Fully human monoclonal antibody for neutralizing enterovirus 71 (EV71) and application of fully human monoclonal antibody

Abstract

The invention belongs to the field of biological immunology, and more particularly relates to a fully human monoclonal antibody for neutralizing enterovirus 71 (EV71) and application of the fully human monoclonal antibody. An amino acid sequence of a heavy chain variable region is shown as any one of SEQ ID NO:14-20, and an amino acid sequence of a light chain variable region is shown as any one of SEQ ID NO:21-26. The invention further provides a nucleic acid molecule encoding the antibody, an expression vector, application and a composition. Binding molecules can prevent the EV71 from infecting susceptible cells and are fully human derived, compared with other animal derived (such as rat-derived) anti-EV71 molecules, immunogenicity is low, affinity is good, the treating effect is good, and side effects are low.

Description

technical field [0001] The invention relates to the field of biological immunology, in particular to a fully human monoclonal antibody neutralizing enterovirus 71 and its use. Background technique [0002] Hand foot mouth disease (HFMD) is a common acute infectious disease of infants and young children caused by enterovirus. Small herpes or small ulcers appear in other parts, most of the children will heal by themselves in about 1 week, and a few children may cause complications such as myocarditis, pulmonary edema, aseptic meningitis, etc., and the rapid development of severe children may lead to death. Currently, there is no effective treatment. medicine. In recent decades, HFMD has broken out and become popular in many parts of the world. In 2008, my country included hand, foot and mouth disease as a category C infectious disease under statutory report management, requiring it to be reported to the Ministry of Health within 24 hours. From 2009 to 2015, my country's heal...

AI Technical Summary

Problems solved by technology

[0005] In the first half of 2016, the vaccine against EV71 virus has been approved for marketing. This is currently the only vaccine that can be used to prevent hand, foot and mouth disease. It can curb the incidence of hand, foot and mouth disease caused by EV71 virus to a certain extent, but there are some limitations : People over the age of 5, patients with fever, patients with acute and chronic disease attacks, and those with allergies should not receive the vaccine; those who are allergic to the active substance of the vaccine and kanamycin sulfate should not be vaccinated; the vaccination has individual differences and can Some adverse effects; does not protect against all genotypes of EV71 virus, not effective for patients already infected with EV71 virus

However, ribavirin and other nucleoside analogues can easily induce adverse reactions such as hemolytic anemia, and have a certain potential inhibitory effect on the growth and development of infants, so the benefits and risks should be carefully weighed when used for treatment

Although the traditional Chinese medicines used have the effect of clearing heat and detoxifying, they are all broad-spectrum heat-clearing and detoxifying drugs, which are not very targeted, and their direct action mechanism is not yet clear

In summary, there is currently a lack of specific, specific, and mechanism-specific anti-EV71 drugs, so it is of great significance to develop fully human monoclonal antibodies with high specificity against EV71 virus

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