Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for testing and regulating the function of cardiomyocytes in vitro by means of force stimulation

A cardiomyocyte and functional testing technology, applied in the fields of biomechanics and mechanics, biology, medicine and engineering, can solve the problems of sample clamp separation, uneven application of mechanical excitation, and difficulty in realizing slow variable radius twisting loading, etc. Low, easy to control and analyze effects

Active Publication Date: 2020-12-29
THE FIRST PEOPLES HOSPITAL OF CHANGZHOU +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] When studying the physiological response of cardiomyocytes in a force-electric coupling environment, specific excitation and cell function testing devices are usually required, but most of the current devices are of a single type, and there are mechanical excitations for cell culture in gels. Problems such as inhomogeneity, separation of sample holder and force loading device, and difficulty in realizing slow variable radius torsional shear loading and ultra-small torsional shear strain

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for testing and regulating the function of cardiomyocytes in vitro by means of force stimulation
  • A method for testing and regulating the function of cardiomyocytes in vitro by means of force stimulation
  • A method for testing and regulating the function of cardiomyocytes in vitro by means of force stimulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Such as figure 1 As shown, this embodiment 1 provides a method for testing and regulating the function of cardiomyocytes in vitro by means of force stimulation, including: step S1, applying force stimulation to the overall gel that wraps cardiomyocytes to collect the stretching and Migration data; step S2, according to the data collected in step S1, selectively take a part of the whole gel, and apply force-electrical coupling stimulation to the local gel, so as to collect data on the contraction force and contraction frequency of cardiomyocytes; and step S3 , to degrade the gel to obtain the cell suspension, so as to collect the parameter data of the cell suspension.

[0035] Further, in step S1, the method of applying force stimulation to the whole gel wrapped with cardiomyocytes to collect the stretching and migration data of cardiomyocytes includes: using a force stimulation loading device to apply preset strain loading to the whole gel, including tensile stress , e...

Embodiment approach

[0050] Such as Figure 4 As shown, the container includes: an upper cover 1 and a lower cover 3, and one side of the upper cover 1 is connected to the stretching mechanism through a connecting card cover, and one side of the lower cover 3 is connected through another connecting card. The cover is connected with the stretching mechanism; the upper cover plate 1 and the lower cover plate 3 are made of elastic rubber; the inner surface of the upper cover plate 1 is provided with several first protrusions 11 at intervals; and the lower cover plate 3 Several second protrusions 31 are arranged at intervals on the inner surface of the inner surface.

[0051] Further, the stretching mechanism includes: screw mechanisms respectively arranged symmetrically on opposite sides of the containing body; the screw mechanisms include: screw motors (40; 50), transmission shafts (41; 51), wire A rod (42; 52) and a nut; wherein the screw rod (42; 52) passes through the nut, and one end is connect...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of medicine and engineering crossover, in particular to a method for carrying out myocardial cell in-vitro function testing, regulation and control by a force stimulation way. The method comprises the following steps of: S1: applying force stimulation to an integral gel which covers a myocardial cell to collect the extension and migration data of the myocardial cell; S2: taking the local part of the integral gel, applying force-electrical coupling type stimulation to the local gel to collect the contractility and the contraction frequency data of the myocardial cell; and S3: degrading the gel to obtain a cell suspension, and collecting the parameter data of the cell suspension. By use of the method, through a force stimulation loading device, a strain orstress is applied to the integral gel, a tensile stress, an expression stress and a twisting and cutting stress are simultaneously applied to the local gel through selection after the growth of the cell is observed, the myocardial cell can feel static and dynamic mechanical stimulation in a cell micro-environment through a force sensitive ion channel on a cell membrane, and electrophysiology on the cell membrane and an intracellular biological chemistry response are activated so as to perform a very important function on regulating and controlling the structure and the function of the myocardial cell.

Description

technical field [0001] The invention belongs to the interdisciplinary field of medicine and engineering, in particular to the fields of biomechanics and mechanobiology, and specifically relates to a method for in vitro function testing and regulation of cardiomyocytes by means of force stimulation. Background technique [0002] Cardiovascular disease is currently the leading cause of human death worldwide, and the development of myocardial tissue engineering provides the most potential solution for the treatment of cardiovascular disease. During the occurrence and development of cardiovascular diseases, it is closely related to the changes of cell force-electrical microenvironment. In the past ten years, with the development of advanced biomaterials and micro-nano biomanufacturing technologies, more and more studies have shown that the regulation of cell force-electrical microenvironment plays an important role in the maturation and functionalization of engineered myocardial...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q3/00C12M1/00C12M1/42
CPCC12M35/02C12M35/04C12Q3/00
Inventor 施媛萍朱晓璐王郑尹曦印强
Owner THE FIRST PEOPLES HOSPITAL OF CHANGZHOU