Supercharge Your Innovation With Domain-Expert AI Agents!

Application of recombination salmonella VNP20009-M to preparation of drugs for treating osteosarcoma

A technology of VNP20009-M and Salmonella, applied in the field of tumor biology, can solve the problems of immune response, 5-year survival rate of OS patients not exceeding 20%, side effects, etc., achieve the effect of inhibiting proliferation and good development and application prospects

Inactive Publication Date: 2020-01-03
GUANGDONG UNIV OF TECH
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the 1970s, amputation was the most common method of treatment for OS, but the 5-year survival rate of OS patients treated with this method has never exceeded 20%
However, since mammals themselves do not express methionase, the way of exogenous administration has certain side effects, often causing the body's immune response

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of recombination salmonella VNP20009-M to preparation of drugs for treating osteosarcoma
  • Application of recombination salmonella VNP20009-M to preparation of drugs for treating osteosarcoma
  • Application of recombination salmonella VNP20009-M to preparation of drugs for treating osteosarcoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The induction effect of VNP20009-M on osteosarcoma cell apoptosis detected by flow cytometry

[0018] 1) Take the MNNG-HOS osteosarcoma cells in the logarithmic growth phase, and use 2*10 5 cells / ml inoculated in 6-well culture plate, 2ml per well;

[0019] 2) After the plates are spread, add the bacteria at the experimental concentration, the cell ratio is 1:50, and set the PBS control group and the bacteria control group at the same time, and continue to culture the cells for 4 hours;

[0020] 3) After 4 hours, cells were harvested, washed twice with cold PBS, and prepared into 1×10 with 1*BindingBuffer 6 cells / mL cell suspension, take 100μl in the flow tube, add 5μL 7AA-D and 5μL PE staining according to the kit instructions, gently vortex the cells, incubate at room temperature in the dark for 15min, then add 400uL*BindingBuffer to the tube , within 1h for flow cytometry, FlowJoV10 analysis software analysis results.

[0021] The result is as figure 1 Shown: VNP...

Embodiment 2

[0023] After U2OS, Saos-2, and MNNG-HOS overexpressed methioninase, the cell migration ability was detected.

[0024] 1) Take the cells MNNG-HOS, U2OS, and Saos-2 after the transfection of the plasmid to prepare a single-cell suspension, and use 2*10 cells per well. 5 Each / 200ul was inoculated on the transwell chamber. Be divided into 3 groups altogether: complete control group A, plasmid transfection control group B, plasmid transfection group C;

[0025] 2) A moderate amount of 20% FBS acts as a chemoattractant in the lower chamber. Remove non-invasive cells with a cotton swab. Invading cells were stained with crystal violet, and microscopy images were taken to quantify invading cells.

[0026] The result is as figure 2 Shown: The migration ability of osteosarcoma cells was significantly reduced. CON is the complete control group, EGFP is the plasmid control group, and MEGL is the plasmid group (experimental group) carrying the methionase gene.

Embodiment 3

[0028] CCK8 method was used to detect the effect of overexpression of methionase gene on the proliferation of osteosarcoma cells.

[0029] 1) Take the MNNG-HOS cells in the logarithmic growth phase to prepare a single cell suspension. 2ml of cell suspension per well (containing 2×10 5 cells) were inoculated in a 6-well plate, and were divided into 3 groups: complete control group A, plasmid transfection control group B, and plasmid transfection group C;

[0030] 2) After the plates were spread, liposomes, a mixture of liposomes and a control plasmid, and a mixture of liposomes and a plasmid carrying methionase were added to each group, and the cells were further cultured for 24 hours;

[0031]3) Take the cells MNNG-HOS transfected with the plasmid to prepare a single cell suspension, and use 100ul of cell suspension per well (containing 2×10 3 cells) were inoculated in a 96-well plate, and were divided into three groups: complete control group A, plasmid transfection control...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of genetically engineered bacterium VNP20009-M to preparation of drugs for treating osteosarcoma, and belongs to the field of oncobiology. The application comprises apoptosis inducing, and cell proliferation and migration inhibiting. According to the application, attenuated salmonella typhimurium is used as a carrier to carry methionine enzyme genes with the purpose of specifically consuming methionine in the osteosarcoma to inhibit the growth of the osteosarcoma. The engineered bacterium VNP20009-M can significantly inhibit the proliferation of tumor cellsand the growth of the osteosarcoma in the body, it is predicted that the engineered bacterium VNP20009-M can be an important target in the study of anti osteosarcoma proliferation, a basis is provided for the preparation of anti osteosarcoma proliferation drugs, good prospects of development and application are achieved, and potential clinical application value is achieved. The administration mode of the genetically engineered bacterium is preferably intratumoral injection.

Description

technical field [0001] The invention relates to the application of a genetically engineered bacterium VNP20009-M in the preparation of medicines for treating osteosarcoma, including inducing cell apoptosis and inhibiting cell proliferation and migration, belonging to the field of tumor biology. Background technique [0002] Osteosarcoma (OS) is one of the most common malignant bone tumors. Its incidence rate ranks first among primary bone tumors. It occurs most frequently in adolescents aged 0 to 24, followed by the elderly. Among them, the incidence rate and The age is relatively stable, while the incidence and age of onset of the elderly vary greatly. Before the 1970s, amputation was the most common method for treating OS, but the 5-year survival rate of OS patients treated with this method has not exceeded 20%. With the advancement of surgical techniques, the introduction of the concept of neoadjuvant chemotherapy, and the improvement of postoperative radiotherapy and ch...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/74A61P35/00A61P35/04
CPCA61K35/74A61P35/00A61P35/04Y02A50/30
Inventor 赵子建张馨丹李芳红赵正刚李美蓉萧耿苗
Owner GUANGDONG UNIV OF TECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com