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Trichoderma guizhouense and application thereof

A technology of Trichoderma and mycelium, used in fungi, fungicides, chemicals for biological control, etc., can solve problems such as plant health threats, excessive heavy metals, and soil fertility decline.

Active Publication Date: 2020-01-14
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Plant diseases caused by plant pathogenic bacteria pose a great threat to plant health, causing crop yield reduction or even failure
Although chemical control has always been the main measure to prevent and control plant diseases, the long-term use of chemical pesticides has caused serious decline in soil fertility, excessive heavy metals, and damage to ecological balance.

Method used

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  • Trichoderma guizhouense and application thereof
  • Trichoderma guizhouense and application thereof
  • Trichoderma guizhouense and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the isolation, purification and identification of Trichoderma strain

[0044] 1. Isolation and Purification of Trichoderma

[0045] Take soil samples from the forest area of ​​Milin County, Nyingchi Prefecture, Tibet, weigh 1g of soil samples, and dilute to 10 with sterile water -1 、10 -2 and 10 -3 Three concentration gradients, each concentration gradient takes 200 μl and spreads on PDA medium (potato 200g, glucose 20g, agar 20g, distilled water to 1000ml, sterilized at 121 ℃ and added to the medium at a final concentration of 300mg / l chloramphenicol) on the surface of a 9cm diameter petri dish. After inverting the plate and incubating at 25°C for 1–3 days, pick a single colony and transfer it to PDA slant medium.

[0046] 2. Species identification of Trichoderma strains

[0047] Biological features: see figure 1 , on CMD medium, after 3 days of culture at 25 ° C, the colony radius is 70–73 mm, and the plate is full in 3 days. The colonies are gr...

Embodiment 2

[0066] Embodiment 2, the application of Trichoderma

[0067] 1. Confrontation with plant pathogenic bacteria

[0068] Guizhou Trichoderma TC952 and Botrytis cinerea (Zhao Juan, Liu Ting, Liu Weicheng, et al. were selected from the preserved slant medium respectively. Screening and identification of Streptomyces against Botrytis cinerea [J]. Microbiology Bulletin, 2019,46 (10): 2548-2558), Fusarium graminearum (the fungus is stored in the China Agricultural Microorganism Culture Collection Center, the preservation number is: ACCC 31053) and Rhizoctonia solani (Rhizoctonia solani ACCC 36124) 3 plant pathogenic bacteria in Activate on the PDA medium, then use a 5mm diameter puncher to cut the bacterial block at the edge of the activated colony as the inoculum, and inoculate it on a 9cm petri dish containing PDA. The inoculum of TC952 and plant pathogenic bacteria are separated on the PDA plate 5 cm, and a plate inoculated with only plant pathogenic bacteria at one end was used a...

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Abstract

The invention discloses a trichoderma guizhouense TC952 and application thereof. The trichoderma guizhouense TC952 is strong in environmental adaptability, high in growth speed and rich in conidia production, and has obvious growth inhibition effects on Botrytis cinerea, Fusarium graminearum and Rhizoctonia solani with inhibition rates of 78.8%, 100%, and 100%, respectively, through confrontationculture on plates. The trichoderma guizhouense TC952 can hyperparasitize on the three plant pathogenic bacteria and effectively kill the pathogenic bacteria by winding and invading hyphae of the plantpathogenic bacteria. The trichoderma guizhouense TC952 is suitable for effective control of crop diseases caused by the above pathogenic bacteria, e.g., the trichoderma guizhouense TC952 can controlcucumber rhizoctonia rot caused by rhizoctonia solani with a control effect of 52.9%, and has a good and stable control effect.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases, and in particular relates to a Trichoderma guizhou strain and application thereof. Background technique [0002] Plant diseases caused by phytopathogenic bacteria pose a great threat to plant health and cause crop yield reduction or even failure. Although chemical control has always been the main measure to prevent and control plant diseases, the long-term use of chemical pesticides has caused serious decline in soil fertility, excessive heavy metals, and damage to ecological balance. Therefore, using biological control, the strategy of "using bacteria to control bacteria" is widely recognized and has become a research hotspot at home and abroad. [0003] Trichoderma has the characteristics of fast growth, wide distribution and strong environmental adaptability, and has antagonistic effects on many plant pathogens. Since Weindling first discovered in 1932 that Trichoderma lig...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01P3/00C12R1/885
CPCC12N1/14C12N1/145C12R2001/885
Inventor 庄文颖张意陈凯
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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