A combined method of using indigenous microorganisms and plants to remove lead pollution in phosphate rock wasteland

A technology of microorganisms and waste land, applied in the restoration of polluted soil and other directions, can solve the problems of heavy metal pollution, slow growth and difficult application in the waste land of phosphate rock that have not been found, and achieve good application prospects and promotion value, low cost, targeted at strong effect

Active Publication Date: 2022-01-11
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology can achieve the purpose of removing heavy metals from soil, but at the same time, microbial remediation technology also has some limitations, such as long remediation duration, slow effect, and the formation of heavy metal complexes cannot be completely removed from soil
Phytoremediation technology refers to the remediation technology that uses plants and their roots to remove heavy metal pollution in the soil through the absorption, volatilization, transformation, degradation and fixation of soil pollutants. This technology has the advantages of environmental friendliness and low cost. Some enrichment plants for remediation of heavy metal-contaminated soils usually grow slowly and have poor tolerance, which makes it difficult to apply this technology in practice.
[0005] At present, the research on the ecological restoration of phosphate rock wasteland is still in the exploratory stage at home and abroad. The main research work focuses on land reclamation and vegetation restoration. No indigenous microorganisms-plants related to heavy metal pollution in phosphate rock waste land, especially lead pollution have been found. Research Reports on Joint Removal

Method used

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  • A combined method of using indigenous microorganisms and plants to remove lead pollution in phosphate rock wasteland
  • A combined method of using indigenous microorganisms and plants to remove lead pollution in phosphate rock wasteland
  • A combined method of using indigenous microorganisms and plants to remove lead pollution in phosphate rock wasteland

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] After sterilizing 500g of uncontaminated common soil, mix it with 20-50g of low-grade phosphate rock powder evenly, then spray lead nitrate solution with a concentration of 80-120mg / L, and stabilize it for 2-4 weeks to obtain a simulated soil sample. The microbial inoculum selected for inoculation is the indigenous high-efficiency lead-dissolving phosphorus-resistant microbial bacterial solution obtained by screening in step (b), namely the Pseudomonas bacterial solution. The bacterial solution was cultured to logarithmic phase before use.

[0029] Control group: 500g of sterilized simulated soil samples were put into plastic flowerpots with a diameter of about 11cm, and then ryegrass seedlings with a height of 5-6cm and chicory seedlings with a height of 1-2cm were transplanted to the same flower In pots, each pot is guaranteed to have 3-4 plants. Considering that the suitable cultivation temperature of the two plants is 20-35°C, cultivate at this temperature and wate...

Embodiment 2

[0038] Considering that most of the lead in the soil is in the form of insoluble PbCO 3 exists, by adding a certain amount of PbCO to ordinary soil 3 way to simulate lead-contaminated soil. Mix 500g of uncontaminated common soil (sterilized in advance), 20-50g of low-grade phosphate rock powder and 1-3g of PbCO 3 Mix evenly to obtain a simulated soil sample.

[0039] Control group: 500g of sterilized simulated soil samples were put into a plastic flowerpot with a diameter of about 11cm, and then ryegrass seedlings with a height of 5-6cm and chicory seedlings with a height of 1-2cm were transplanted into the same flowerpot medium, 3-4 plants are guaranteed in each pot. The cultivation temperature is controlled at 20-35°C, the water content in the soil is kept at 60%-80% of the maximum water holding capacity in the field, and the bacterial liquid is not inoculated.

[0040] Experimental group: 500g of sterilized simulated soil samples were put into a plastic flowerpot with a...

Embodiment 3

[0048] Using the screened indigenous high-efficiency lead-tolerant phosphorus-dissolving microorganisms Pseudomonas and indigenous lead-enriching plants ryegrass and chicory as materials, and taking lead-contaminated phosphate rock waste soil samples (real soil samples) as the removal objects, the research was carried out. corresponding test.

[0049] Control group: 500g of sterilized real soil samples were put into plastic flowerpots with a diameter of about 11cm, and then ryegrass seedlings with a height of 5-6cm and chicory seedlings with a height of 1-2cm were transplanted to the same flower In pots, each pot is guaranteed to have 3-4 plants. Considering that the suitable cultivation temperature for both plants is 20-35°C, cultivate at this temperature and water regularly, keep soil water content at 60%-80% of the maximum water holding capacity in the field, and do not inoculate bacterial liquid.

[0050] Experimental group: put 500g of sterilized real soil samples into p...

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Abstract

The present invention relates to a method for removing lead pollution in phosphate rock waste land by utilizing indigenous microorganism-plants. First, the indigenous high-efficiency lead-soluble phosphorus-tolerant microorganisms and indigenous lead-enriched plants were screened from the lead-contaminated phosphate rock wasteland, and then the indigenous lead-enriched plants were transplanted into the lead-contaminated phosphate rock wasteland soil, and then the indigenous highly efficient The lead-soluble phosphorus-tolerant microorganisms are inoculated into the rhizosphere soil of indigenous lead-enriched plants, and they can be co-cultivated for a period of time. The indigenous high-efficiency lead-resistant phosphorus-dissolving microorganisms and indigenous lead-enriching plants used in the present invention both come from the phosphate rock waste land, can well adapt to the ecological environment of the phosphate rock waste land, and can effectively remove the phosphate rock waste land through the combined action of the two. Lead contamination and excess phosphorus. The invention has the advantages of simple process, low production cost, no secondary pollution, over 80% restoration efficiency of lead pollution in phosphate rock abandoned land, and very good application prospect and popularization value.

Description

technical field [0001] The invention relates to the technical field of ecological restoration and heavy metal pollution control in phosphate rock waste land, in particular to a method for removing lead pollution in phosphate rock waste land by utilizing indigenous microorganisms and plants jointly. Background technique [0002] my country is a country rich in phosphate rock resources. The reserves of phosphorus resources are second only to Morocco in the world. However, my country's phosphate rock resources show that rich ores are easy to beneficated, and poor ores are difficult to beneficated. The rich and the poor have formed a large number of waste phosphate mines. The soil of phosphate rock wasteland contains a certain amount of heavy metal elements, such as lead, cadmium, chromium, mercury, arsenic and so on. These heavy metal elements will migrate in the long-term rainwater leaching process, causing serious pollution of the atmosphere, water and soil, further causing l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10
CPCB09C1/10B09C1/105
Inventor 肖春桥王琪张华香池汝安
Owner WUHAN INSTITUTE OF TECHNOLOGY
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