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39results about How to "Improve detection timeliness" patented technology

Downhole rock debris extraction system and control method thereof

The invention discloses a downhole rock debris extraction system and a control method thereof. The control method comprises the steps that a ground control module determines a first corresponding relation between depth information of the well depth and time in real time, and then marker jet information is sent down to a downhole marker module; according to the marker jet information, the downholemarker module sprays markers onto rock debris produced by excavation through an excavation bit; the ground control module determines a second corresponding relation between marker jet time and the types of the markers in real time, a ground detection and extraction module is controlled to carry out marker detection on the extracted rock debris, and the types of the markers are determined; the ground control module determines original depth information corresponding to the rock debris according to detection results, the first corresponding relation and the second corresponding relation; and therequirements of high detection ageing and simple and easy operation for downhole rock debris extraction can be effectively met, and meanwhile, the control method can achieve the purpose of accurate sampling, low sampling cost and high completed amount.
Owner:CNPS PETRO EQUIP CO LTD

Pathogenic microorganism detection system and method based on nanopore sequencing

ActiveCN112967753AHigh error rateHigh sequencing costProteomicsGenomicsVirulenceFungus
The invention discloses a pathogenic microorganism detection system and method based on nanopore sequencing, and belongs to the technical field of microorganism detection. The pathogenic microorganism detection system comprises: a data input module; a database storage module; a pathogenic microorganism identification module; and a result output module. By utilizing the system and the method provided by the invention, pathogens such as bacteria, fungi, viruses, parasites and the like in a sample can be rapidly analyzed. The system and method hav the characteristics of long sequencing reading length, rapid and accurate sequence reading and the like, and can meet the clinical infection POCT detection requirements. Meanwhile, due to the characteristic of long length, the toxicity and drug-resistant genes corresponding to the pathogens can be analyzed through sequencing data, and clinical infection diagnosis and treatment can be effectively assisted.
Owner:美格医学检验所(广州)有限公司

Device and method for detecting blockage degree of submersed nozzle

The invention discloses a device and method for detecting the blockage degree of a submersed nozzle. A supporting base nozzle is placed on a supporting base; afixing piece is connected with the supporting base and used for fixing the nozzle to the supporting base, and an outlet of the nozzle is located above the supporting base; a lens of the infrared camera is arranged opposite to the supportingbaseand is used for shooting a nozzle outlet picture fixed on the supporting base; and apicture analyzer is connected with the infrared camera and is used for carrying out data analysis on pictures shot by the infrared camera. The technical problems that in the prior art, the detection efficiency is low, the blockage degree of a nozzle cannot be detected at the first time after casting is completed, and detection data lag exists are solved. Infrared imaging is matched with picture processing at the first time after casting is completed, the blockage degree of the nozzle can be quantified, nozzle blockage data can be obtained online in real time, and the technical effect of improving the timeliness of nozzleblockage data detection is achieved.
Owner:SHOUGANG CORPORATION

High-throughput detection method of multiple ingredients in health care wine

The invention discloses a high-throughput detection method of multiple ingredients in health care wine. The method comprises steps as follows: (1) a to-be-detected health care wine sample is measured,the sample is pretreated, and a sample solution is obtained; (2) a mixed standard to-be-detected solution is prepared according to ingredients required to be detected; (3) an HPLC (high performance liquid chromatography) separation method is established, and to-be-detected ingredients in the sample are separated through gradient elution of a mobile phase; (4) high-throughput mass spectrometric detection conditions are set, and data acquisition is performed on the sample solution and the mixed standard to-be-detected solution; (5) a standard curve of a standard product of each ingredient is established according to a detection result, and the content of each corresponding ingredient in the sample is calculated according to the standard curve of each ingredient. The detection method performs high-throughput detection on multiple effective ingredients in the health care wine simultaneously, has higher sensitivity and specificity, little interference, is high in detection timeliness and has good application and promotion prospects.
Owner:JING BRAND

Three-dimensional solid-state laser radar detection method and device based on double optical frequency combs

