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Calcium chelating peptide as well as preparation method and application thereof

A calcium chelating and preparation type technology is applied in the field of calcium chelating peptide and its preparation, which can solve the problems of low processing utilization rate and the like, and achieve the effects of improving absorption and bioavailability, improving physical and chemical activity, and promoting calcium transport.

Active Publication Date: 2019-12-31
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

my country is rich in seafood resources. For example, the output and export volume of tilapia rank among the top in the world, and its processing utilization rate is relatively low due to the industrial structure. The leftovers produced during the processing are good raw materials for the preparation of calcium chelating peptides. source

Method used

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  • Calcium chelating peptide as well as preparation method and application thereof
  • Calcium chelating peptide as well as preparation method and application thereof
  • Calcium chelating peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] This embodiment provides a calcium chelating peptide whose amino acid sequence is: FDHIVY (793.3888Da).

[0053] The calcium chelating peptide can be prepared by the following preparation method:

[0054] (1) Extraction of tilapia bone collagen

[0055] (1) Cleaning of tilapia skeleton raw materials

[0056] After the fish skeleton is thawed, impurities are removed and cleaned with tap water, and finally the fish skeleton is decapitated.

[0057] (2) De-meat treatment of tilapia skeleton

[0058] Cut the fish skeleton into about 5 cm, take 1 kg of raw material, add 5 g of neutral protease and 1000 mL of water, and carry out enzymatic hydrolysis on the fish skeleton for 2 hours (55°C, pH 7.5) to remove the minced meat attached to the bone. Impurities are washed away after enzymatic hydrolysis.

[0059] (3) Alkaline Elution Degreasing of Tilapia Bones

[0060] Put the fish bones into 0.1mol / L NaOH solution (w / v1:10) and stir for 24h, and wash until neutral.

[0061]...

Embodiment 2

[0132] In this example, the calcium chelating ability of the calcium chelating peptide and its effect on the calcium transport of the Caco-2 cell monolayer were studied, and the specific process was as follows.

[0133] (1) Peptide synthesis

[0134] Synthetic peptides (FDHIVY) with a purity higher than 95% were obtained from Synpeptide Co., Ltd (Nanjing, China), and their molecular weight was determined by LC / ESI-MS, which was 793.3888 Da.

[0135] Table 4 shows the homologous proteins of calcium chelating peptides.

[0136] Table 4 Homologous tissues and proteins of calcium chelating peptides

[0137]

[0138] The calcium sequestration activity of the synthetic peptides was determined by using the o-cresolphthalein colorimetric method. Such as Figure 8 , the calcium chelating activity of FDHIVY was 35.73 mg / g, indicating that the shorter peptide FDHIVY has more calcium chelating sites.

[0139] Interestingly, some peptide sequences with high affinity for calcium were...

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Abstract

The invention relates to a calcium chelating peptide as well as a preparation method and an application thereof. An amino acid sequence of the calcium chelating peptide is FDHIVY. The calcium chelating peptide provided by the invention is safe and non-toxic, and has better physicochemical activity compared with a traditional calcium supplement, and the calcium chelating capability of the calcium chelating peptide reaches 35.73 mg / g; the collagen peptide can be supplemented while absorption and bioavailability of calcium in a human body are improved, the absorption and transport of calcium in Caco-2 small intestinal epithelial cells can be promoted, and the calcium chelating peptide can be used as a raw material for drug development and biological calcium supplementation agents. According to the preparation method provided by the invention, the calcium chelating peptide is successfully obtained, fish product resources can be fully utilized, the operation is simple, and a new way is provided for high-valued utilization of tilapia bones.

Description

technical field [0001] The invention belongs to the technical field of preparation of functional health food, and in particular relates to a calcium chelating peptide and its preparation method and application. Background technique [0002] Calcium is the main component of bone minerals. The human body obtains calcium mainly through food intake. Studies have found that the imbalance of calcium metabolism caused by insufficient calcium intake is the cause of many diseases that occur in people of all ages. At present, calcium insufficiency has become a worldwide nutritional phenomenon. The daily recommended calcium intake for adults is generally 800mg / d. Recommended intake requirements. [0003] Calcium ions can form a complex with a ring structure with peptides, which has high stability, and can make calcium ions be absorbed and utilized in the form of chelates, improving its bioavailability, and is also conducive to the release of calcium ions. It is a good choice as a bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/20A23L33/165A23L33/18A23L2/52A61K38/39A61P3/02
CPCC07K14/78C12P21/06A23L33/165A23L33/18A23L2/52A61P3/02A23V2002/00A61K38/00A23V2200/30A23V2250/55A23V2250/1578
Inventor 苗建银廖婉雯曹庸
Owner SOUTH CHINA AGRI UNIV
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