Rifampicin drug-resistant tubercle bacillus detection method, device and equipment, and storage medium

Abstract

The invention provides a rifampicin drug-resistant tubercle bacillus detection method, device and equipment, and a storage medium. The method comprises the steps of obtaining literature information related to tubercle bacillus whole genome sequencing; extracting a whole genome sequencing data serial number and rifampicin drug resistance mutation information from the literature information, and downloading whole genome sequencing data according to the whole genome sequencing data serial number; processing the whole genome sequencing data to extract fixed mutation; and constructing a detection system evolution tree based on a mutation site of the fixed mutation and corresponding rifampicin drug-resistant mutation information so as to identify compensatory mutation. In the invention, whetherclinical rifampicin drug-resistant tubercle bacillus carries the related compensatory mutation can be detected in an accelerated manner so that an evolution stage of the drug-resistant tubercle bacillus is judged, and guidance is provided for clinical medication. Along with further accumulation of global tubercle bacillus sequencing data, detection accuracy of the method, device, equipment and medium is further improved in turn.

Description

technical field [0001] The invention relates to the technical field of drug resistance detection, in particular to a detection method, device, equipment and storage medium for rifampicin-resistant tuberculosis. Background technique [0002] Tuberculosis is an ancient disease caused by infection with the bacterium Mycobacterium tuberculosis, but it is still the number one infectious disease in the world today. Mycobacterium tuberculosis acquires drug resistance mainly through point mutations in drug-resistant genes, and the targets of antibiotics often play an important role in the growth of Mycobacterium tuberculosis. These drug-resistant mutations generally lead to changes in the secondary structure of their encoded products. In an environment without antibiotics, compared with sensitive bacteria, drug-resistant tuberculosis bacteria will show characteristics such as slower growth, smaller colonies, and decreased virulence, which is called "adaptive cost". However, drug-re...

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Problems solved by technology

Due to the clear mechanism of action of rifampicin, concentrated drug-resistant mutation sites, and clear associated genes, studies on its compensatory mutations are common; recent studies have shown that tuberculosis bacteria that accumulate rifampicin-resistant mutations are often also resistant to isoniazid (rifampicin). Fuping and isoniazid are the most commonly used first-line anti-tuberculosis drugs) drug resistance. If effective treatment is not received at this time, tuberculosis patients will gradually face the embarrassing situation of no available drugs

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