Application of undecanal in alleviating oxidative stress skin damage
A technology for oxidative stress and skin damage, which is applied in the field of medicine, can solve the problems of low dosage of toxic and side effects, lack of research on active ingredients, and expensive treatment, and achieve cheap prices, good protective effects, and reduced toxic and side effects
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Embodiment 1
[0034] Embodiment 1 uses MTT method to measure the impact of different concentrations of undecanal on the activity of HaCaT cells
[0035] 1. Experimental method
[0036] Digest the HaCaT cells from the culture flask with trypsin, centrifuge for 5 min, add DMEM medium containing 10% FBS and antibiotics (100 units / mL penicillin, 100 μg / mL streptomycin) to make a cell suspension for inoculation Concentration 1×10 4 The concentration of cells / mL was inoculated on a 96-well plate (100 μL / well), placed in 5% CO2, and incubated at 37° C. for 24 hours. Set the concentration of undecanal as 160 μM, 120 μM, 80 μM, 40 μM, 20 μM, 10 μM, 5 μM, and set 6 replicate wells for each concentration. 5%CO 2 After culturing at 37°C for 24 hours, carefully aspirate the culture solution, and add 150 μL of MTT solution (0.5 mg / mL) prepared in serum-free medium to each well; after continuing to cultivate for 4 hours, carefully aspirate the culture solution in the well. Add 150 μL DMSO to each well...
Embodiment 2 10
[0039] Example 2 undecanal to H 2 o 2 Preprotection experiment of induced oxidative stress injury of HaCaT cells
[0040] 1. Experimental method
[0041] Digest the HaCaT cells from the culture flask with trypsin, centrifuge for 5 min, add DMEM medium containing 10% FBS and antibiotics (100 units / mL penicillin, 100 μg / mL streptomycin) to make a cell suspension for inoculation Concentration 1×10 4 The concentration of cells / mL was inoculated in 96-well plates (100 μL / well), placed in 5% CO2, and cultured at 37°C. After 24h, the culture medium was discarded, and the blank group and H 2 o 2The group was added with 100 μL DMEM basal medium, and the experimental group was added with different concentrations of undecanal (160 μM, 120 μM, 80 μM, 40 μM, 20 μM, 10 μM, 5 μM), and three replicate wells were set for each concentration for pretreatment. After 24 hours, add 100 μL of H 2 o 2 (500 μM) DMEM basal medium, and then placed at 37 ° C, 5% CO 2 Cultured in an incubator. A...
Embodiment 3 10
[0044] Example 3 undecanal to H 2 o 2 Preprotection experiment of induced HaCaT cell apoptosis
[0045] 1. Experimental method
[0046] Digest the HaCaT cells from the culture flask with trypsin, centrifuge for 5 min, add DMEM medium containing 10% FBS and antibiotics (100 units / mL penicillin, 100 μg / mL streptomycin) to make a cell suspension for inoculation Concentration 1×10 4 The concentration of cells / mL was inoculated in 96-well plates (100 μL / well), placed in 5% CO2, and cultured at 37°C. After 24h, the culture medium was discarded, and the blank group and H 2 o 2 The group was added with 100 μL DMEM basal medium, and the experimental group was added with different concentrations of undecanal (160 μM, 120 μM, 80 μM, 40 μM, 20 μM, 10 μM, 5 μM), and three replicate wells were set for each concentration for pretreatment. After 24 hours, add 100 μL of H 2 o 2 (500 μM) DMEM basal medium, and then placed at 37 ° C, 5% CO 2 Cultured in an incubator. After treatment, c...
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