The invention discloses a three-dimensional solid-state laser radar detection method and device based on double optical frequency combs. The method comprises the following steps: two optical frequency combs with different repetition frequencies are modulated by using an intermediate frequency linear sweep frequency signal to obtain two sweep frequency optical frequency comb signals; one optical signal serving as a detection optical signal is sequentially sent to the Rotman optical lens comprising N input ports; the detection optical signals of different input ports are scanned on a plane, and the detection optical signal in each input port is scanned on a theta plane perpendicular to the plane through an optical antenna; the detection light signal is reflected back to the light antenna after encountering a target, coherent detection is realized by the detection light signal and another sweep frequency light frequency comb signal, and target three-dimensional space distribution and speed information can be obtained after signal processing; according to the three-dimensional solid-state laser radar detection device, through a frequency dispersion wave beam scanning technology, a Rotman optical lens wave beam direction control technology and a dual-optical-frequency comb coherent receiving technology, high-precision measurement of target three-dimensional space distribution and speed information can be realized without mechanical scanning.
Owner:ZHEJIANG LAB

Lens detecting system

InactiveCN105783747AReduce labor costsEfficient and Stable DetectionUsing optical meansGratingManipulator
The invention discloses a lens detecting system. The lens detecting system comprises a base on which a to-be-detected area, an unloading area, a manipulator and a detecting device are arranged. The detecting device comprises a sensor and a raster. The manipulator is utilized for automatically acquiring to-be-detected lens in the to-be-detected area and places the to-be-detected lens on the detecting device. The raster of the detecting device detects the outer diameter of the lens. The sensor detects the central thickness and the depth of the lens. After detection, the manipulator moves the lens for placing on the discharging area.
Owner:FOSHAN BEICHUANG PHOTOELECTRIC TECH

Device for rapidly detecting trace water content

InactiveCN103018286ADetection speed shortenedQuick checkMaterial resistanceWater contentDew point
The invention provides a device for rapidly detecting trace water content in an industrial gas. A detection gas passage of the device detects the trace water content in the bottled industrial high-purity gas under the high pressure formed by the bottled gas per se; a resistor-type dew point sensor for transmitting digital signals is adopted in the gas passage; and after detection, the water content is conversed through an instrumental intelligent circuit. The entrance of the detection gas passage is formed by two parallel sets of gas passages which can perform sampling, purging and high-pressure delivery. The two sets of gas passages are controlled by respective switch valves at the entrance of the device to deliver a high-pressure gas sample to the dew point sensor in turn. When one gas passage is in a detection state, the other one is in a purging state, so that the cross-leakage between the two passages of gas samples is avoided. The flow detection switches of the gas passages are mounted behind flow control valves, so that the device is located in the high-pressure gas sample, and when gas to be detected is replaced, the gas samples in the detector and the input pipe thereof are always under the high pressure, so as to enable the balance of data of the detector to be reached within a short time, and the detection is rapid. The detection is both accurate and rapid.
Owner:袁万德

Sweet solution detection method based on cell image recognition

The invention discloses a sweet solution detection method based on cell image recognition. The method comprises the following steps: taking N drops of a sweet solution to be detected, sequentially dropping the sweet solution to be detected onto taste receptor cells for stimulation, and stimulating the taste receptor cells each time to obtain a corresponding measurement signal KC1; and inputting the N measurement signals KC1 as input data KC1 (t) into a layer of nonlinear kinetic model, drawing a signal-to-noise ratio (SNR) curve, finding out the abscissa value of a characteristic peak in the SNR curve, and judging which sweet solution the sweet solution to be detected belongs to according to the abscissa value of the characteristic peak. The cell imaging method is utilized without contacting with the detected cell, the interference brought by the outside is greatly reduced, interference factors influencing the instant physiological state of the cell are eliminated, the detection time is short, and the detection accuracy is high.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Large-scale remote sensing image-based hangar recognition method

The invention relates to the technical field of image recognition, in particular to a large-scale remote sensing image-based hangar recognition method, which comprises the following steps of: S1, carrying out picture preprocessing on a satellite visible light remote sensing image; S2, introducing an attention mechanism to locate an airport position in the remote sensing map; S3, training a YOLOv5 network by using the hangar picture data set; and S4, improving YOLOv5 network detection precision and network training efficiency by using transfer learning and hyper-parameter optimization, and realizing rapid recognition of the hangar from the remote sensing large image. According to the method, the attention mechanism and the deep learning network are combined, the hangar target in the high-precision satellite visible light remote sensing image is recognized step by step, compared with a current universal interpreter searching method, the precision target is guaranteed, meanwhile, the detection timeliness is greatly improved, and certain mobility is provided for recognition of other targets.
Owner:江苏思远集成电路与智能技术研究院有限公司

