Single-channel fluorescence immunoassay micro-fluidic chip

Abstract

The invention discloses a single-channel fluorescence immunoassay micro-fluidic chip, and relates to the technical field of biological detection. The chip comprises a chip body which is provided witha closed micro-channel for a sample to flow. The micro-channel comprises a mixing bin, a reaction bin, a capture bin and a waste liquid bin which are sequentially communicated, and further comprises afirst vent hole communicated with the outside atmosphere and a sample adding port communicated with the mixing bin. A delay channel for communicating the reaction bin with the capture bin is arrangedbetween the reaction bin and the capture bin, and the delay channel is used for allowing the sample to flow in a zigzag manner. A solid reagent ball used for carrying out an immune reaction with thesample is arranged in the mixing bin, and the reagent ball contains an antibody / antigen coated with a fluorescent marker and a magnetic bead. A magnet used for adsorbing a conjugate generated by the immune reaction of the antibody and the antigen is arranged on the bottom wall of the capture bin. Thus, the immune reaction is fully carried out, the conjugate is captured and gathered in a flowing manner, and the correctness of the detection result is ensured. Moreover, the micro-channel is easy to produce, and the production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a single-channel fluorescent immunoassay microfluidic chip. Background technique [0002] Microfluidics is the integration of basic operating units such as sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes into a micron-scale chip to automatically complete the entire analysis process. Due to its great potential in the fields of biology, chemistry, and medicine, it has developed into a new research field interdisciplinary in biology, chemistry, medicine, fluid, electronics, materials, and machinery. At present, microfluidic chips have been applied in the field of immunoassay analysis. Immunofluorescence is a commonly used technique in the field of immunoassay analysis. [0003] Immunofluorescence technology refers to that based on the principle of antigen-antibody reaction, the known antigen or antibody i...

AI Technical Summary

Problems solved by technology

Although this technology enables the mixing, reaction, and separation of detection samples, fluorescent markers, and capture antibodies / antigens to be carried out in one device, in the prior art, the capture antibodies / antigens are coupled in the micro-channel while fluorescent The markers are solidified in the microfluidic channel and packaged and preserved in one piece, so that the fluorescent marker and the capture antibody / antigen will not undergo qualitative changes and can react with the sample when it flows through the channel, which increases the structural complexity of the microfluidic chip , Difficulty in production technology and difficulty in transportation and storage, increasing production costs; and because the immune reaction can only occur when the sample flows through the fluorescent site and the capture site, it is difficult to fully carry out the immune reaction. Fluorescently labeled antibodies / antigens and capture antibodies / antigens are fully reacted. Various complex structures are installed in the existing micro-channels to slow down the flow rate of the sample or increase the flow rate of the sample, or to react to the test after the reaction. Multi-filtering is performed on the samples to ensure the detection amount of the conjugates generated by the reaction, so as to avoid affecting the detection results, resulting in problems such as complex structure of the microfluidic chip, difficulty in production, low qualification rate of the produced microfluidic chip product, and high production cost.

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