A kind of simple and easy-to-manufacture photosynthetic bacteria culture medium and preparation method thereof

A technology of photosynthetic bacteria and culture medium, applied in the biological field, can solve the problems of limiting the application of photosynthetic bacteria, various types of culture medium components, long enrichment time, etc., and achieve simple components and preparation methods, stable sources, and easy-to-obtain raw materials Effect

Active Publication Date: 2020-11-24
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the application advantages of photosynthetic bacteria, it is often necessary to carry out large-scale cultivation of them. However, the medium used for photosynthetic bacteria generally has shortcomings such as long enrichment time, many types of medium components, and high cost, which are extremely limited. The application of photosynthetic bacteria in production

Method used

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  • A kind of simple and easy-to-manufacture photosynthetic bacteria culture medium and preparation method thereof
  • A kind of simple and easy-to-manufacture photosynthetic bacteria culture medium and preparation method thereof
  • A kind of simple and easy-to-manufacture photosynthetic bacteria culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A simple and easy-to-make photosynthetic bacteria culture medium, each liter of culture medium includes the following components:

[0031]

[0032] Make up to 1L with water.

[0033] The preparation method of photosynthetic bacteria culture medium comprises the following steps:

[0034] (1) Add coarse salt and block brown sugar to water and boil together, then remove solid impurities floating on the surface, cool to below 35°C for later use;

[0035] (2) Get nitrogen, phosphorus and potassium compound fertilizer, monosodium glutamate, and multivitamin B, add half the solution of step (1), and mix;

[0036] (3) Add alcohol and the remaining amount of the solution in step (1), and mix evenly to obtain a photosynthetic bacteria culture medium.

Embodiment 2

[0038] A simple and easy-to-make photosynthetic bacteria culture medium, each liter of culture medium includes the following components:

[0039]

[0040]

[0041] Make up to 1L with water.

[0042] The preparation method of photosynthetic bacteria culture medium:

[0043] (1) Take water to boil, cool to below 35°C for later use;

[0044] (2) Take nitrogen, phosphorus and potassium compound fertilizer, salt, monosodium glutamate, powdered brown sugar, and multivitamin B, add half the amount of water, and mix well;

[0045] (3) Add alcohol and the remaining amount of water, and mix evenly to obtain a photosynthetic bacteria culture medium.

Embodiment 3

[0047] A simple and easy-to-make photosynthetic bacteria culture medium, each liter of culture medium includes the following components:

[0048]

[0049] Make up to 1L with water.

[0050] The mango leaves, honeysuckle and purslane are all powdery medicinal materials passed through a sieve of 80-100 mesh.

[0051] The preparation method of photosynthetic bacteria culture medium comprises the following steps:

[0052] (1) Take water to boil, cool to below 35°C for later use;

[0053] (2) Take nitrogen, phosphorus and potassium compound fertilizer, salt, monosodium glutamate, brown sugar powder, vitamin B complex, mango leaves, honeysuckle and purslane, add half the amount of water, and mix well;

[0054] (3) Add alcohol and the remaining amount of water, and mix evenly to obtain a photosynthetic bacteria culture medium.

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Abstract

The invention discloses a simple and easy-to-prepare photosynthetic bacteria culture medium and a preparation method thereof. Each liter of the culture medium is prepared from the following componentsby weight: 1.0-2.0 g of nitrogen, phosphorus and potassium compound fertilizer, 4.0-8.0 g of table salt or crude salt, 0.3-0.5 g of monosodium glutamate, 0.1-0.5 g of brown sugar, 0.01-0.03 g of complex vitamins B, 75-100 ml of alcohol, and water added to be 1 L. The photosynthetic bacteria culture medium comprises suitable carbon sources, nitrogen sources, microelements and growth factors, has comprehensive nutrition, and can guarantee the normal growth demand of photosynthetic bacteria, and the components and the preparation method are simple, raw materials are easy to obtain and cheap, sources are stable, and the simple and easy-to-prepare photosynthetic bacteria culture medium is especially suitable for farmers to independently activate and expand cultivation of the photosynthetic bacteria.

Description

technical field [0001] The invention relates to a simple and easy-to-manufacture photosynthetic bacteria culture medium and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Photosynthetic bacteria is a general term for a class of prokaryotic microorganisms that use light as energy and can perform photosynthesis under anaerobic light or aerobic dark conditions. It plays an important role in water purification, water quality monitoring, feed additives, bacterial fertilizer, etc. Based on the application advantages of photosynthetic bacteria, it is often necessary to carry out large-scale cultivation of them. However, the medium used for photosynthetic bacteria generally has shortcomings such as long enrichment time, many types of medium components, and high cost, which are extremely limited. The application of photosynthetic bacteria in production. Contents of the invention [0003] In view of the deficiencies in the prio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/38C12R1/01
CPCC12N1/20C12N1/38
Inventor 张秀霞冼健安李军涛王冬梅郑佩华鲁耀鹏许嘉芮
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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