Efficient chemical biological preparation for removing pseudomonas aeruginosa biofilm and application thereof

A technology for chemical biological preparations and microbial preparations, which is applied in the field of high-efficiency chemical biological preparations, can solve the problems of cumbersome preparation process of gallium-containing compositions and is not suitable for commercial production, and achieves the effects of low price, low cost and good effect.

Active Publication Date: 2019-12-20
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing methods for removing and inhibiting biofilm formation: the Chinese invention patent with the notification number CN105613584B discloses a method for improving the removal and sterilization efficiency of silver ions on biofilm, through a small molecular substance that has a dissociation effect on biofilm Bis(3-aminopropyl)amine is coupled with silver ions, which improves the efficiency of inorganic silver ions on biofilm removal and sterilization, but the raw materials of this invention contain high-cost materials such as inorganic silver ions and bis(3-aminopropyl)amine , not suitable for commercial production; the Chinese invention patent with the notification number CN1901801B discloses that gallium inhibits the formation of biofilms, and coating the equipment or equipment surface with a gallium-containing composition at a concentration sufficient to inhibit the formation of biofilms has a significant effect, but The preparation process of the gallium-containing composition is cumbersome

Method used

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  • Efficient chemical biological preparation for removing pseudomonas aeruginosa biofilm and application thereof
  • Efficient chemical biological preparation for removing pseudomonas aeruginosa biofilm and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A high-efficiency chemical biological agent for removing Pseudomonas aeruginosa biofilm, based on the total mass of 100%, includes the following components:

[0048] ΔLasR strain + ΔLasI strain 70%,

[0049] N-Acetyl-L-cysteine ​​24%,

[0050] Catalase 6%.

[0051] Wherein, the mass ratio of the ΔLasR strain to the ΔLasI strain is 90:10.

Embodiment 2

[0053] A high-efficiency chemical biological agent for removing Pseudomonas aeruginosa biofilm, based on the total mass of 100%, includes the following components:

[0054] ΔLasR strain + ΔLasI strain 65%,

[0055] N-Acetyl-L-cysteine ​​26%,

[0056] Catalase 9%.

[0057] Wherein, the mass ratio of the ΔLasR strain and the ΔLasI strain is 85:15.

Embodiment 3

[0059] A high-efficiency chemical biological agent for removing Pseudomonas aeruginosa biofilm, based on the total mass of 100%, includes the following components:

[0060] ΔLasR strain 70%,

[0061] N-Acetyl-L-cysteine ​​24%,

[0062] Catalase 6%.

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Abstract

The invention discloses an efficient chemical biological preparation for removing a pseudomonas aeruginosa biofilm. The efficient chemical biological preparation comprises the following components based on 100% of total mass percentage of 60-75% of a microbial preparation and 25-40% of an active oxygen free radical scavenger, wherein the microbial preparation is a delta LasR strain and / or a deltaLasI strain. The efficient chemical biological preparation for removing the pseudomonas aeruginosa biofilm has the advantages of being green, economical, pervasive, long-lasting and the like, the formula is simple, the used ingredients are low in price and wide in source, and the efficient chemical biological preparation is low in cost and good in effect, and capable of suppressing growth of pseudomonas aeruginosa and film formation.

Description

technical field [0001] The invention relates to the field of membrane pollution removal, in particular to a high-efficiency chemical biological agent for removing Pseudomonas aeruginosa biofilm and application thereof. Background technique [0002] Biofilms, also known as biofilms, are aggregates of organized growth of microorganisms. Composed of lipids, proteins, and sugars, bacterial biofilms confer strong physical resistance to antibiotics and facilitate the disruption of host immune defense mechanisms. When biofilm forms on domestic pipelines, industrial production, and medical equipment, it will cause erosion and blockage of pipelines, pollute products, reduce heat conduction, and cause bacterial infections, etc., which will bring many problems to production and life. [0003] Pseudomonas aeruginosa (P.a) is widely distributed in nature and exists in various moist environments, such as water, air, human skin, respiratory tract and intestinal tract. In many actual prod...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N37/46A01P1/00
CPCA01N37/46A01N63/00
Inventor 汪美贞肖俊炜孙峰李张强单玉菊冯华军沈东升
Owner ZHEJIANG GONGSHANG UNIVERSITY
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