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A new method and application of producing d-lactic acid by fermenting straw with Pyrocystis sp.

A technology of Pyroflagellate and straw, which is applied in the field of biotechnology and renewable energy, can solve the problems of high pollution, low output, high consumption, etc., and achieve the effects of simple fermentation process, improved economic benefits, and increased utilization rate

Active Publication Date: 2020-07-14
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, a large number of corn stalk resources in rural areas of our country are completely in a state of high consumption, high pollution, low utilization rate, and low output. Corn stalks have not been rationally developed and utilized as an energy source.
Corn stalks can be regenerated as resources through anaerobic digestion treatment, but the existing anaerobic digestion technology has problems of low efficiency and difficulty in popularization

Method used

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  • A new method and application of producing d-lactic acid by fermenting straw with Pyrocystis sp.

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Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1, the preparation of pirocystis spirochete Piromyces CY1 inoculum

[0035]Draw 1mL of Piromyces CY1 culture and inoculate it into a 20mL Heinz anaerobic tube with air-dried and pulverized wheat straw as a substrate in 9mL of anaerobic medium, and at the same time add compound antibiotics to make penicillin and streptomycin sulfate The final concentrations of chloramphenicol were 1600IU / mL and 2000IU / mL respectively, and the final concentration of chloramphenicol in the culture medium was 50μg / mL. After anaerobic culture at 39°C for 72 hours, the growth peak is reached, and the fermentation broth is a high-activity bacterial agent at this time.

Embodiment 2

[0036] Example 2, the method for producing D-lactic acid by fermenting straw with Piromyces CY1

[0037] 1. The method of anaerobic fermentation straw production D-lactic acid

[0038] 90 mL of liquid basic medium was placed in a 100 mL volume anaerobic fermentation bottle, and 1.0 g of crushed and air-dried wheat straw, corn straw and rice straw were respectively used as substrates. Deoxygenation. Sterilize. Inoculate 10 mL of Piromyces CY1 subcultured for 72 hours into the above-mentioned anaerobic medium with wheat straw, corn straw and rice straw as substrates, and compound antibiotics were added to make it grow in anaerobic environment. The final concentration of the medium solution was 1600 IU / mL of penicillin and 2000 IU / mL of streptomycin sulfate, the final concentration of chloramphenicol in the medium was 50 μg / mL, and cultured anaerobically at 39° C. for 7 days. A total of 3 parallel experiments were set up, and the production of D-lactic acid in the fermentation...

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Abstract

The invention relates to the field of biotechnology renewable energy, in particular to a method and application for producing D-lactic acid by fermenting straws by Pyrocystis sp. The invention discloses a method for producing D-lactic acid by anaerobically fermenting straw of Piromyces CY1 and its application in preparing D-lactic acid. The Piromyces CY1 is preserved in the China General Microorganism Culture Collection and Management Center, and the preservation number is: CGMCC NO.18141. It is also disclosed that the Piromyces disclosed by the present invention can survive in vitro through preservation. Fermenting corn stalks can produce a large amount of high-concentration D-lactic acid, and the fermentation process is simple, requiring low equipment and easy to promote. It has important industrial application value and development prospects in the industrial field.

Description

technical field [0001] The invention relates to the field of biotechnology and renewable energy, in particular to a method and application for producing D-lactic acid by fermenting straws by Pyrocystis sp. Background technique [0002] Lignocellulose is the main component of straw, and the hydrolysis of lignocellulose is the rate-limiting step in the whole anaerobic digestion, and it is also the difficulty of the whole technology. Lignocellulose is mainly composed of cellulose, hemicellulose and lignin. The covalent bonds combined by lignin and hemicellulose embed cellulose molecules in it. The three-dimensional structure formed by ether bonds and carbon-carbon bonds in lignin has a high Molecular aromatic compounds, these strong bonds inhibit the action of hydrolases. Therefore, lignocellulose needs to be pretreated. The common pretreatment methods of lignocellulose include mechanical method, heat treatment method and chemical treatment, which can effectively promote anae...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/56C12R1/645
CPCC12N1/14C12P7/56C12N1/145C12R2001/645Y02P20/59
Inventor 魏亚琴王治业张静荣
Owner GANSU ACAD OF SCI INST OF BIOLOGY