Copper ion rapid detection method and device

The invention relates to a copper ion rapid detection method and device. The method comprises the following steps: preparing a chromogenic solution; treating a base material; preparing a detector; preparing a sample to be detected; dripping a sample to be detected on the detector, and comparing the color of the detector with the standard colorimetric card to obtain the concentration of the copperions in the sample to be detected. The invention also relates to a rapid copper ion detection device, a detector prepared by adopting the method for rapidly detecting the copper ions and a standard colorimetric card. The method provides convenient conditions for rapid detection of the concentration of copper ions, can semi-quantitatively detect the concentration of milligram-grade copper ions in ato-be-detected sample, can significantly improve the detection timeliness, does not need to prepare a detection solution separately on site, does not pollute the environment, is wide in test range, does not need professional personnel for test operation, and is short in reaction time. The stability is good, other solutions or equipment does not need to be carried, only the detector and the standard colorimetric card need to be carried, and the kit is suitable for field and on-site detection.
Owner:WUHAN UNIV OF TECH

Transformer partial discharge detection method

The invention discloses a transformer partial discharge detection method. Multiple sets of partial discharge detection is performed on the same transformer at different positions by using an ultrasonic transmitting partial discharge detection system, an ultrahigh frequency partial discharge monitoring system and a microprocessor, the detection result can be clearly viewed through analysis and processing of the microprocessor and the two test results verify each other. The advantages of the method are that two sets of partial discharge detection systems are integrated and have complementary advantages, and the two test results verify each other so that the method has high anti-interference performance and high detection timeliness.
Owner:STATE GRID CORP OF CHINA +2

Photoacoustic pathological image processing method, storage medium and terminal equipment

The invention discloses a photoacoustic pathological image processing method, a storage medium and terminal equipment, and the method comprises the steps: respectively obtaining a WSI pathological image and a photoacoustic pathological image of a pathological tissue sample through a digital pathological scanner and a photoacoustic imaging scanner; marking the WSI pathological image, and obtaining a marked WSI pathological image; acquiring a labeled WSI pathological image and a photoacoustic pathological image of the same pathological tissue sample, and labeling the photoacoustic pathological image by adopting a supervised automatic labeling model by taking the labeled WSI pathological image as a reference to obtain a labeled photoacoustic pathological image; and training a generative adversarial network model based on the marked photoacoustic pathological image to realize interaction, association and mutual conversion of the target area in the cross-modal image. According to the invention, benign and malignant, lesion areas, boundaries and other information of pathological tissues can be directly identified from the photoacoustic image.
Owner:SHENZHEN UNIV

Automatic control system and method for ammonia online analyzer

The invention discloses an automatic control system and method for an ammonia online analyzer. The automatic control system comprises a human-computer interaction unit, a data acquisition and automatic control unit, a sampling unit, a flue gas flow control unit, an absorption constant volume unit and a continuous flow analysis unit, wherein the data acquisition and automatic control unit comprisesa data acquisition and processing module and an automatic control module; the data acquisition and processing module is used for transmitting switching value and analog quantity signals of all equipment; and the automatic control module is used for automatically controlling all running equipment in the system according to the written logic and related information input by the human-computer interaction unit. the system integrates sampling, absorption, constant volume and sample feeding capabilities, is high in sampling analysis precision and timeliness, can meet the requirement that the ammonia online analyzer continuously and stably measures the ammonia concentration in the flue gas, and guides the ammonia emission control system to operate.
Owner:BEIJING GUODIAN LONGYUAN ENVIRONMENTAL ENG +1

Power grid open-phase detection device and method for controlling switch tube in rectifier bridge

The invention discloses a power grid open-phase detection device and a method for controlling a switch tube in a rectifier bridge, and the power grid open-phase detection device comprises a voltage detection circuit and an open-phase detection circuit. The input end of the voltage detection circuit is connected with each switch tube in the rectifier bridge, and the voltage detection circuit is used for outputting a first voltage detection signal to the open-phase detection circuit when any switch tube meets a preset condition; when any switch tube does not meet the preset condition, a second voltage detection signal is output to the open-phase detection circuit; the open-phase detection circuit is used for outputting a normal signal when the first voltage detection signal is received; and when the second voltage detection signal is received, an open-phase fault signal is output. The power grid open-phase detection device can solve the technical problems that an existing power grid open-phase fault detection circuit is complex in structure, and time delay is large when power grid open-phase fault detection is carried out in a software mode.
Owner:SUZHOU INOVANCE TECH CO LTD

