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LF-RPA method for quickly identifying schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis and application thereof

A technology for oriental fluke and schistosomiasis, applied in the field of rapid identification of schistosomiasis japonicum, can solve problems such as large errors, and achieve the effect of simple operation, high sensitivity and rapid identification

Pending Publication Date: 2019-11-26
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI +1
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Problems solved by technology

For the feces incubation method commonly used in the detection of the existing schistosomiasis pathogen, the error of human subjective judgment is relatively large. If other more objective methods are used to identify miracidia, the diagnosis can be more accurate and symptomatic treatment can be given in time

Method used

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  • LF-RPA method for quickly identifying schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis and application thereof
  • LF-RPA method for quickly identifying schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis and application thereof
  • LF-RPA method for quickly identifying schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis and application thereof

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Embodiment Construction

[0029] Material

[0030] TwistAmp produced by British TwistDX company TM nfo Kits were purchased from TwistDX Company in the UK; lateral flow chromatography test strips (LF strip) produced by Milcnia Biotcc in Germany were purchased from Suzhou Damaidi Biomedical Technology Co., Ltd.; primers and probes were synthesized by Suzhou Jinweizhi Company and diluted to 10 μM for use ; All reagents used in the PCR amplification reaction system were purchased from Bioengineering (Dalian) Co., Ltd.; blood, cell, tissue genomic DNA extraction kits were purchased from Beijing Tiangen Biochemical Co., Ltd.

[0031] Primer design

[0032] Using the genome sequences of Schistosoma japonicum (GenBank: AF412221.1), Schistosoma mansoni (GenBank: M61098.1), and Orientobilax (GenBank: HQ283100.1) published by GenBank as templates, primers were designed and synthesized using Primer5 software.

[0033] Table 1, Primer Sequences

[0034]

[0035] Note: J: Schistosoma japonicum, M: Schistosoma ...

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Abstract

The invention discloses a LF-RPA method for quickly identifying schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis and application thereof and further discloses a quick detection kit and a detection method. A primer pair, probe and quick detection kit for detecting the schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis are established on the basis of molecular biology. The primer and probe can be used for specifically amplifying schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis, the kit for quickly detecting nucleicacids of schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis has the sensitivity higher than that of PCR, has strong specificity and high detection speed, and is extremely simple and convenient in result determination operation. The detection method and detection kit combined with an existing nucleic acid quick extraction method can achieve the aim of quickly identifyingand detecting schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis.

Description

technical field [0001] The invention relates to the technical field of parasite detection, in particular to an LF-RPA method for rapidly identifying Schistosoma japonicum, Schistosoma mansoni and Orientbilax and its application. Background technique [0002] Schistosomiasis is one of the oldest parasitic diseases of human beings. It has been recorded in China for more than 2,000 years. It is endemic in 74 countries and regions around the world. The population in endemic areas reaches 600 million, and 200 million people are infected. They die from this disease every year. There are as many as one million. In my country, the government attaches great importance to the prevention and control of schistosomiasis, and the national control plan has made great progress in controlling schistosomiasis. By the end of 2012, the estimated number of Schistosoma japonicum infections had decreased from 11.6 million in the 1950s to 240,000, and the number of endemic provinces had also decre...

Claims

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Application Information

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IPC IPC(8): C12Q1/6844C12Q1/6893C12N15/11
CPCC12Q1/6844C12Q1/6893C12Q2521/507C12Q2522/101C12Q2565/625
Inventor 朱传刚纪荣毅沈元曦周志平冒丽刘冀
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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