High-throughput detection method of multiple ingredients in health care wine

A detection method and technology for health-care wine, which are applied in measurement devices, material separation, and analysis of materials, etc., to achieve the effects of high sensitivity, less interference, and high detection efficiency.

Active Publication Date: 2019-11-15
JING BRAND
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Problems solved by technology

[0004] The purpose of the present invention is to provide a high-throughput detection method for multi-components in health wine to solve the problem that the current Chinese medicine industry uses high-performance liquid chromatography to detect only a single component in medicinal materials. The method utilizes high-performance liquid chromatography and Mass spectrometry can realize simultaneous detection of multiple components, has high sensitivity and specificity, and can make the quality research of health wine with complex components more comprehensive, reliable and effective

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  • High-throughput detection method of multiple ingredients in health care wine
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  • High-throughput detection method of multiple ingredients in health care wine

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Embodiment 1

[0031] A high-throughput detection method for multiple components in health wine of this embodiment comprises the following steps:

[0032] (1) Precisely measure 1.00mL of health wine sample and place it in a 10mL volumetric flask, add 50% methanol solution to it to dilute, make up to the mark, use a vortex mixer to mix evenly, and then filter with a 0.22μm PVDF needle filter to obtain the sample solution to be tested;

[0033] (2) Weigh hydroxysafflor yellow A, ginsenoside Re, and ginsenoside Rg with reference to the weights in Table 1 below 1 , epimedin C, icariin, ginsenoside Rb1, paeonol, eugenol, icariside II and cinnamon aldehyde, and placed in 10 25mL volumetric flasks respectively, adding methanol to dissolve and then constant volume To the scale, shake well to obtain 10 standard solutions respectively, and the concentration of each standard is shown in Table 2 below.

[0034] Table 2

[0035] serial number Compound name Weighing amount (mg) Labeled ...

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Abstract

The invention discloses a high-throughput detection method of multiple ingredients in health care wine. The method comprises steps as follows: (1) a to-be-detected health care wine sample is measured,the sample is pretreated, and a sample solution is obtained; (2) a mixed standard to-be-detected solution is prepared according to ingredients required to be detected; (3) an HPLC (high performance liquid chromatography) separation method is established, and to-be-detected ingredients in the sample are separated through gradient elution of a mobile phase; (4) high-throughput mass spectrometric detection conditions are set, and data acquisition is performed on the sample solution and the mixed standard to-be-detected solution; (5) a standard curve of a standard product of each ingredient is established according to a detection result, and the content of each corresponding ingredient in the sample is calculated according to the standard curve of each ingredient. The detection method performs high-throughput detection on multiple effective ingredients in the health care wine simultaneously, has higher sensitivity and specificity, little interference, is high in detection timeliness and has good application and promotion prospects.

Description

technical field [0001] The invention relates to the technical field of detection and analysis of health wine, in particular to a high-throughput detection method for multiple components in health wine. Background technique [0002] Health wine is a major branch of my country's traditional Chinese medicine industry. It integrates the theory of syndrome differentiation in traditional Chinese medicine, uses wine as a carrier, and endows wine products with health care functions. At present, the functions of health wine mainly include relieving physical fatigue and enhancing immunity, and most of the functional components and symbolic components of health wine are total saponins, crude polysaccharides, total flavonoids, etc. These indicators are mostly the total amount of a large category of functional components , the detection method is mostly determined by color development by ultraviolet spectrophotometry. Although this type of method is fast and simple, it is easily interfer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/72
Inventor 毛琼丽李先芝严玲刘洋
Owner JING BRAND
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