Glucose solution detection device and method

The invention discloses a glucose solution detection device and method. The device comprises a computer, a cell image acquisition module, a solution conveying module and a cell placement module. The solution conveying module comprises a liquid conveying pump, a plurality of detection containers used for containing to-be-detected solutions and a buffer solution container used for containing pbs buffer solutions. A first infusion tube is arranged at the bottom of the detection container; a first electromagnetic valve is arranged at the front end of the first infusion tube; a second infusion tubeis arranged at the bottom of the buffer solution container, a second electromagnetic valve is arranged at the front end of the second infusion tube, the liquid inlet pipeline of the infusion pump iscommunicated with the first infusion tube and the second infusion tube, the liquid outlet pipeline of the infusion pump is communicated with the cell placement module, and the computer is electricallyconnected with the cell image acquisition module, the infusion pump, the first electromagnetic valve and the second electromagnetic valve. The cell imaging method is utilized without contacting withthe detected cell, interference brought by the outside is reduced, interference factors influencing the instant physiological state of the cell are eliminated and the detection accuracy is high.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Portable animal parasitic disease detection device

PendingCN113671163ASolve the problem of generally low prevention and control effectImprove detection timelinessMaterial analysisAnimal scienceParasitic disease
The invention relates to a portable animal parasitic disease detection device, which comprises a box body, the top of which is provided with a hand-held part; drawable funnel placing racks are embedded in the two sides of the top of the box body. A plurality of storage boxes are arranged in the middle of the box body, and safety locks are mounted on the storage boxes; the universal wheels are arranged at the bottom of the box body. The current animal epidemic disease prevention and control faces the current situation that the parasitic disease prevention and control effect is generally low due to the fact that the timeliness is poor, the sample conveying cost is high and the number of laboratory detection technicians cannot meet the market requirement in the laboratory detection of the parasites in the current animal epidemic disease prevention and control. In order to solve the problems in actual production, the animal parasitic disease detection device is provided, the top of the box body is provided with the hand-held part capable of being lifted, the bottom of the box body is provided with the universal wheels, carrying is convenient, and meanwhile a storage box for storing detection parts and a funnel placement frame are arranged in the box body. The device is directly applied to a detection site, so that the sample detection timeliness can be improved, and the sample conveying cost and the public health risk possibly caused by sample conveying are reduced.
Owner:INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI

An automatic control system and control method for an ammonia on-line analyzer

The invention discloses an automatic control system and control method for an ammonia on-line analyzer, including a human-computer interaction unit, a data acquisition and automatic control unit, a sampling unit, a flue gas flow control unit, an absorption constant volume unit and a continuous flow analysis unit, and a data collection unit. The acquisition and automatic control unit includes a data acquisition and processing module and an automatic control module. The data acquisition and processing module is used to transmit the switching and analog signals of all equipment. The information automatically controls all operating equipment in the system; this system integrates sampling, absorption, constant volume and sample delivery capabilities, and has high sampling and analysis accuracy and timeliness, which can meet the requirements of continuous and stable measurement of flue gas by ammonia on-line analyzers. The ammonia concentration in the system guides the operation of the ammonia emission control system.
Owner:BEIJING GUODIAN LONGYUAN ENVIRONMENTAL ENG +1

A road closure detection method based on multi-feature fusion

The invention discloses a road closure detection method based on multi-feature fusion, which comprises the following steps: in the offline stage, grid processing is performed on the area to be detected and a grid index is established for trajectory data and road network data. Subsequently, the traffic correlation strength between road sections is extracted; at the same time, based on the historical trajectory data, the grid traffic flow sequence, the strongly associated road turning flow sequence of each road section, and the vehicle U-turn frequency sequence in a local area are obtained. In the online detection stage, the combined model of CNN and LSTM is used to predict the current traffic flow of each grid, and to screen candidate closed grids. The combined model of GCN and LSTM is used to predict the turning amount of the strongly associated roads in each road section and the U-turn frequency in a local area, and then the closed road section is identified according to the sudden decrease of the steering flow or the sharp increase of the U-turn frequency. Finally, combined with the driving yaw detection and the bicycle track data, the closed type of the road is further judged. The invention improves detection efficiency to ensure real-time performance.
Owner:EAST CHINA NORMAL UNIV

