5results about How to "Improve detection timeliness" patented technology

The invention provides a non-stationary industrial process monitoring method and system. The method comprises an offline training stage: calculating a time Laplacian matrix and a space Laplacian matrix based on a historical data matrix; constructing an objective function of the stationary subspace analysis method, and adding a time constraint term of a time Laplacian matrix and a space constraint term of a space Laplacian matrix into the objective function; solving the objective function to obtain a stable projection matrix; calculating a stationary component and a monitoring index of each sample in the data matrix X in sequence; determining a control limit by using a kernel density estimation method; an online monitoring stage: based on the real-time operation data x, calculating a stationary component of the real-time operation data x and a corresponding real-time monitoring index according to the stationary projection matrix, and if the real-time monitoring index is greater than a control limit, judging that the operation of the non-stationary process has a fault; the monitoring accuracy can be improved.

The invention provides a water quality detection system and method, and the system comprises a detection module which comprises a detection electrode and an automatic titration unit, and the detection electrode is configured to detect the concentration of free ammonia in a water sample to be detected and convert the concentration into a potential signal; the automatic titration unit is configured to titrate an alkaline solution into the water sample to be detected so as to convert fixed ammonia into free ammonia; and the control and data processing module is electrically connected with the detection module, and is configured to receive the potential signal, control the start and stop of the automatic titration unit according to the received potential signal, and calculate the total content of free ammonia and the total content of ammonia in the ammonia distillation wastewater according to the potential signal and the titration amount of the alkaline solution. The water quality is monitored in real time, the problems of long detection period and poor time efficiency of the existing ammonia distillation wastewater are solved, manual operation errors are reduced through automatic operation, and the detection precision is improved.

This invention discloses a TaqMan real-time fluorescent PCR detection primer and probe set, kit, and detection method for Cuscuta pentaphyllum. The primer and probe set includes: an upstream primer C. pan F, whose nucleotide sequence is shown in SEQ ID NO.1; a downstream primer C. pan R, whose nucleotide sequence is shown in SEQ ID NO.2; and a TaqMan probe C. pan P, whose nucleotide sequence is shown in SEQ ID NO.3. The primers and probe target specific fragments of the rbcL gene sequence of Cuscuta pentaphyllum, achieving specific recognition of Cuscuta pentaphyllum through sequence complementarity matching. This avoids false positive results caused by cross-reactions with closely related species and host plants at the molecular targeting level, effectively solving the problem of difficulty in distinguishing closely related species in traditional morphological identification. Compared with traditional morphological identification and conventional PCR, this invention also features high detection efficiency, short processing time, simple operation, and wide applicability. It is suitable for precise identification in professional laboratories and can also meet the practical applications in grassroots quarantine and field monitoring scenarios.

The application relates to the technical field of automatic control, in particular to a solid-liquid reaction control device based on digital microfluidics, which comprises a control module, a detection module, a solid fixing module, a solid plate and a digital microfluidic chip; the solid fixing module can be fixed below the digital microfluidic chip; the solid plate is used for loading solid and is detachable, can be inserted into the solid fixing module and can send the solid into the digital microfluidic chip; the digital microfluidic chip is provided with an in-out sample area, a solid-liquid reaction area and a detection area, and the areas are connected through electrodes; the in-out sample area is provided with a liquid storage pool which is used for storing reaction solution; the control module controls the voltage of each electrode to realize micro-droplet movement; the solution in the liquid storage pool is controlled to move to the solid-liquid reaction area, contacts the solid and reacts, and the solution can also be moved out of the solid-liquid reaction area to interrupt the reaction process of the solid; the control module can also control the to-be-detected micro-droplet to move to the detection area; and the detection module detects the to-be-detected micro-droplet in the detection area through a sensor probe thereof.

The application discloses a probe primer group, a kit and a detection method for detecting European dodder, wherein the probe primer group comprises an upstream primer Ce-rbcL-F, a downstream primer Ce-rbcL-R and a TaqMan probe Ce-rbcL-P; the nucleotide sequence of the upstream primer Ce-rbcL-F is shown as SEQ ID NO. 1; the nucleotide sequence of the downstream primer Ce-rbcL-R is shown as SEQ ID NO. 2; and the nucleotide sequence of the TaqMan probe Ce-rbcL-P is shown as SEQ ID NO. 3. The primer probe group is designed based on a specific region of the rbcL gene of European dodder, and specific identification of European dodder can be realized by combining an optimized real-time fluorescent PCR reaction system and conditions, namely, the application has the characteristics of rapidness, strong specificity, high identification accuracy, effectively solves the problem that near relatives are difficult to distinguish in traditional morphological identification, and avoids false positive misjudgment. Compared with traditional morphological identification and conventional PCR, the application also has the characteristics of high detection efficiency, short time consumption, simple operation and wide applicability, and can be applied to not only professional laboratory accurate identification, but also practical application of scenarios such as primary quarantine and field monitoring.