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Euphausia superba oxidation resisting oligopeptide and preparation method thereof

A technology of Antarctic krill and Antarctic krill powder, which is applied in the biological field, can solve problems such as liver damage, and achieve the effects of improving vitality, reducing content, and good scavenging effect

Active Publication Date: 2019-12-13
德州蓝力生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemically synthesized antioxidants damage the liver, kidneys and other organs of the human body to varying degrees, and some countries and regions have clearly limited or banned their use
[0003] The present invention uses Antarctic krill as a raw material, and the technology research of preparing Antarctic krill antioxidant oligopeptides by using enzymatic hydrolysis technology is in a blank stage, and the preparation of highly active antioxidant peptides and their applications using Antarctic krill enzymatic hydrolysis products as materials is even more unexplored. see the report

Method used

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  • Euphausia superba oxidation resisting oligopeptide and preparation method thereof
  • Euphausia superba oxidation resisting oligopeptide and preparation method thereof
  • Euphausia superba oxidation resisting oligopeptide and preparation method thereof

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Effect test

Embodiment 1

[0024] A preparation method of Antarctic krill antioxidant oligopeptide:

[0025] S1. Degreasing: using Antarctic krill as raw material, through degreasing, the method of degreasing is to wash the shells of Antarctic krill, crush them, grind them finely, and use low-temperature continuous phase change technology to extract shrimp oil. The obtained extraction residue is dried and sieved. It is defatted Antarctic krill meal;

[0026] The specific method of the low-temperature continuous phase change technology is: put Antarctic krill shell powder into a low-temperature continuous phase-change extraction kettle and seal it, set relevant extraction parameters for low-temperature continuous phase-change extraction operation, the extraction temperature is 37°C, and the extraction pressure is 1.2MPa, the extraction time is 50min, and the analysis temperature is 60°C;

[0027] S2. Enzymolysis: enzymolysis is multi-stage enzymolysis, the method is that 10g of defatted Antarctic krill ...

Embodiment 2

[0031] A preparation method of Antarctic krill antioxidant oligopeptide:

[0032] S1. Degreasing: using Antarctic krill as raw material, through degreasing, the method of degreasing is to wash the shells of Antarctic krill, crush them, grind them finely, and use low-temperature continuous phase change technology to extract shrimp oil. The obtained extraction residue is dried and sieved. It is defatted Antarctic krill meal;

[0033] The specific method of the low-temperature continuous phase change technology is: put Antarctic krill shell powder into a low-temperature continuous phase-change extraction kettle and seal it, set relevant extraction parameters for low-temperature continuous phase-change extraction operation, the extraction temperature is 37°C, and the extraction pressure is 1.2MPa, the extraction time is 50min, and the analysis temperature is 60°C;

[0034] S2. Enzymatic hydrolysis: enzymatic hydrolysis is multi-stage enzymatic hydrolysis. The method is to redisso...

Embodiment 3

[0038]A preparation method of Antarctic krill antioxidant oligopeptide:

[0039] S1. Degreasing: using Antarctic krill as raw material, through degreasing, the method of degreasing is to wash the shells of Antarctic krill, crush them, grind them finely, and use low-temperature continuous phase change technology to extract shrimp oil. The obtained extraction residue is dried and sieved. It is defatted Antarctic krill meal;

[0040] The specific method of the low-temperature continuous phase change technology is: put Antarctic krill shell powder into a low-temperature continuous phase-change extraction kettle and seal it, set relevant extraction parameters for low-temperature continuous phase-change extraction operation, the extraction temperature is 37°C, and the extraction pressure is 1.2MPa, the extraction time is 50min, and the analysis temperature is 60°C;

[0041] S2. Enzymatic hydrolysis: enzymatic hydrolysis is multi-stage enzymatic hydrolysis. The method is to redissol...

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Abstract

The invention provides a preparation method of euphausia superba oxidation resisting oligopeptide. Euphausia superba is used as a raw material, through defatting, enzymolysis, ultrafiltration, cationexchange resin chromatography, gel column chromatography and antiphase high performance liquid chromatogram separation and purification, the oxidation resisting oligopeptide is obtained. The preparedhigh activity oxidation resisting peptide disclosed by the invention can have favorable elimination effects on DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals, can significantly increase the vitality of SOD and GSH-Px in HUVEC cells, and can reduce the contents of intracellular NO and MDA.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an Antarctic krill antioxidant oligopeptide and a preparation method thereof. Background technique [0002] Antioxidants are a class of substances that can help capture and neutralize free radicals to eliminate the damage caused by free radicals to the human body. They are food additives that can prevent or delay food oxidation, improve food stability and extend storage life. At present, chemically synthesized antioxidants, such as butyl hydroxyanisole, dibutyl hydroxytoluene, propyl gallate, tert-butyl hydroquinone, etc., are widely used in the food industry. However, chemically synthesized antioxidants damage the liver, kidneys and other organs of the human body to varying degrees, and some countries and regions have clearly limited or banned their use. [0003] The present invention uses Antarctic krill as a raw material, and the technology research of preparing Antarctic krill a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16A61K38/08A61P39/06A23L33/18
CPCA23V2002/00A61K38/00A23L33/18A61P39/06C07K7/06C12P21/06A23V2200/30A23V2250/55
Inventor 王斌赵文浩赵玉勤迟长凤
Owner 德州蓝力生物技术有限公司
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