A sweet solution detection method based on cell image recognition

The invention discloses a sweet solution detection method based on cell image recognition. It includes the following steps: take N drops of the sweet solution to be tested, drop them on the taste receptor cells for stimulation in turn, and stimulate the taste receptor cells each time to obtain a corresponding measurement signal KC1; take N measurement signals KC1 as input data KC1 ( t) Input a layer of nonlinear dynamics model, draw the signal-to-noise ratio SNR curve, find the abscissa value of the characteristic peak in the signal-to-noise ratio SNR curve, and judge which sweet solution to be tested belongs to according to the abscissa value of the characteristic peak a sweet solution. The invention utilizes the cell imaging method without contact with the tested cells, greatly reduces the interference caused by the outside world, eliminates the interference factors affecting the instantaneous physiological state of the cells, and has fast detection time and high detection accuracy.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Bronchiectasis scoring method and system

The invention relates to a bronchiectasis scoring method. According to the bronchiectasia scoring method, a trained Mask-RCNN model is adopted, so that the bronchiectasia position in the lung lobe can be quickly and accurately determined, and the bronchiectasia degree can be accurately scored. Moreover, the lung lobe image is extracted based on the CT image of the patient, so that the accuracy of bronchiectasia position detection and bronchiectasia degree scoring can be improved while the timeliness of bronchiectasia position detection is improved.
Owner:数聚工研(北京)科技有限公司

Test strip card for quantitative detection of classical swine fever virus antibody with double antigen sandwich and double detection line

The invention belongs to the field of animal immune detection, and in particular relates to a test paper card for quantitative detection of swine fever virus antibody with double antigen sandwiches and double detection lines. The test card includes sample pad, classical swine fever virus E2 protein gold standard pad, rabbit IgG gold standard pad, absorbent pad, double detection line coated with classical swine fever virus E2a protein, and nitrocellulose membrane of goat anti-rabbit IgG quality control line and PVC rubber sheet; colloidal gold adopts double-antigen sandwich and double detection line; the classical swine fever virus gold standard pad is coated with the protein antigen secreted by Escherichia coli BL21 / pE2 with the preservation number CCTCC NO: M2018060. E2 antigen and gold-labeled rabbit IgG; the nitrocellulose membrane is coated with the CSFV E2a antigen constructed from the protein antigen secreted by Escherichia coli BL21 / pE2a with the preservation number CCTCC NO: M2018059, constituting a double detection line area and The quality control line region is composed of goat anti-rabbit IgG.
Owner:武汉观锐生物科技有限公司

Rapid quantitative detection method of novel chloramphenicol resistance gene and application thereof

The invention relates to the technical field of environmental and biological detection, in particular to a novel rapid quantitative detection method of a chloramphenicol resistance gene and application thereof. The quantitative detection method comprises the following steps: (1) acquiring and pretreating an environmental sample; (2) extracting genome DNA (deoxyribonucleic acid); (3) performing metagenome sequencing; (4) comparing novel chloramphenicol resistance genes and counting the number of bacteria 16S rRNA genes; and (5) making quantitative analysis of the novel chloramphenicol resistance gene. Compared with the prior art, the invention disclosed by the invention is simple and easy to implement, does not need to construct standard plasmids, is not limited by sample types, is relatively high in detection timeliness, is relatively high in comparison result accuracy, can accurately realize quantitative analysis of the gene in different environmental samples, greatly improves the timeliness of accurate detection. And popularization in actual environmental sample monitoring is facilitated.
Owner:SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV

Ultrasonic phased array system and concrete filled steel tube construction method

The invention discloses an ultrasonic phased array system and a steel tube concrete construction method, and the system comprises an ultrasonic probe array which comprises a plurality of array elements distributed in an array shape; the array element is a dry coupling probe and is used for exciting longitudinal waves as a detection carrier; the magnetic suction device is mounted on the ultrasonic probe array and is used for being adsorbed on the surface of a steel plate of the steel pipe, so that the dry coupling probes are uniformly coupled with the steel plate; the pulse transmitter is used for driving each array element; the signal amplifier is used for amplifying the ultrasonic signals received by the dry coupling probes; and the signal receiving module is used for receiving the ultrasonic signal amplified by the signal amplifier and transmitting the received ultrasonic signal to the data processing module through the wireless transmission module. The system is adsorbed on the surface of a steel plate by adopting the magnet during detection, so that uniform coupling between the probe and a detected medium is realized, a coupling agent does not need to be smeared, the detection speed is high, and the system can find defects in time in the pouring process of the concrete filled steel tube.
Owner:SHANGHAI RES INST OF BUILDING SCI CO LTD

