Micro-fluidic magneto-sensitive immune device based on magnetoresistive biosensor and using method thereof

A biosensor and microfluidic technology, applied in biological testing, measuring devices, instruments, etc., can solve the problems of complex operation and cumbersome steps, and achieve the effect of simplifying operation steps, solving cumbersome steps, and improving the efficiency of biochemical reactions

Active Publication Date: 2019-12-31
XI AN JIAOTONG UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

The microfluidic device designed by it injects the sample to be tested, immunomagnetic beads and washing solution into the reaction area step by step. The immunomagnetic beads used need to be pre-marked before they can be used, resulting in cumbersome steps and complicated operations.

Method used

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  • Micro-fluidic magneto-sensitive immune device based on magnetoresistive biosensor and using method thereof
  • Micro-fluidic magneto-sensitive immune device based on magnetoresistive biosensor and using method thereof
  • Micro-fluidic magneto-sensitive immune device based on magnetoresistive biosensor and using method thereof

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Embodiment Construction

[0037] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0038] refer to figure 1 , the present invention proposes a microfluidic magneto-sensitive immune device based on a magnetoresistive biosensor, including an upper cover 1 , a microfluidic chip 2 , a magnetic sensor array 3 and a bottom plate 4 . The upper cover 1 is buckled on the microfluidic chip 2, and the two are connected by gluing or mechanical fixing.

[0039] see figure 2The upper cover 1 includes a housing 11, and the housing 11 is provided with four reagent storage areas, namely a superparamagnetic bead storage area 12, an antibody storage area 13, a sample storage area 14 and a washing solution storage area 15. The superparamagnetic bead storage area 12, the antibody storage area 13, the sample storage area 14 to be tested and the washing solution storage area 15 are all stepped...

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Abstract

The invention discloses a micro-fluidic magneto-sensitive immune device based on a magnetoresistive biosensor and a using method thereof. In the micro-fluidic magneto-sensitive immune device, a superparamagnetic bead storage area and an antibody storage area are arranged on an upper cover; a micro-fluidic chip is provided with a first biochemical reaction cavity and a first micro-fluidic channel;in use, the biotinylated superparamagnetic beads and the biotinylated antibodies are sequentially subjected to mixed reaction in the first biochemical reaction cavity and the first microfluidic channel, the reacted superparamagnetic beads marked with the antibodies react with a to-be-detected sample to generate immune protein marked with the superparamagnetic beads, and then the immune protein isreacted and combined with capture antibodies in the magnetic sensor array, so that the superparamagnetic beads are fixed on sensors in the magnetic sensor array for a subsequent detection process. Thecombination of the superparamagnetic beads and the biotinylated antibody in the magneto-sensitive immunoassay is completed in the chip, so that the integration level is improved, the operation stepsof biochemical reaction are simplified, and the biochemical reaction efficiency is improved.

Description

technical field [0001] The invention relates to magneto-sensitivity immune analysis, in particular to a microfluidic magneto-sensitivity immune device based on a magnetoresistance biosensor and a method for using the same. Background technique [0002] Magnetic sensitivity immunoassay is a new immunoassay method after fluorescence immunoassay, chemiluminescence, electrochemiluminescence, electrochemistry, and surface Raman enhancement. It is an indirect quantitative method by detecting and analyzing the magnetic signal of the antigen labeled with magnetic beads Compared with other immunoassay methods, magnetic sensitivity immunoassay is easy to operate, low in cost, and has extremely high sensitivity. Magnetic sensors are important devices for magneto-sensitivity immunoassays. Commonly used are Hall sensors, magnetoresistance sensors (AMR), giant magnetoresistance sensors (GMR), tunneling magnetoresistance sensors (TMR) and giant magnetoresistance sensors (GMI), etc. . Due...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/74G01N33/53G01N33/532G01N33/543G01N27/06
CPCG01N27/06G01N27/745G01N33/5304G01N33/532G01N33/54326
Inventor 彭年才张航胡飞李治鹏郭晓牛
Owner XI AN JIAOTONG UNIV
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