Cordyceps militaris cultivated by using hermetia illucens pupa as host and cultivation method thereof
A breeding method and technology for water flies, which are applied in the directions of microorganism-based methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problem of low infection rigidity and sloppy rate, unevenness, and short fruiting bodies of Cordyceps militaris. and other problems, to achieve the effect of reducing pathogenic microorganisms, low cost, rigid and stable infection
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Embodiment 1
[0044] This embodiment includes the following steps:
[0045] (1) Select healthy black soldier fly larvae in the middle and late stages of the sixth instar. Under the conditions of temperature 25° C. and relative humidity of 60% to ensure ventilation, feed the larvae with artificial feed of the same weight continuously for 2 days, then fast for 2 days, and select robust black soldier fly larvae for standby;
[0046] The artificial feed ingredients are: 30g of mulberry leaf powder, 40g of soybean meal powder, 20g of corn flour, 2.5g of multivitamins, 2.5g of citric acid, 2.5g of minerals and 2.5g of other ingredients such as preservatives;
[0047] (2) Take the robust black soldier fly larvae obtained in step (1), and inject the diluted OD into each head 600 0.8 strain filtrate 0.15ml, put the worm body on the bamboo sieve plaque immediately after the injection is completed, then cultivate 3 days at a temperature of 20°C, a relative humidity of 60%, and ventilation conditions;...
Embodiment 2
[0051] This embodiment includes the following steps:
[0052] (1) Select six instar 3d healthy black soldier fly larvae, at a temperature of 28° C., relative humidity of 65%, and ensure ventilation, feed artificial feeds of equal weight to larvae daily and fast for 2 days, and select healthy black soldier fly larvae for use; ;
[0053] The artificial feed mentioned above: mulberry leaf powder 10g, soybean meal powder 50g, corn flour 30g, multivitamin 2.5g, citric acid 2.5g, mineral matter 2.5g and preservative 2.5g.
[0054] (2) Select the robust black soldier fly larvae obtained in step (1), inject each head with diluted OD 600 1.0 Cordyceps strain filtrate 0.10ml strain filtrate, put the worm body on the bamboo sieve plaque immediately after the injection is completed, then cultivate 3d at a temperature of 23°C, a relative humidity of 70%, and ventilation conditions; Continue culturing for 8 days at 24°C, with a relative humidity of 70% and under ventilated conditions. Af...
Embodiment 3
[0058] This embodiment includes the following steps:
[0059] (1) Select healthy black soldier fly larvae in the middle and late stages of the sixth instar. Under the condition of temperature 28°C, relative humidity 65%, and ensure ventilation, feed the larvae with artificial feed of equal weight every day and then fast for 2 days. Select robust black soldier fly larvae for backup. ;
[0060] The artificial feed mentioned above: 20g of mulberry leaf powder, 40g of soybean meal powder, 30g of corn flour, 2.5g of multivitamins, 2.5g of citric acid, 2.5g of minerals and 2.5g of preservatives.
[0061] (2) Select the strong and clean black soldier fly larvae obtained in step (1), and inject each head with diluted OD 600 0.6 Cordyceps strain filtrate 0.015ml strain filtrate, put the worm body on the bamboo sieve plaque immediately after the injection is completed, then cultivate 3d at a temperature of 25°C, a relative humidity of 70%, and ventilated conditions; then cover it with...
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