Sweet solution detection device and method

ActiveCN111175298AReflect all-round physiological changesReduce distractionsMaterial analysis by optical meansTaste SenseTaste receptor
The invention discloses a sweet solution detection device and method. The device comprises a computer, a phase difference microscope, infusion equipment and a placing module for placing taste receptorcells. The placing module comprises a slide; a placing groove for placing the taste receptor cells is formed in the upper surface of the slide; a liquid inlet and a liquid outlet are respectively formed in the two sides of the upper surface of the slide; the upper surface of the slide is further provided with a first flow guide groove communicating with the liquid inlet and the containing grooveand a second flow guide groove communicating with the liquid outlet and the containing groove, the liquid inlet is communicated with the nfusion equipment through a pipeline, the liquid outlet is connected with a waste liquid collector through a pipeline, and the phase difference microscope is electrically connected with the computer. The cell imaging method is utilized without contacting with thedetected cell, interference brought by the outside is greatly reduced, interference factors influencing the instant physiological state of the cells are eliminated, the detection time is short and the detection accuracy is high.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Computer equipment, storage medium, state detection method, device and system of servo motor

Embodiments of the present application provide a computer device, a storage medium, and a state detection method, device, and system for a servo motor. The method includes: sampling the feedback voltage output by the servo motor according to the sampling period to obtain the sampling value, and accumulating the quantity of the sampling value to obtain the first set quantity; according to the sampling value, the first set quantity and the sampling period , generate a slope value; judge that the number of accumulated slope values ​​is less than the accumulated threshold or equal to the accumulated threshold; if it is judged that the number of slope values ​​is less than the accumulated threshold, continue to perform the step of sampling the feedback voltage output by the servo motor; When the number of out-of-slope values ​​is equal to the accumulated threshold, it is judged whether the successive accumulated thresholds and the slope values ​​are all smaller than the slope threshold, and the servo motor is controlled by the motor driver chip according to the judgment result. The embodiment of the present application improves the detection timeliness and reduces the detection cost.
Owner:HANGZHOU SANHUA RES INST CO LTD

Steel belt broken wire detection method and system

The invention provides a steel belt broken wire detection method and system. The steel belt broken wire detection method comprises the steps that an impedance electric signal is generated on a steel belt, then the impedance electric signal is obtained from the steel belt, and the steel belt broken wire condition is judged according to the impedance electric signal. The steel belt broken wire detection system comprises an impedance generation device used for generating the impedance electric signal on the steel belt and an impedance detection device which is used for acquiring the impedance electric signal from the steel belt and judging the wire breakage condition of the steel belt according to the impedance electric signal. The steel belt damage information can be accurately fed back in real time based on impedance detection, elevator safety accidents caused by steel belt damage can be effectively avoided, cost is low, debugging is easy, online detection can be achieved, the elevator Internet of Things can be incorporated, the detection timeliness is greatly improved, manpower is liberated, reliable reference is provided for future large-scale use, the system is matched with an elevator operation management system, so that the damage degree of the steel belt can be fed back in real time, and a reliable basis is provided for replacement of the steel belt.
Owner:QINHUANGDAO DEV AREA PROSPECT PHOTOELECTRIC TECH

Primer group, kit and sequencing library for detecting large fragment deletion of rare beta thalassemia gene with unclear fracture site

The invention relates to the field of gene detection, in particular to a primer group, a kit and a sequencing library for detecting large fragment deletion of a rare beta thalassemia gene with an unclear fracture site. A pair of complementary primers which are far away from each other are designed in upstream and downstream regions of a beta-globin gene cluster, a pair of normal internal control complementary primers which are moderate in distance is designed in an HBB gene region, long-fragment PCR amplification is performed, and a third-generation sequencing library is constructed and sequenced; the accurate and rapid detection of the large-fragment deletion variation of the rare beta thalassemia gene with the unclear fracture site is realized. Besides, according to the method, DNA of different sequences of 5-50 nt can be added to the 5'end of the designed primer, namely a DNA barcode (Barcode) for distinguishing different samples and achieving simultaneous detection of multiple samples, and the method is easy and convenient to operate, reliable in result and high in repeatability and has very high clinical applicability and generalizability.
Owner:广西医科大学第二附属医院
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