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Antagonistic drug against replication of porcine reproductive and respiratory syndrome virus (PRRSV) and application of antagonistic drug

A technology for respiratory syndrome and pig reproduction, applied in antiviral agents, respiratory diseases, drug combinations, etc., can solve problems such as reproductive obstacles in pregnant sows, economic losses in the pig breeding industry, and unsatisfactory commercial vaccines

Active Publication Date: 2019-12-27
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The virus infection can cause reproductive disorders in pregnant sows, respiratory system disorders in pigs of all ages, immunosuppression in pigs, and diseases that promote secondary infections, causing huge economic losses to the pig breeding industry at home and abroad
Commercial vaccines are not yet ideal
The cross-protection ability of the vaccine against strains is not complete, and the process of live virus replication in pigs has the possibility of virus virulence returning to strength and recombining with wild viruses to form new strains
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is an important pathogen that endangers the sustainable and healthy development of the pig industry all over the world, and commercial vaccines are not yet ideal

Method used

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  • Antagonistic drug against replication of porcine reproductive and respiratory syndrome virus (PRRSV) and application of antagonistic drug
  • Antagonistic drug against replication of porcine reproductive and respiratory syndrome virus (PRRSV) and application of antagonistic drug
  • Antagonistic drug against replication of porcine reproductive and respiratory syndrome virus (PRRSV) and application of antagonistic drug

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Experimental program
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Embodiment Construction

[0043] 1. Materials and methods

[0044] 1.1 Cells and viruses

[0045] Cells of highly pathogenic PRRSV strain BB0907, classic PRRSV strain S1, and NADC30-like strains FJ1402 and Marc145 were kept in the laboratory; porcine alveolar macrophages (PAM) were isolated from 4-6 week-old PRRSV, PCV2 and PRV-negative piglets. The PRRSV strain BB0907, classic PRRSV strain S1, and NADC30-like strain FJ1402 and Marc145 cells involved in this experiment are all conventional strains disclosed in the prior art, and the isolation method of porcine alveolar macrophages (PAM) is also Conventional methods already disclosed in the prior art.

[0046] 1.2 Main reagents

[0047] Xanthohumol for in vitro experiments was purchased from Selleck Chemicals with a purity of >99%; xanthohumol for in vivo experiments was purchased from Chengdu Ruifensi Biotechnology Company with a purity of >98%; protoporphyrin IX cobalt chloride (CoPP ) is an inducer of HMOX1 expression, which was purchased from Si...

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Abstract

The invention discloses an antagonistic drug against replication of a porcine reproductive and respiratory syndrome virus (PRRSV) and application of the antagonistic drug. A virus infection test is adopted, and Xanthohumol (Xn), the antagonistic drug of the PRRSV, is discovered for the first time from a natural medicine library with 386 plant sources. The drug can effectively inhibit PRRSV replication in both Marc-145 and PAM cells, five xanthohumol derivatives with different molecular structures are artificially synthesized, and the viral infection test further finds that the inhibition effect to virus replication by drug derivative Xn-4 is highest in vitro; and test results of artificial infection and drug therapy in piglets show that the Xn-4 can effectively inhibit PRRSV viremia, relieves clinical symptoms of infected pigs, and significantly reduces pulmonary inflammation and pathological damage. Combined with the results, it is proved that the antagonistic drug has important preventive and therapeutic effects to a porcine reproductive and respiratory syndrome, and has important application prospects.

Description

technical field [0001] The invention belongs to the technical field of veterinary prevention and control drugs, in particular to a new drug for antagonizing the replication of porcine reproductive and respiratory syndrome virus and its application. Background technique [0002] The pathogen of porcine reproductive and respiratory syndrome is porcine reproductive and respiratory syndrome virus (porcine reproductive and respiratory syndrome virus, PRRSV), which belongs to Arteriviridae and is a single-stranded positive-sense RNA virus. The virus infection can cause reproductive disorders in pregnant sows, respiratory system disorders in pigs of all ages, immunosuppression in pigs, and diseases that promote secondary infections, causing huge economic losses to the pig breeding industry at home and abroad. Commercial vaccines are not yet ideal. The cross-protection ability of the vaccine against the virus strains is not complete, and the process of live virus replication in pig...

Claims

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Application Information

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IPC IPC(8): A61K31/12A61P31/14A61P11/00C07C45/74C07C49/84
CPCA61K31/12A61P31/14A61P11/00C07C45/74C07C49/84C07C45/64C07C45/455C07C49/825
Inventor 姜平刘雪威白娟张军忍王先炜李玉峰
Owner NANJING AGRICULTURAL UNIVERSITY
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