...

Embodiment 3

[0037] The detection method of antibiotic in water body, comprises the steps:

[0038] S1. Dissolving sulfonamide antibiotics in 15 times the volume fraction of distilled water, adding mesoporous silica and graphene oxide, and mixing evenly to obtain the aqueous phase; the mass ratio of the template molecule to mesoporous silica is 1 : 4.5, the mass ratio of the template molecule to graphene oxide is 1: 4;

[0039] S2, 2-vinylpyridine, ethylene glycol dimethacrylate, and azobisisobutyronitrile are dissolved in toluene solvent, and the oil phase is obtained by mixing evenly; the mass ratio of the initiator to 2-vinylpyridine It is 1:6, the addition ratio of the ethylene glycol dimethacrylate and azobisisobutyronitrile is 1mL:20mg, and the molar ratio of the sulfa antibiotic to 2-vinylpyridine is 1:4.5;

[0040] S3. After ultrasonically mixing the water phase prepared in step S1 and the oil phase prepared in step S2, put them in an oven at 60-70°C for polymerization reaction fo...

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Abstract

The invention discloses a detecting method of antibiotics in a water body. The method includes the following steps of dissolving a template molecule in distilled water, adding mesoporous silica and graphene oxide to be evenly mixed to obtain a water phase, dissolving a functional monomer, a crosslinking agent and an initiator in a methylbenzene solvent to be evenly mixed to obtain an oil phase, ultrasonically mixing the water phase and the oil phase, putting the mixture into an oven for polymerization reaction to obtain a print polymer, completing the washing and drying treatment of the printpolymer, taking a water sample filtering membrane for removing impurities, adjusting the pH of a water sample passing through the membrane, adding a chelating agent to be mixed, soaking the print polymer without the template molecule in to-be-detected sample liquid for 3-5 minutes, scooping up the print polymer for confocal micro-Raman spectrum detection to obtain a Raman spectrogram, and comparing the obtained Raman spectrogram with a standard Raman spectrogram to judge whether the water sample contains sulfanilamide antibiotics or not and primarily judge the content range of the sulfanilamide antibiotics. The antibiotics in the water body can be rapidly detected at a low cost.

Description

technical field [0001] The invention relates to the detection of antibiotics in water, in particular to a detection method of antibiotics in water. Background technique [0002] Antibiotics are drugs used to prevent and treat infectious diseases. They are widely used in medicine and animal husbandry and play an irreplaceable role. However, due to the widespread use and even abuse of antibiotics in recent years, a large number of original drugs and their derivatives have entered the environment in various ways, inducing organisms in the environment to be sensitive to them, leading to the generation of drug-resistant genes, which seriously threaten human health and Ecological security has attracted widespread attention from all walks of life. Generally, methods for detecting antibiotic residues in the environment include gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography-mass spectrometry (HPLC-MS), and enzyme-linked immunoassay. However, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65C08F226/06C08F222/10C08J9/26
CPCG01N21/658C08F226/06C08J9/26C08J2339/04C08J2201/0424
Inventor 李渊崔晓波曹建明孙瑞高文秀曲文彦周茜张玮梁明明张文芸程曦胡睿娟弓跃华孙晖张泽清茹婕马瑞杰张俊华
Owner 太原水质监测站有限公司
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