Application of picrasma quassioides bitter principle compounds in ant killing
A technology of bittern and compound, which is applied to the field of plant-derived bittern-like compound antkiller bait and its preparation, can solve the problems such as no killing of red fire ants, non-target biological damage, water source pollution and the like, and achieves good results. Application prospect, effect of good stomach toxicity activity
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Embodiment 1
[0042] Extraction, Isolation and Structure Identification of Bitterinoids from Tongkat Ali
[0043] Tongkat Ali root 10kg, pulverized into coarse powder, extracted by cold soaking with 95% ethanol, combined extracts, concentrated under reduced pressure to obtain 270g of total extract. Suspend the total extract with water, extract sequentially with petroleum ether, ethyl acetate, and n-butanol, and recover the solvent under reduced pressure respectively to obtain petroleum ether fraction (40g), ethyl acetate fraction (99g), and n-butanol fraction (85g) and water fractions (26g).
[0044] The fractions of petroleum ether were subjected to silica gel column chromatography, chloroform-methanol system gradient elution (100:0-0:100, v / v), combined by TLC analysis, and 7 main fractions (Fr.a-Fr.g) were obtained .
[0045] Fr.c was subjected to ODS column chromatography, methanol-water gradient elution (20:80-100:0, v / v), combined by TLC analysis to obtain Fr.c1-Fr.c4. Among them, ...
Embodiment 2
[0094] Evaluation of the activity of bitterwood picrites against red fire ants
[0095] Preparation of eurycolactone F (compound 12) ant-killing bait and its control effect on red imported fire ants
[0096] (1) Preparation method of 0.1% compound 12 ant-killing bait
[0097] Compound 12 is dissolved with acetone, and the weight percentages of components and each component are as follows:
[0098]
[0099] (2) Preparation method of 1% compound 12 ant-killing bait
[0100] Compound 12 is dissolved with acetone, and the weight percentages of components and each component are as follows:
[0101]
[0102] (3) 10% compound 12 ant-killing bait preparation method
[0103] Compound 12 is dissolved with acetone, and the weight percentages of components and each component are as follows:
[0104]
[0105] Pass the corn flour through a 60-mesh sieve, mix the above-mentioned corn flour, brown sugar and butter respectively, add water to make a dough, squeeze the dough through...
Embodiment 3
[0114] Preparation of eurycolactone E (compound 3) ant-killing bait and its control effect on red imported fire ants
[0115] (1) Preparation method of 0.1% compound 3 ant-killing bait
[0116] Compound 3 is dissolved with acetone, and the weight percentages of components and each component are as follows:
[0117]
[0118] (2) 1% Compound 3 Anticide bait preparation method
[0119] Compound 3 is dissolved with acetone, and the weight percentages of components and each component are as follows:
[0120]
[0121] (3) 10% Compound 3 Anticide bait preparation method
[0122] Compound 3 is dissolved with acetone, and the weight percentages of components and each component are as follows:
[0123]
[0124] Pass the corn flour through a 60-mesh sieve, mix the above-mentioned corn flour, brown sugar and butter respectively, add water to make a dough, squeeze the dough through a 20-mesh sieve to form granules, and dry at 40°C to prepare the filling bait. Take the anticide...
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