Bacillus halophilus for improving salt resistance of nile tilapia and application of bacillus halophilus
A technology of halophilic Bacillus and Nile tilapia, applied in the field of halophilic Bacillus and halophilic Bacillus, can solve the problems of affecting the absorption of nutrients, hidden dangers of food safety, poor management and other problems, so as to improve digestion and absorption ability, anti-oxidative ability, and antibacterial ability
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Embodiment 1
[0019] Take some intestines of Nile tilapia, grind the sample to make it homogenate, and dilute it with sterile phosphate buffer solution to a concentration of 10 -1 The suspension was serially diluted 10 times, and 200 μL of suspensions with different dilutions were absorbed, spread on ordinary seawater medium, and cultured at 30°C (aerobic or anaerobic) for 3 days. After picking out colonies with different traits for staining and microscopic examination, further purification and culture were carried out; preservation and culture were carried out. The purified strain is inoculated in ordinary seawater culture medium, cultured overnight at 200 r / min at 30°C for 20-24 hours to obtain the bacterial liquid.
[0020] Detection of hemolytic activity: Purify the bacterial liquid, and streak culture on ordinary seawater agar medium. Pick the isolated and purified single colony, plant it on a blood plate with a sterile toothpick, and observe whether there is a hemolytic ring around t...
Embodiment 2
[0037] The influence of halophilic bacillus 221 of the present invention added on the tilapia basal feed on the growth performance of tilapia:
[0038] The experiment selects 100 tilapias domesticated through 14 days and 200 juvenile tilapias with similar body weight (1.8g ± 0.03g) through 16‰ salt water, and distributes them randomly and evenly in 4 and 8 100L plastic buckets. Wherein the fresh water control group and the 16‰ salinity control group were fed the basal ration, and the 16‰ salinity experimental group was fed the feed of the present invention adding halophilic bacillus 221. All groups were fed at 7:00 in the morning and 17:00 in the afternoon. After 56 days of the experiment, 15 fish were randomly selected from each group to measure their body length and weight, and their intestines and liver and pancreas were taken to test the antioxidant capacity indicators. The results See Table 2 and Table 3.
[0039] Table 2 The influence of halophilic bacillus 221 of the p...
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