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A method for analyzing isomaltooligosaccharides and its isomers in yogurt

A technique for isomalto-oligosaccharides and isomers, applied in the field of chemical analysis, can solve the problems of high detection limit, inability to effectively distinguish panose and isomalt, and achieve high sensitivity, high efficiency and strong selectivity. Effect

Active Publication Date: 2022-06-28
广东省科学院生物与医学工程研究所
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Problems solved by technology

The current standard method for detecting SN / T3637-2013 uses the HPLC-RID method, but there are defects such as the inability to effectively distinguish panose and isomaltotriose, and the high detection limit. How to conduct qualitative and quantitative analysis for more effective separation of isomaltooligosaccharides has been a challenge for food analysts
At present, there is no research on the detection of isomaltooligosaccharides in food by ultra-high performance liquid chromatography-ion mobility-quadrupole tandem time-of-flight mass spectrometry

Method used

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  • A method for analyzing isomaltooligosaccharides and its isomers in yogurt
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  • A method for analyzing isomaltooligosaccharides and its isomers in yogurt

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[0039] specific implementation

[0040] In order to facilitate the understanding of those skilled in the art, the present invention will be further described below with reference to the embodiments, and the contents of the embodiments are not intended to limit the present invention.

[0041] A method for analyzing isomaltose and its isomers in yogurt, comprising the following steps:

[0042] S1. Configuration of standard solution: Accurately weigh 10.00 mg of standard products of maltose, maltotriose, isomaltose, panose and isomaltose, dissolve them in 10 mL volumetric flasks with ultrapure water and dilute to 10 mL, and prepare 1000mg / L of 5 standard single-label stock solutions; then take 0.1mL of each 1000mg / L standard single-label stock solution, dilute to 10mL with ultrapure water in a 10mL volumetric flask, and obtain 10mg / L of 5 kinds of oligomers The standard mixed standard stock solution of sugar was stored in a refrigerator at 4°C; the above stock solution was dilut...

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Abstract

The invention relates to an analysis method of isomaltooligosaccharide and its isomers in yoghurt. By applying ultra-high performance liquid chromatography-ion mobility-triple quadrupole mass spectrometry tandem time-of-flight mass spectrometry technology, HMS is used E mode to collect samples, use primary and secondary ion fragments for quantification, use retention time and ion travel time for qualitative analysis, and accurately analyze maltose, maltotriose and isomaltose, panose and isomaltose in yogurt in multiple dimensions Maltotriose, established the UPLC-IMS-QTof method for oligomeric isomalt in yogurt, filling the gap in this field, providing more efficient and accurate analysis methods for workers in this industry, and providing technology for the detection of oligosaccharide content in yogurt on the market support.

Description

technical field [0001] The invention relates to the field of chemical analysis, in particular to a method for determining the content of isomaltose oligosaccharides and isomers thereof. Background technique [0002] Isomaltose (IMO) refers to a class of oligosaccharides formed by the combination of two or more glucoses with α-1,6-glycosidic bonds. The most common foods in food are: Isomaltose (IG2), panose (Panose, P), Isomaltotriose (Isomaltotriose, IG3), etc., due to different molecular conformations, in order to distinguish it from maltose, it is called isomaltose oligosaccharide. Isomalt oligosaccharide has excellent heat resistance and acid resistance. The syrup with a concentration of 50% will not be differentiated when heated for a long time at PH3 and 120 °C. The water activity of isomalt oligosaccharide at 75% and 25 °C is 0.75, which is close to Because of sucrose, general bacteria, yeasts and molds cannot grow in the environment with AW≤0.8, so it has excellent a...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/36G01N30/86
CPCG01N30/02G01N30/36G01N30/8675
Inventor 李硕聪高裕锋甄振鹏黄敏兴庞然海王小鹏叶锦梅余构彬王桂华郭剑雄
Owner 广东省科学院生物与医学工程研究所
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