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Method for improving lignocellulose saccharification efficiency

A technology of lignocellulose and saccharification efficiency, applied in the field of biotransformation, can solve the problems of low saccharification efficiency and low saccharification efficiency, and achieve the effect of increasing accessibility

Pending Publication Date: 2020-01-10
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the use of cellulase to saccharify lignocellulosic materials has the disadvantage of low saccharification efficiency. The main reason for the low saccharification efficiency is that the hemicellulose and lignin in lignocellulosic materials limit the access of cellulase to the cellulose substrate.

Method used

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  • Method for improving lignocellulose saccharification efficiency
  • Method for improving lignocellulose saccharification efficiency
  • Method for improving lignocellulose saccharification efficiency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The method for improving the saccharification efficiency of lignocellulose includes the steps:

[0032] (1) Pretreatment: pretreatment of rice straw, the specific process is,

[0033] (1.1) Select rice straw, clean, dry, crush, sieving, and store in a drying box;

[0034] (1.2) Accurately weigh 20g rice straw powder, put it into a 500mL Erlenmeyer flask, add 93.5mL absolute ethanol and 46.5mL nitric acid, place it in a 76℃ water bath, and react for 3h under normal pressure;

[0035] (1.3) After the reaction is completed, filter the solid with 500 mesh filter cloth, and filter with vacuum pump. Part of the lignin component in the rice straw is nitrated by nitric acid, and the resulting nitrated lignin is dissolved in ethanol solution and washed continuously with deionized water Until the filtrate is clarified to remove solubles and residual nitric acid and ethanol;

[0036] (1.4) Collect the remaining solids, put them in an oven and bake them until the weight no longer changes, g...

Embodiment 2

[0044] The method for improving the saccharification efficiency of lignocellulose includes the steps:

[0045] (1) Pretreatment: pretreatment of rice straw, the specific process is,

[0046] (1.1) Cut corn stalks and crush them to powder;

[0047] (1.2) Weigh 100g of powdered corn stalks, add 1000ml of deionized water, add 30g of sodium chlorite and 10ml of glacial acetic acid, place in the reactor, heat to 75°C, add 30g of sodium chlorite and 10ml of glacial acetic acid every 10min. React for 40 minutes to obtain a mixture.

[0048] (1.3) After the reaction is completed, filter the filter residue with a 500 mesh filter cloth, and continuously wash the filter residue with deionized water until the filtrate is clarified to obtain delignified corn stover.

[0049] (2) Enzymatic hydrolysis: add cellulase, enzymatic hydrolysis additives, and solvents to the delignified corn stalks for enzymatic saccharification. The specific process is to add 50 mg of cellulase (1u / mg) to the delignified c...

Embodiment 3

[0052] The method for improving the saccharification efficiency of lignocellulose includes the steps:

[0053] (1) Pretreatment: pretreatment of rice straw, the specific process is,

[0054] (1.1) Cut corn stalks and crush them to powder;

[0055] (1.2) Weigh 40g of powdered corn stalks, add 400ml of deionized water, add 12g of sodium chlorite and 4ml of glacial acetic acid, place in the reactor, heat to 75℃, add 12g of sodium sulfite and 4ml of glacial acetic acid every 10min, and react for 20min to obtain mixture;

[0056] (1.3) After the reaction is completed, filter the filter residue with a 500 mesh filter cloth, and continuously wash the filter residue with deionized water until the filtrate is clarified to obtain delignified corn stalks. The delignified corn stalk is chemically pretreated with Phanerochaete chrysosporium Nutrients required for the enzyme production medium, sterilized at 121°C and inoculated with Phanerochaete chrysosporium for pretreatment;

[0057] (1.4) After...

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Abstract

The invention relates to the field of biotransformation, in particular to a method for improving lignocellulose saccharification efficiency. The invention relates to the method for improving lignocellulose saccharification efficiency, which comprises the following steps: (1) pretreating a lignocellulose material: pretreating the lignocellulose material to obtain a pretreated product, and pulverizing the pretreated product into powder; (2) performing enzymolysis: adding cellulase, an enzymolysis additive and a solvent into the pretreated product to carry out enzymatic saccharification reaction;and (3) obtaining a lignocellulose saccharification product: after an enzymatic saccharification reaction is finished, obtaining a centrifuged supernate which is the lignocellulose saccharification product. According to the method, the accessibility of hydrolysis factors to cellulose is improved by pretreating the lignocellulose material and adding an enzymolysis additive, so that the saccharification efficiency of the lignocellulose is improved.

Description

Technical field [0001] The invention relates to the field of biotransformation, in particular to a method for improving the saccharification efficiency of lignocellulose. Background technique [0002] Lignocellulosic material is the most abundant and lowest cost biomass material in the world. It is a kind of supramolecular functional structural polymer which is held together by complex cellulose chains through hydrogen bonds. It is composed of carbohydrate polymers (cellulose and semi- A mixture of cellulose), lignin, pectin and ash. The supramolecular structure of lignocellulose is complex. It is a heterogeneous polymer formed by the combination of cellulose, hemicellulose and lignin. In addition to hydrogen bonds, lignin and cellulose are also connected by covalent bonds. The crystalline and amorphous phases of cellulose coexist in a tightly arranged three-dimensional linear structure, and lignin forms an amorphous, nonlinear and uneven three-dimensional structure. Polymeric s...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02D21C3/16D21C3/20
CPCC12P19/14C12P19/02C13K1/02D21C3/16D21C3/20C12P2201/00
Inventor 江正兵宋慧婷李华南肖文静
Owner HUBEI UNIV
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