Magnetic molecularly imprinted nano-composite-based electrochemical sensor, preparation method thereof and application of electrochemical sensor

A molecular imprinting and electrochemical technology, which is applied in the direction of material electrochemical variables, scientific instruments, and material analysis through electromagnetic means, can solve the problems of strict storage conditions, expensive enzymes, cumbersome pretreatment, etc., and achieve large adsorption capacity, The effect of low raw material cost and fast mass transfer rate

Active Publication Date: 2019-12-31
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, chromatography instruments are expensive, pretreatment is cumbersome, and professional operators are required, while in immunoassays, enzymes are expensive, stored under strict conditions, and easily inactivated

Method used

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  • Magnetic molecularly imprinted nano-composite-based electrochemical sensor, preparation method thereof and application of electrochemical sensor
  • Magnetic molecularly imprinted nano-composite-based electrochemical sensor, preparation method thereof and application of electrochemical sensor
  • Magnetic molecularly imprinted nano-composite-based electrochemical sensor, preparation method thereof and application of electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Fe 3 o 4 NPs were purchased from Nanjing Zhongkebeier Biotechnology Co., Ltd. Bisphenol A was purchased from aladdin company. Example 1 PDA@Fe 3 o 4 Synthesis of MIP MNPs

[0030] 1. First, add 10mg Fe 3 o 4 NPs (purchased from Nanjing Zhongkebaier Biotechnology Co., Ltd., 50mg / mL, ethanol dispersion) were dispersed in 4mL Tris-HCl (10mM, pH8. 0), repeat 3 times to remove the residual ethanol solution on the surface. The template molecule bisphenol A (0.05 mmol) was dissolved in 1 mL of acetonitrile, and added dropwise to Fe 3 o 4 In the Tris-HCl solution of NPs, after the dropwise addition, ultrasonication for 1 min and mechanical stirring for 60 min were performed to pre-assemble the bisphenol A on Fe 3 o 4 NPs aggregated on the surface to obtain a pre-assembled solution.

[0031] 2. Add dopamine hydrochloride (0.075 mmol) into the pre-assembly solution while stirring, sonicate for 10 s, continue mechanical stirring, and react at room temperature for 3 h....

Embodiment 2

[0034] Embodiment 2PDA@Fe 3 o 4 Synthesis of MIP MNPs

[0035] 1. First, add 10mg Fe 3 o 4 NPs (purchased from Nanjing Zhongkebaier Biotechnology Co., Ltd., 50mg / mL, ethanol dispersion) were dispersed in 4mL Tris-HCl (15mM, pH8. 5), repeat 3 times to remove the ethanol solution residue on the surface. The template molecule bisphenol A (0.1 mmol) was dissolved in 1 mL of acetonitrile and added dropwise to Fe 3 o 4 In the Tris-HCl solution of NPs, after the dropwise addition, ultrasonication for 1 min and mechanical stirring for 60 min were performed to pre-assemble the bisphenol A on Fe 3 o 4 NPs aggregated on the surface to obtain a pre-assembled solution.

[0036] 2. Add dopamine hydrochloride (0.015 mmol) into the pre-assembly solution while stirring, sonicate for 10 s, continue mechanical stirring, and react at room temperature for 3 h. After the reaction, the black product was separated with a magnet and washed 2 to 3 times with deionized water to remove unreacte...

Embodiment 3

[0038] Example 3 PDA@Fe 3 o 4 Synthesis of MIP MNPs

[0039] 1. First, add 10mg Fe 3 o 4 NPs (purchased from Nanjing Zhongkebaier Biotechnology Co., Ltd., 50mg / mL, ethanol dispersion) were dispersed in 4mL Tris-HCl (20mM, pH 9.0 ), repeat 3 times to remove the residual ethanol solution on the surface. The template molecule bisphenol A (0.15mmol) was dissolved in 0.25mL acetonitrile, and added dropwise to Fe 3 o 4 In the Tris-HCl solution of NPs, after the dropwise addition, ultrasonication for 1 min and mechanical stirring for 60 min were performed to pre-assemble the bisphenol A on Fe 3 o 4 NPs aggregated on the surface to obtain a pre-assembled solution.

[0040] 2. Add dopamine hydrochloride (0.1 mmol) into the pre-assembly solution while stirring, sonicate for 10 s, continue mechanical stirring, and react at room temperature for 3 h. After the reaction, the black product was separated with a magnet and washed 2 to 3 times with deionized water to remove unreacted ...

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Abstract

The invention discloses a polydopamine coated magnetic Fe3O4 molecularly imprinted polymer, a preparation method thereof and application of the polydopamine coated magnetic Fe3O4 molecularly imprintedpolymer. The invention also discloses an electrochemical sensor based on the polydopamine-coated magnetic Fe3O4 molecularly imprinted polymer, a preparation method of the electrochemical sensor and application of the electrochemical sensor. According to the polydopamine coated magnetic Fe3O4 molecularly imprinted polymer, the preparation method thereof, the application of the polydopamine coatedmagnetic Fe3O4 molecularly imprinted polymer, the electrochemical sensor based on the polydopamine-coated magnetic Fe3O4 molecularly imprinted polymer, the preparation method of the electrochemical sensor and the application of the electrochemical sensor of the invention, Fe3O4 is used as a carrier, molecular imprinting is directly carried out on the surface of the Fe3O4, and therefore, a complexsurface modification process is simplified. DDT recognition, extraction and separation performed a target compound have the advantages of specificity, simple steps, high efficiency and the like; a surface molecular imprinting technology is combined with electrochemical impedance spectroscopy (EIS), so that the constructed electrochemical impedance sensor integrates the advantage of selectivity ofthe molecular imprinting and the advantage of high sensitivity of the EIS.

Description

technical field [0001] The invention belongs to the technical field of molecularly imprinted electrochemical sensors, and in particular relates to an electrochemical sensor based on magnetic molecularly imprinted nanocomposites, a preparation method thereof and a detection of organochlorine pesticide DDT. Background technique [0002] Organochlorine pesticides are high-efficiency broad-spectrum insecticides, which have been widely used all over the world due to their good insecticidal and infectious disease prevention effects. Organochlorine pesticides are potentially toxic, stable and difficult to degrade, which makes them accumulate in large quantities in the environment and enter organisms through the food chain, posing a threat to the ecological environment and human health. Therefore, it is of great significance to establish a fast and effective sample pretreatment method and a stable and sensitive analysis and detection method. [0003] The detection methods of DDT re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 刘安然苗娇娜於明珠卫伟刘松琴
Owner SOUTHEAST UNIV
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