Detection method of insertion/deletion polymorphism of goat DNAH1 gene and application of detection method

Abstract

The invention discloses a detection method of insertion / deletion polymorphism of a goat DNAH1 gene and an application of the detection method. The DNA of goat complete genome to be detected is used asa formwork, through PCR, a part of fragment of the goat DNAH1 gene is amplified, then agarose gel electrophoresis is performed, and according to the electrophoresis result, the genotype of insertion / deletion polymorphism site of NC_030829.1:g.48594103_48594129 site 27-bp of the goat DNAH1 gene is identified. According to the correlation analysis result, the inventor finds that different genotypesof the 27-bp insertion / deletion polymorphism of the goat DNAH1 gene has significant correlation relationship with lambing number traits of Shanbei white cashmere goats, and a DNA marker capable of increasing goat lambing number trait exists. The method for detecting the insertion / deletion polymorphism of the goat DNAH1 gene provided by the invention can be applied to molecular marker assisted selective breeding of goats, and establishing of good goat heredity resource groups is accelerated.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the 27-bp insertion / deletion polymorphism (InDel) of goat sperm flagella (Multiple morphological abnormalities of the sperm flagella, MMAF) related gene DNAH1NC_030829.1:g.48594103_48594129 Rapid and accurate typing detection of loci and its application in marker-assisted selection (MAS) breeding. Background technique [0002] With the continuous improvement of people's living standards, consumers have higher and higher requirements for the output and quality of livestock and poultry products. The development of animal breeding technology has caused greater changes in the output of livestock and poultry products than in the past, but the growth environment of livestock and poultry often changes , coupled with changes in consumer preferences and palatability, global population growth, etc., animal breeding still faces the challenge of improving new breeds. Traits...

AI Technical Summary

Problems solved by technology

[0004] With the in-depth study of comparative genomics, InDel provides a large amount of biological information for theoretical research and genetic breeding application research. As a genetic identification marker, it mostly focuses on genome research of humans and various crops (such as rice and corn, etc.) Among them, there is little research and application on the functional genes of ruminant livestock, especially the indel markers that can be applied to the breeding of ruminant reproductive traits

However, there is no report on the InDel locus of DNAH1 gene and its significant correlation with litter size traits

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