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Method for analyzing differential chemical components of seeds of Ziziphus jujuba Mill. and seeds of Ziziphus mauritiana Lam. through UHPLC-Q-Orbitrap MS

A technology of chemical composition and jujube kernel, applied in the field of high-resolution mass spectrometry analysis of the difference chemical composition of jujube kernel and jujube kernel, which can solve the problems of confusion in clinical application and disparity in price.

Active Publication Date: 2020-01-21
汉方萃取生物科技(海南)股份有限公司
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  • Application Information

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Problems solved by technology

Due to the similar characteristics and traditional efficacy of jujube kernels and jujube kernels, the prices of the two are very different, resulting in the phenomenon that a large number of jujube kernels are sold as jujube kernels, causing confusion in the market and clinical application of the two.

Method used

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  • Method for analyzing differential chemical components of seeds of Ziziphus jujuba Mill. and seeds of Ziziphus mauritiana Lam. through UHPLC-Q-Orbitrap MS
  • Method for analyzing differential chemical components of seeds of Ziziphus jujuba Mill. and seeds of Ziziphus mauritiana Lam. through UHPLC-Q-Orbitrap MS
  • Method for analyzing differential chemical components of seeds of Ziziphus jujuba Mill. and seeds of Ziziphus mauritiana Lam. through UHPLC-Q-Orbitrap MS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: establish Semen Semen Semen Semen Jujube and Semen Semen Semen UHPLC-Q-Orbitrap-MS analysis method, comprising the steps:

[0031] Step (1) Collection of samples. The details are as follows: The wild jujube kernels used in the experiment were identified by Associate Professor Du Chenhui of Shanxi University of Traditional Chinese Medicine as the wild jujube plant of Rhamnaceae Ziziphus jujuba Mill. var. spinosa (Bunge) Dried mature seeds of Hu ex H. F. Chou; Ziziphus mauritiana Dry ripe seeds of Lam. A total of 16 batches of jujube kernels and 13 batches of jujube kernel samples were collected and stored in the Modern Research Center of Traditional Chinese Medicine of Shanxi University. See Table 1 for details.

[0032] Table 1 Information sheet of 29 batches of samples

[0033]

[0034] Step (2) Preparation of reference substances. Purchased 24 reference substances from Baoji Chenguang Biotechnology Co., Ltd., Sichuan Weikeqi Biotechnology Co....

Embodiment 2

[0042] Embodiment 2: adopt the chemometrics method to analyze Semen Semen Semen Semen Semen Semen Semen Semen Jujube and Semen Semen Semen Jujube, comprising the following steps:

[0043] Step (1) Inspection of instrument precision. Specifically as follows: in the embodiment 1 step (6), the Excel table is obtained, and the peak area matrix of 16 jujube kernels, 13 jujube kernels, and 10 QC samples in the table is imported into Simca-p (13.0) software, and principal component analysis is carried out (principal component analysis, PCA). Such as figure 2 As shown, in the positive and negative ion modes, the QC samples are closely gathered together, indicating that the instrument has good precision during the experiment.

[0044] Step (2) PCA analysis. The details are as follows: Import the peak area matrices of 16 jujube kernels and 13 jujube kernel samples in the Excel table in step (6) of Example 1 into Simca-p (13.0) software for principal component analysis. The results ...

Embodiment 3

[0046] Embodiment 3: the identification of differential metabolites, comprises the steps:

[0047]The details are as follows: For compounds with reference substances, identify the compounds according to the retention time, precursor ion and secondary fragment information; for compounds without reference substances, firstly identify the compound by comparing the parent ion and secondary fragment information of the compound with the literature, and then refer to Compound Discover software matching results. Using Compound Discover software, an Excel table including molecular weight, retention time, peak area, and compounds matched to the online database can be obtained. A total of 40 differential metabolites, 2 saponins, 19 flavonoids and 19 alkaloid components were identified from 431 differential variables according to this method, as shown in Table 2.

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Abstract

The invention belongs to the technical fields of analysis and quality control of traditional Chinese medicines, and provides a method for analyzing differential chemical components of seeds of Ziziphus jujuba Mill. and seeds of Ziziphus mauritiana Lam. through UHPLC-Q-Orbitrap MS. The method comprises the following steps: Soxhlet extraction is carried out on medicinal material powders of the seedsof Ziziphus jujuba Mill. and the seeds of Ziziphus mauritiana Lam. through petroleum ether for degreasing, 70% ethyl alcohol is added for heating reflux extraction, and sample solutions are obtainedafter filtering; chemical component analysis is carried out on the sample solutions through UHPLC-Q-Orbitrap MS, and peak matching, peak alignment, denoising and normalization processing is carried out on mass spectrum data through software Compound Discover to obtain an Excel table containing molecular weights, retention times and peak areas of compounds; and a compound peak area data matrix is imported into software Simca-p for multivariate statistical analysis, which is combined with an ROC curve to find and identify differential metabolites of the seeds of Ziziphus jujuba Mill. and the seeds of Ziziphus mauritiana Lam.. The method provided by the invention has the advantages that material bases of the seeds of Ziziphus jujuba Mill. and the seeds of Ziziphus mauritiana Lam. are disclosed, so that reference for the establishment of a new traditional Chinese medicine quality evaluation system can be provided.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine analysis and quality control of traditional Chinese medicine, and specifically relates to a method for analyzing the difference chemical components of Semen Semen Semen Semen Semen Semen Semen Jujube and Semen Semen Jujube by using UHPLC-Q-Orbitrap MS. method of chemical composition. Background technique [0002] Ziziphus jujuba Ziziphus jujuba Mill. var. spinosa (Bunge) The dried and mature seeds of Hu ex H. F. Chou, Lizaoren is the same plant of the same family as Suanzioren, Diancizao Ziziphus mauritiana The mature seeds of Lam. are commonly called "Dianzaoren", "Myanmar jujube kernel" or "imported jujube kernel". Both of them have the effects of calming the mind, calming the nerves and suppressing sweat in the traditional effect. The 2015 edition of "Chinese Pharmacopoeia" records that Suanzaoren is "sweet, sour, and flat; it belongs to the liver, gallbladder, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/72G01N30/86
CPCG01N30/7266G01N30/8679
Inventor 闫艳申晨曦杜晨晖李震宇秦雪梅
Owner 汉方萃取生物科技(海南)股份有限公司
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