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Lactobacillus reuteri capable of relieving atopic asthma Th2 reaction and application of Lactobacillus reuteri

A technology of Lactobacillus reuteri and medicinal excipients, applied in the fields of microorganisms and medicine, can solve the problems of unclear effect relationship, poor compliance, slow effect, etc.

Active Publication Date: 2020-01-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, since most bronchodilator drugs and anti-inflammatory drugs need to be administered by intravenous injection or inhalation, the patient's compliance with these drugs is poor, which leads to repeated attacks of the patient's disease, and over time it will cause the patient's Severe complications such as emphysema and pulmonary heart disease
[0006] Although probiotic preparations are relatively safe and healthy, the currently found probiotics such as Lactobacillus adderiformis, Lactobacillus rhamnosus and Bifidobacterium animalis that have been found to alleviate the symptoms of allergic asthma may have poor or unclear effects. , therefore, most of the probiotic preparations on the market also have the defect of slow effect, or have the defect of unclear action relationship
[0007] Therefore, there is an urgent need to find a probiotic that reduces the Th2 response of allergic asthma and has a strong ability to relieve allergic asthma, so as to solve the problems that the existing probiotic preparations for allergic asthma are slow to take effect and the relationship between the effects is not clear

Method used

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  • Lactobacillus reuteri capable of relieving atopic asthma Th2 reaction and application of Lactobacillus reuteri
  • Lactobacillus reuteri capable of relieving atopic asthma Th2 reaction and application of Lactobacillus reuteri
  • Lactobacillus reuteri capable of relieving atopic asthma Th2 reaction and application of Lactobacillus reuteri

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: Screening and identification of Lactobacillus reuteri

[0048] Specific steps are as follows:

[0049] 1. Screening:

[0050] Taking human feces from Laizhou, Shandong as the sample, the sample was pretreated and stored in a -80°C refrigerator in about 30% glycerin. After taking out and thawing, mix the sample and add 0.5mL sample to 4.5mL, then add 0.9 Perform gradient dilution with % normal saline, select the appropriate gradient dilution and spread it on the MRS solid medium, culture at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS solid medium, pick a single colony and transfer to MRS The liquid medium was enriched and preserved in 30% glycerol to obtain strain CCFM1072.

[0051] 2. Identification:

[0052] The 16S rDNA of CCFM1072 was amplified and sequenced, and the nucleotide sequence of the sequence was compared in NCBI. The result showed that the strain was Lactobacillus reuteri, and was named as Lactobacillus reuteri...

Embodiment 2

[0053] Embodiment 2: the cultivation of Lactobacillus reuteri

[0054] Specific steps are as follows:

[0055] Lactobacillus reuteri (Lactobacillus reuteri) CCFM1072 was inserted into the MRS solid medium and cultured at 37°C for 48 hours, and its colonies were observed under a microscope. It was found that the colonies were milky white, irregular and round Raised, smooth, slightly irregular-shaped, rounded-ended campylobacters, usually present singly, in pairs, and in small clusters.

[0056] Insert Lactobacillus reuteri (Lactobacillus reuteri) CCFM1072 into MRS liquid medium and culture at 37°C for 18 hours, then transfer to fresh MRS liquid medium, and cultivate under the same conditions for 18 hours, centrifuge the bacteria at 6000g for 15 minutes, and add 0.9% normal saline After washing, the cells were centrifuged again at 6000 g for 10 min to obtain cells, which were resuspended in 30% sucrose solution and frozen at -80°C until use.

Embodiment 3

[0057] Example 3: Effect of Lactobacillus reuteri on pulmonary inflammation in mice with allergic asthma

[0058] Specific steps are as follows:

[0059] The 6-week-old SPF grade BALB / c female mice were divided into 5 groups, namely the normal group, the model group and the experimental group. The L.reuteri1 prevention group of Lactobacillus reuteri 1 and the L.reuteri2 prevention group of Lactobacillus reuteri 2 by oral administration, 6 animals in each group, were raised in the Experimental Animal Center of Jiangnan University, fed with common feed, and kept at a constant temperature of 21-26°C. The humidity is 40-70%, the noise is less than or equal to 60dB, and the animal illumination is 15-20LX (all animal experiment procedures are reviewed and approved by the Animal Welfare and Ethics Management Committee of Jiangnan University).

[0060] The experiment lasted for 6 weeks (5 days a week), and the model was established in the 2nd to 6th weeks. The mice in the model group...

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Abstract

The invention discloses Lactobacillus reuteri capable of relieving atopic asthma Th2 reaction and application of Lactobacillus reuteri and belongs to the technical fields of microorganisms and medicine. The Lactobacillus reuteri has an atopic asthma relieving function, specifically reflected as follows: (1) significantly reducing lung inflammation reaction of atopic asthma mice; (2) remarkably inhibiting production of total immunoglobulin IgE in serum of the atopic asthma mice; (3) significantly decreasing the content of IL-5 in lungs of atopic asthma mice; (4) significantly decreasing the content of IL-13 in lungs of atopic asthma mice; and (5) significantly raising the content of propionic acid in intestines of atopic asthma mice. Therefore, the Lactobacillus reuteri has great application prospects in preparation of a product for preventing and / or treating atopic asthma.

Description

technical field [0001] The invention relates to a strain of Lactobacillus reuteri capable of alleviating the Th2 response of allergic asthma and its application, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Allergic asthma is a common clinical disease, difficult to treat, and the course of the disease is protracted and difficult to heal. Mediators amplify the inflammatory cascade, increasing airway hyperresponsiveness (AHR) and stimulating mucus secretion, leading to airway obstruction and airway remodeling. In susceptible individuals, this inflammation can cause recurrent episodes of wheezing, shortness of breath, chest tightness, and / or coughing, which can lead to life-threatening complications such as respiratory arrest and respiratory failure. [0003] Asthma In developing countries, the prevalence of asthma is increasing year by year. Its pathogenesis is affected by many factors, such as exposure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23L2/39A23L33/135A61K35/747A61P11/06C12R1/225
CPCC12N1/20A23C9/1234A23L2/39A23L33/135A61K35/747A61P11/06A23V2002/00C12R2001/225C12N1/205A23V2400/173A23V2200/314A23V2250/5114
Inventor 陈卫陆文伟李凌智翟齐啸张秋香赵建新杭锋张灏
Owner JIANGNAN UNIV
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