Method for analyzing isomalto-oligosaccharide and isomeride thereof in yoghourt

A technology of isomaltooligosaccharides and isomers, which is applied in the field of chemical analysis, can solve the problems of high detection limit and inability to effectively distinguish panose and isomaltotriose, and achieve high sensitivity, high efficiency and strong selectivity Effect

Active Publication Date: 2019-12-10
广东省科学院生物与医学工程研究所
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Problems solved by technology

The current standard method for detecting SN / T3637-2013 uses the HPLC-RID method, but there are defects such as the inability to effectively distinguish panose and isomaltotriose, and the high detection limit. How to conduct qualitative and quantitative analysis for more effective separation of isomaltooligosaccharides has been a challenge for food analysts
At present, there is no research on the detection of isomaltooligosaccharides in food by ultra-high performance liquid chromatography-ion mobility-quadrupole tandem time-of-flight mass spectrometry

Method used

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  • Method for analyzing isomalto-oligosaccharide and isomeride thereof in yoghourt
  • Method for analyzing isomalto-oligosaccharide and isomeride thereof in yoghourt
  • Method for analyzing isomalto-oligosaccharide and isomeride thereof in yoghourt

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[0039] specific implementation plan

[0040] In order to facilitate the understanding of those skilled in the art, the present invention will be further described below in conjunction with the examples, which are not intended to limit the present invention.

[0041] A method for analyzing isomaltooligosaccharides and isomers thereof in yogurt, comprising the following steps:

[0042] S1. Configuration of standard solution: Accurately weigh 10.00 mg each of maltose, maltotriose, isomaltose, panose, and isomaltotriose standard, dissolve them in 10mL volumetric flasks with ultrapure water and dilute to 10mL, and prepare 5 kinds of standard single standard stock solution of 1000mg / L; then take 0.1mL of each standard single standard stock solution of 1000mg / L respectively, and dilute to 10mL with ultrapure water in a 10mL volumetric flask to obtain 10mg / L of five kinds of oligomeric The standard mixed standard stock solution of sugar was stored in a refrigerator at 4°C; the above ...

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Abstract

The invention relates to a method for analyzing isomalto-oligosaccharide and isomeride thereof in yoghourt. The method comprises the following steps: applying an ultra-high performance liquid chromatography-ion mobility-triple quadrupole mass spectrometry tandem time-of-flight mass spectrometry technology; using an HMSE mode for collecting a sample; using primary and secondary ion fragments for quantification; determining the nature by using the retention time and the ion drift time; accurately analyzing maltose, maltotriose, isomaltose, panose and isomaltotriose in the yoghourt from multipledimensions; establishing the UPLC-IMS-QTof method for isomalto-oligosaccharide in yoghourt, the blank in the field is filled, a more efficient and accurate analysis method is provided for workers in the industry, and technical support is provided for detecting the oligosaccharide content of yoghourt on the market.

Description

technical field [0001] The invention relates to the field of chemical analysis, in particular to a method for determining the content of isomaltooligosaccharide and its isomers. Background technique [0002] Isomaltose (IMO) refers to a type of oligosaccharides formed by combining two or more glucose with α-1,6-glucosidic bonds. The common ones in food mainly include: Isomaltose (IG2), panose (Panose, P), isomaltotriose (Isomaltotriose, IG3), etc., are called isomaltooligosaccharides in order to distinguish them from maltose due to their different molecular conformations. Isomaltooligosaccharide has excellent heat resistance and acid resistance. The syrup with a concentration of 50% will not decompose even if it is heated for a long time at PH3 and 120°C. In addition to sucrose, general bacteria, yeast, and mold cannot grow in the environment of AW≤0.8, so it has excellent antiseptic effect; in addition, isomaltooligosaccharides added to bread and fermented milk (yogurt) wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/36G01N30/86
CPCG01N30/02G01N30/36G01N30/8675
Inventor 李硕聪高裕锋甄振鹏黄敏兴庞然海王小鹏叶锦梅余构彬王桂华郭剑雄
Owner 广东省科学院生物与医学工程研究所
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