Application of serum endogenous small molecules in evaluating differentiation degree of laryngeal cancer tumor cells

A tumor cell, endogenous technology, applied in the application field of serum endogenous small molecules in evaluating the differentiation degree of laryngeal cancer tumor cells, can solve the problem of lack of accurate quantitative evaluation methods for the degree of tumor differentiation, and achieve less damage to the body , low cost, enhanced objectivity and accuracy

Inactive Publication Date: 2020-01-14
FIRST HOSPITAL OF SHANXI MEDICAL UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to provide an effective serum metabolomics method including normalized values ​​of fingerprints and differential metabolite integral areas, and use this quantitative value as an indicator of tumor differentiation degree to solve the problem of lack of accurate quantitative evaluation methods for tumor differentiation degree

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  • Application of serum endogenous small molecules in evaluating differentiation degree of laryngeal cancer tumor cells
  • Application of serum endogenous small molecules in evaluating differentiation degree of laryngeal cancer tumor cells
  • Application of serum endogenous small molecules in evaluating differentiation degree of laryngeal cancer tumor cells

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Embodiment Construction

[0032] The screening method for biomarkers of laryngeal cancer comprises the following steps:

[0033] 1. Sample preparation:

[0034] Serum samples were thawed in ice-water mixture, took 100 μL and added 400 μL acetonitrile, shaken well, centrifuged at 13000 rpm for 20 min at 4 °C, took the supernatant and dried, added 100 μL 80% acetonitrile-water (v / v), vortexed 2min, centrifuge at 13000rpm for 20min at 4°C, take the supernatant for LC-MS analysis;

[0035] 2. LC-MS analysis test conditions:

[0036] Liquid phase conditions: mobile phase A, water containing 0.1% formic acid; mobile phase B, acetonitrile containing 0.1% formic acid. Gradient elution: 0-2min, 2% B; 2-13min, 2% B-35% B; 13-28min, 35% B-98% B; 28-30min, 98% B; 30-32min, 98% B-2%B; 32-34min, 2%B. Injection volume: 3μL; flow rate, 0.2mL / min; column temperature: 45°C; WatersACQUITYUPLC HSST3 column (2.1mm×100mm, 1.8μm).

[0037] Mass spectrometry conditions: HESI ionization method; spray voltage: positive ele...

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Abstract

The invention belongs to the technical field of serum endogenous small molecule application, and particularly relates to an application of serum endogenous small molecules in evaluating a differentiation degree of laryngeal cancer tumor cells. According to the application of the serum endogenous small molecules in evaluating the differentiation degree of the laryngeal cancer tumor cells, the serumendogenous small molecules refer to eight laryngeal cancer biological metabolism markers, which are screened out by means of performing LC-MS metabonomics analysis on the basis of a laryngeal squamous cell carcinoma serum sample; and the eight laryngeal cancer biological metabolism markers specifically include a metabolite L-Carnitine, a metabolite 9-Decenolicarnitine, a metabolite Glycocholic acid, a metabolite Oleoyl glycinine, a metabolite cis-5-Tetronecenolicarnitine, a metabolite 3, 5-Tetradecadiencarnitine, a metabolite 2-Hydroxyadipic acid and a metabolite Sphingosine 1-phosphate, andthe differentiation degree of the laryngeal cancer tumor cells is evaluated according to content changes of the eight serum endogenous small molecules. According to the laryngeal cancer differentiation degree evaluation method based on serum metabonomics, a serum sample is adopted, the cost is low, and damage to the body is small.

Description

technical field [0001] The invention belongs to the technical field of application of serum endogenous small molecules, and in particular relates to the application of serum endogenous small molecules in evaluating the degree of differentiation of laryngeal cancer tumor cells. Background technique [0002] Laryngeal cancer is the most common malignant tumor of the larynx, and squamous cell carcinoma is the most common type, and its incidence is high in northern China. Clinically, the different degrees of malignancy of laryngeal cancer have different effects on the body, and the degree of malignancy of the tumor can be predicted by the degree of differentiation. The degree of tissue differentiation is high, and the structural atypia of cells and tissues is small; the degree of differentiation is low, the cell atypia is more obvious, the growth is rapid, the infiltration destroys the structure and function of the organ, and metastasis can occur. Generally, squamous cell carci...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 崔佳佳李震宇高伟吴勇延杨东丽牛敏张宇良胡汪来王斌全崔永萍李会政薄云峰郑希望胡英还李文奇石勇桑江玮李俊刘红亮
Owner FIRST HOSPITAL OF SHANXI MEDICAL UNIV
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