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Application of Citrobacter sp. in removal of dye wastewater

A technology for Citrobacter bacillus and dye wastewater, applied in the field of microorganisms, can solve the problems of difficult treatment, poor biochemical properties, decolorization of acidic golden yellow G and bromocresol violet, etc., and achieves the effects of efficient removal and low economic cost.

Inactive Publication Date: 2019-12-13
NORTHEAST NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difficulty in treating acidic golden G wastewater is high chemical oxygen demand, high chroma, and poor biochemical properties
Its chemical properties are relatively stable, and the composition of the wastewater is complex, so the wastewater is recognized as difficult-to-treat organic wastewater
At present, there is no patent report on the decolorization of acidic golden yellow G and bromocresol purple in China
And also do not see the patent report that Citrobacter is used for dye decolorization at present

Method used

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  • Application of Citrobacter sp. in removal of dye wastewater
  • Application of Citrobacter sp. in removal of dye wastewater
  • Application of Citrobacter sp. in removal of dye wastewater

Examples

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Effect test

Embodiment 1

[0034] Embodiment 1: Research on the removal of acid dye bromocresol purple wastewater by facultative anaerobic bacteria Citrobacter sp.JPG1 under aerobic conditions

[0035]Inoculate the facultative anaerobic bacterium Citrobacter sp.JPG1 into 100ml of sterilized LB medium, culture it in an incubator at 30°C and 120rpm for 12h, and transfer it when the OD600 is 1. 10% of the ratio was inserted into 200ml of simulated sterilized LB medium containing 200mg / L bromocresol purple dye wastewater, and the removal experiment was carried out on a shaker at 120rpm and 30°C under aerobic conditions. After cultivating for a period of time, , aspirate 5ml of culture solution, centrifuge at 10000rpm for 4min, and take the supernatant to measure the absorbance value. The results found that when the bromocresol purple dye wastewater concentration was 200mg / L, the initial inoculum size of the bacterial solution was 10%, and the p-bromocresol purple dye wastewater reached a removal rate of 73....

Embodiment 2

[0036] Embodiment 2: Research on the removal of azo dye acid golden yellow G wastewater under anaerobic conditions by facultative anaerobic bacteria Citrobacter sp.JPG1

[0037] The preserved facultative anaerobic bacterium Citrobacter sp.JPG1 was inoculated into 100ml of sterilized LB medium, cultured in an incubator at 30°C and 120rpm for 12h, and transferred when the OD600 was 1. When transferring, insert 200ml of simulated sterilized LB medium containing 200mg / L acidic golden yellow G wastewater at a ratio of 10%. After culturing for a period of time, absorb 5ml of the culture solution, centrifuge at 10000rpm for 4min, and take the supernatant to measure the absorbance. like Figure 4 Shown, the result finds that when the acid golden yellow G wastewater concentration is 200mg / L, the initial inoculation amount of bacterial solution is 10%, reaches 87.51% removal rate to acid golden yellow G wastewater in the time of 36h. After 60 hours, Citrobacter sp.JPG1 had a good rem...

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Abstract

The invention discloses an application of Citrobacter sp. JPG1 in removal of bromcresol purple and acid golden yellow G, and belongs to the technical field of microorganisms. The Citrobacter JPG1 is preserved in China General Microbiological Culture Collection Center on December 22, 2016 with the preservation number of CGMCC NO.13480, and the strain has good ability to remove the bromcresol purpleunder an aerobic condition and the acid golden yellow G under an anaerobic condition. When the respectively concentration of the bromcresol purple and the acid golden yellow G is 200 mg / L and the initial inoculum size of the strain is 10%, the removal rate of the p-bromocresol purple at 36 h reaches 73.64%, and the removal rate of the acid golden yellow G at 36 h reaches 87.51%. The Citrobactersp. JPG1 is an efficient dye removal bacterium, can be directly applied to biological treatment of dye wastewater containing bromcresol purple and acid golden yellow G, and has good application prospects in anaerobic and aerobic treatments.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an application of Citrobacter in removing dye wastewater. Background technique [0002] Dyes refer to a class of organic compounds that can make other substances obtain bright and firm colors. The waste water generated in the textile printing and dyeing process causes water environment pollution because half of the dyes are not used. Discharge of untreated dye wastewater into water can lead to eutrophication and interference with light absorption and reflection in the aquatic environment, as well as teratogenic, carcinogenic and mutagenic effects on aquatic organisms and humans. [0003] At present, there are chemical, physical and biological methods for treating dye wastewater at home and abroad. Chemical methods are generally divided into oxidation method and electrolysis method, and the sludge produced is difficult to handle and easy to cause secondary pollution; physi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/30C12N1/20C12R1/01C02F101/30C02F101/34C02F101/36C02F101/38
CPCC02F3/30C02F3/34C02F2101/308C02F2101/345C02F2101/36C02F2101/38C12N1/205C12R2001/01
Inventor 王小雨安双双
Owner NORTHEAST NORMAL UNIVERSITY
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