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Bacillus halophilus for improving salt resistance of nile tilapia and application of bacillus halophilus

A technology of halophilic Bacillus and Nile tilapia, applied in the field of halophilic Bacillus and halophilic Bacillus, can solve the problems of affecting the absorption of nutrients, hidden dangers of food safety, poor management and other problems, so as to improve digestion and absorption ability, anti-oxidative ability, and antibacterial ability

Inactive Publication Date: 2020-01-10
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, it was usually used to increase the frequency of water changes, and a large number of antibiotics were used only for the purpose of high yield and disease prevention, but it would have a considerable impact on the quality of aquatic products and seriously affected the economic value of Nile tilapia
[0003] Under the conditions of intensive, large-scale and environmental changes, animals will have stress responses due to stocking density, virus infection, fright, etc., and various adverse physiological reactions will occur, which will affect the absorption of nutrients, and then affect the reduction of growth performance, giving The production efficiency of the farm caused huge losses
There are not only factors such as poor management, but also overuse of antibiotics that leads to drug resistance of pathogenic bacteria, and chemical drug residues that interfere with the intestinal flora of aquatic animals and continue to damage the ecological environment, affecting the health of aquatic animals, resulting in excessive drug content in aquatic products , the quality of aquatic products has declined, bringing huge hidden dangers to food safety issues

Method used

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  • Bacillus halophilus for improving salt resistance of nile tilapia and application of bacillus halophilus
  • Bacillus halophilus for improving salt resistance of nile tilapia and application of bacillus halophilus
  • Bacillus halophilus for improving salt resistance of nile tilapia and application of bacillus halophilus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take some intestines of Nile tilapia, grind the sample to make it homogenate, and dilute it with sterile phosphate buffer solution to a concentration of 10 -1 The suspension was serially diluted 10 times, and 200 μL of suspensions with different dilutions were absorbed, spread on ordinary seawater medium, and cultured at 30°C (aerobic or anaerobic) for 3 days. After picking out colonies with different traits for staining and microscopic examination, further purification and culture were carried out; preservation and culture were carried out. The purified strain is inoculated in ordinary seawater culture medium, cultured overnight at 200 r / min at 30°C for 20-24 hours to obtain the bacterial liquid.

[0020] Detection of hemolytic activity: Purify the bacterial liquid, and streak culture on ordinary seawater agar medium. Pick the isolated and purified single colony, plant it on a blood plate with a sterile toothpick, and observe whether there is a hemolytic ring around t...

Embodiment 2

[0037] The influence of halophilic bacillus 221 of the present invention added on the tilapia basal feed on the growth performance of tilapia:

[0038] The experiment selects 100 tilapias domesticated through 14 days and 200 juvenile tilapias with similar body weight (1.8g ± 0.03g) through 16‰ salt water, and distributes them randomly and evenly in 4 and 8 100L plastic buckets. Wherein the fresh water control group and the 16‰ salinity control group were fed the basal ration, and the 16‰ salinity experimental group was fed the feed of the present invention adding halophilic bacillus 221. All groups were fed at 7:00 in the morning and 17:00 in the afternoon. After 56 days of the experiment, 15 fish were randomly selected from each group to measure their body length and weight, and their intestines and liver and pancreas were taken to test the antioxidant capacity indicators. The results See Table 2 and Table 3.

[0039] Table 2 The influence of halophilic bacillus 221 of the p...

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Abstract

The invention discloses bacillus halophilus for improving the salt resistance of nile tilapia and application of the bacillus halophilus. An ordinary seawater liquid medium of the bacillus halophilusis activated under the aseptic condition; activated bacterial liquid is subjected to enlarging cultivation, and a cultured culture solution is an inoculating solution; and after twice cleaning with sterilized phosphoric acid buffer solution, resuspension is conducted to prepare a cell suspension, the cell suspension is sprayed evenly on basal feed of the nile tilapia, and thus the concentration ofthe feed is increased to 108 cfu / g. According to the bacillus halophilus and the application thereof, the bacillus halophilus 221 is added to the basal feed of the nile tilapia, the digestibility andabsorption ability of the nile tilapia living in the environment with salinity being 1.6% is significantly improved, the antioxidant ability and the antibacterial ability can be improved, and a favorable foundation is laid for the provided quality of the tilapia; and meanwhile, great significance to improve the compound feed of the tilapia is also achieved.

Description

technical field [0001] The invention belongs to the field of biological technology and relates to a halophilic bacillus, in particular to a halophilic bacillus improving the salt tolerance of Nile tilapia and an application thereof. Background technique [0002] my country's aquaculture industry has developed rapidly, and the total amount of aquaculture has ranked first in the world for many years. Among all cultured fish, tilapia is the most important freshwater fish in the world. Nile tilapia is an economic fish in my country and an excellent aquaculture species with development potential. With the expansion of intensive farming scale and the change of breeding environment, higher requirements are put forward for its growth performance and disease prevention. In the past, the frequency of water change was usually improved, and a large number of antibiotics were used for the purpose of high yield and disease prevention, but this would have a considerable impact on the qual...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/80C12R1/07
CPCA23K10/18A23K50/80C12N1/20C12N1/205C12R2001/07
Inventor 汤上上刘树彬王世锋李二超
Owner HAINAN UNIVERSITY
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