Method for detecting ribavirin and metabolites thereof in livestock and poultry hairs

A ribavirin and detection method technology, which is applied in the field of detection of ribavirin and its metabolites in livestock and poultry hair, can solve the problems of low recovery rate, time-consuming, difficult storage, etc., and achieve lower detection limit, The effect of improving detection sensitivity and improving recovery rate

Active Publication Date: 2020-01-07
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, the technical problem to be solved in the present invention is to overcome ribavirin and TCONH in the prior art 2 To detect the problems of inconvenient sampling, diff

Method used

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  • Method for detecting ribavirin and metabolites thereof in livestock and poultry hairs
  • Method for detecting ribavirin and metabolites thereof in livestock and poultry hairs
  • Method for detecting ribavirin and metabolites thereof in livestock and poultry hairs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Conditional investigation experiment:

[0038] 1. Configuration of standard solution:

[0039] Standard stock solution: accurately weigh appropriate amount of ribavirin, TCONH 2 and 13 C 5 - Ribavirin standard products were prepared into standard stock solutions of 1.0 mg / mL with methanol, and stored at -20°C in the dark.

[0040] Standard intermediate solution: Pipette an appropriate amount of standard stock solution into a 10mL volumetric flask, dilute with methanol to obtain a standard intermediate solution with a mass concentration of 10.0mg / L, and store at -20°C in the dark.

[0041] Standard working solution: Pipette an appropriate amount of standard intermediate solution into a 10mL volumetric flask, dilute with methanol to a standard working solution with a mass concentration of 1.0mg / L, and store at -20°C in the dark.

[0042] 2. Optimization of sample processing method conditions

[0043] (1) Selection of hydrolysis solvent

[0044] The dense structure o...

Embodiment 2

[0082] This embodiment provides a method for detecting ribavirin and its metabolites in chicken feathers, the specific steps are:

[0083] 1. Sample preparation Take 5 positive chicken feather samples and 5 blank chicken feather samples obtained by oral ribavirin administration and feeding. Among them, the specific way of administration and feeding is that 5 broilers in the experimental group are fed with ribavirin raw powder aqueous solution according to the standard of 10 mg / kg body weight, and fed continuously for 3 days, and 5 broilers in the blank group are replaced with equal amount of normal saline for ribavirin Linyuan powder aqueous solution, hair samples were collected after the experiment for testing.

[0084] 2. Preparation of the test solution

[0085] (1) Hair cleaning: wash the hair with 1% SDS for 30 minutes, then rinse it with distilled water for 3 times until it is clean, dry the sample in an oven at 45°C, then cut it into pieces of 1-2 mm, and then grind it...

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Abstract

The invention, which belongs to the technical field of drug residue detection, particularly relates to a method for detecting ribavirin and metabolites thereof in livestock and poultry hairs. The method comprises the following steps: homogeneously extracting ribavirin and metabolites thereof remained in a sample by using a methanol solution of formic acid; dispersing and purifying an extracting solution by using an ethylenediamine-N-propylsilane (PSA) matrix and an octadecylsilane (C18) matrix; and then carrying out ultra-high performance liquid chromatography tandem mass spectrometry detection. Internal standard method quantification is carried out. The mobile phase A is a 0.1% (v/v) ammonium formate and ammonium acetateaqueous solution; the mobile phase B is methanol; the flow velocity is 0.4 mL/min; and the chromatographic column employs an SB-Aq polar column with dimensions of 1.8 micrometers and 3mm*100mm. According to the invention, defects that sampling is difficult, living bodyslaughtering is needed and the like are overcome. The method has high specificity, high sensitivity and short consumed time based on optimization of a sample treatment step; and the sample purification requirement is low. With the provided method, a reliable technical support is provided for supervision of livestock and poultry product quality safety.

Description

technical field [0001] The invention belongs to the technical field of drug residue detection, and in particular relates to a method for detecting ribavirin and its metabolites in livestock and poultry hair. Background technique [0002] Ribavirin is a broad-spectrum antiviral drug for humans. It is mostly used clinically to treat Lassa fever, infant respiratory syncytial virus pneumonia, influenza A, B, and hepatitis C (Khatami, 2014). [0003] The exposure of the "fast-growing chicken" incident in 2012 showed that the phenomenon of "human medicine and veterinary use" of ribavirin in animal breeding is extremely common. Although my country’s Announcement No. 560 issued in 2005 explicitly prohibited ribavirin and other human antiviral drugs from being transplanted into animals, there is currently a lack of effective veterinary antiviral drugs, and farmers have limited options. Ribavirin has good efficacy and low price. In order to reduce the risk of breeding and pursue the m...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/72
CPCG01N30/02G01N30/34G01N30/72
Inventor 徐俊谢敏周瑶敏闵佳玲邬磊万伟杰胡丽芳许晓红肖勇
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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