Polyvalent antibody and preparation method thereof

Abstract

The embodiment of the invention discloses a polyvalent antibody and a preparation method thereof. The polyvalent antibody includes a plurality of antigen-binding sites, wherein the antigen-binding sites are connected to a human-source antibody core fragment; and the antigen-binding sites at least include antigen-binding sites specifically bound with tumor cells and antigen-binding sites specifically bound with immunocyte. The polyvalent antibody is obtained through reformation in a genetic engineering manner, and can enable the immunocyte to be bound to the cancer cells to arouse attack of theimmunocyte on the cancer cells. Compared with a conventional CAR-T treatment method, a treatment method disclosed by the invention has the advantages that the polyvalent antibody has broad spectrum,does not depend on autologous T cells of patients, and can be in mass production. In addition, waiting time does not exist, and the polyvalent antibody can be immediately prepared for use. Relying ona mature antibody production technology, risk infected by other pathogens can be eliminated in the manner of GMP and the like, and the polyvalent antibody has lower treatment fees.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a multivalent antibody and a preparation method thereof. Background technique [0002] Traditional treatments for cancer include surgery, radiation, chemotherapy, and targeted therapy. However, these traditional treatment methods have limitations to varying degrees, and can only delay but cannot fundamentally prevent the development of the disease. [0003] For example, surgical treatment is only suitable for tumor tissues that are visible to the naked eye. Radiotherapy and chemotherapy can kill a large number of tumor cells, but they are not specific enough and will destroy a large number of normal tissue cells and produce strong side effects. Although targeted drugs have obvious therapeutic effects and quick results, they are prone to drug resistance and heterogeneity of tumor cells, and cannot exert long-term anti-tumor effects. [0004] In order to overcome the limitations of t...

AI Technical Summary

Problems solved by technology

[0010] In view of the above technical problems, the embodiment of the present invention provides a multivalent antibody and its preparation method to solve the problems of a series of defects in the existing tumor immunotherapy methods, which cannot be well promoted and applied

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