Droplet generating system for digital PCR detection and digital PCR detecting method

Abstract

The invention discloses a droplet generating system for digital PCR detection and a digital PCR detecting method. The droplet generating system comprises a micro pipeline, a rotating driving mechanism, a fluid driving mechanism and a digital PCR chip, wherein the micro pipeline is provided with a first opening and a second opening allowing liquid to enter and exit; the rotating driving mechanism is used for driving the micro pipeline to perform reciprocating swinging; the fluid driving mechanism is used for driving liquid to pass through the micro pipeline; the digital PCR chip comprises a chip body with a droplet storing cavity, a liquid inlet formed in the chip body, an accommodating cavity which is vertically formed in the chip body and communicates with the liquid inlet, a liquid discharging opening formed in the chip body, a first channel for communicating between the liquid inlet and the droplet storing cavity, and a second channel for communicating between the liquid dischargingopening and the droplet storing cavity; and the end, where the first opening is located, of the micro pipeline can be inserted into the accommodating cavity, and can be in reciprocating swinging in the accommodating cavity under the driving of the rotating driving mechanism. The droplet generating system disclosed by the invention integrates the functions of droplet formation and droplet detection, so that more notable and accurate detecting results can be obtained.

Description

technical field [0001] The invention relates to a liquid drop generation system and a digital PCR detection method for digital PCR detection. Background technique [0002] Polymerase chain reaction (PCR) technology is one of the most important tools in modern biology. It is widely used in medical diagnosis, personalized medicine, food inspection, genetically modified organism detection, pathogen identification, immune analysis, forensic science, etc. As the latest generation of PCR technology, digital PCR (dPCR) based on the development of microfluidic technology has smaller reaction volume, faster reaction speed, lower system noise and higher sensitivity than traditional qPCR. [0003] Droplet Digital PCR (Droplet Digital PCR) technology is a water-in-oil droplet technology based on microfluidic chips. It encapsulates a single DNA molecule into a single droplet through a water-in-oil structure, and uses the inertness of oil to realize DNA molecules. Each DNA molecule is co...

Images