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Molecular markers closely linked to male and female spinach and their applications

A molecular marker and spinach technology, applied in the field of molecular genetic breeding, can solve the problems of low accuracy, poor stability, affecting the purification speed of spinach inbred lines and female lines and the process of variety selection, achieving high accuracy, The effect of shortening breeding time and improving breeding efficiency

Active Publication Date: 2022-07-08
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many sex-related molecular markers have been developed at home and abroad, most of these markers are dominant markers with low accuracy and poor stability. Purification speed and variety selection process of inbred and female lines

Method used

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  • Molecular markers closely linked to male and female spinach and their applications
  • Molecular markers closely linked to male and female spinach and their applications
  • Molecular markers closely linked to male and female spinach and their applications

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1 Development of Molecular Markers Closely Linked to Spinach Sex Gene

[0038] 1. Re-sequencing of high multipliers for female and male plants respectively

[0039] The young leaves of 20 female spinach and 20 male spinach were taken, and DNA was extracted by CTAB method. The extracted DNA samples were paired-end resequencing by Baimaike Biotechnology Co., Ltd. using the Illumina Xten platform. The read length of the sequencing sequence was 150 bp and the sequencing depth was 10×.

[0040]2. Female and male plant-specific sequence analysis

[0041] Using bwa (v0.7.17-r1188) software, the sequencing data of 20 female spinach and 20 male spinach were aligned to the reference genome of spinach (http: / / www.spinachbase.org / cgi-bin / spinach / index.cgi). Based on the results of the sequence alignment, the male-specific sequences were screened (Chr4:58765000-58790000) ( figure 1 ). Since the Y chromosome has one more sequence than the X chromosome genome, we used t...

Embodiment 2

[0047] Example 2 SponR used in molecular marker-assisted selection breeding

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Abstract

The invention provides a molecular marker closely linked with the sex of spinach and its application. The molecular marker contains a nucleotide sequence with a polymorphism of T / A located at chromosome 4 of spinach genome at 58764574. The invention develops a molecular marker closely linked with the spinach sex gene based on the KASP technology, and the marker is verified in multiple groups of spinach to prove that it is closely linked with the spinach sex gene and can accurately identify the male and female plants of the spinach. The sex of spinach can be detected by using the specific KASP primer provided by the invention, the analysis throughput is high, the accuracy is high, and it is suitable for the detection of a large number of samples. The co-dominant molecular marker provided by the invention has important practical significance for improving the female line and inbred line of spinach, shortening the breeding time and improving the breeding efficiency.

Description

technical field [0001] The invention belongs to the field of molecular genetics and breeding, in particular to a molecular marker closely linked with the sex of spinach and its application. Background technique [0002] Spinach (Spinacia oleracea L.), also known as Persian grass, red root vegetable, belongs to the genus Spinach of the family Ricaceae, and is a biennial herb with green leaves as the main product organ. Spinach is rich in plant fiber, protein, vitamins (carotene, vitamins B1, B2, B3, E, K, C, etc.), minerals (calcium, phosphorus, iron, potassium, etc.), folic acid and other nutrients. In addition, spinach has the characteristics of strong cold resistance, wide adaptability, short production cycle, high multiple cropping index, and high output value, so it is very popular among Chinese producers and consumers. According to the 2016 FAO statistics (http: / / www.fao.org / faostat / zh / ?#data / QC), my country's spinach output is about 24.47 million tons, accounting for ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6879C12N15/11
CPCC12Q1/6895C12Q1/6879C12Q2600/156
Inventor 刘志远钱伟徐兆生折红兵李国亮张合龙王晓武武剑
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI