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Application of copper-based three-dimensional metal organic framework material to enzyme simulation

A metal-organic framework, three-dimensional technology, applied in the field of biomimetic enzyme sensor preparation, can solve the problems of high price, poor electron transport performance, general catalytic performance, etc., and achieve the effect of good catalytic performance

Inactive Publication Date: 2020-01-24
NANJING UNIV OF SCI & TECH
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  • Abstract
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Problems solved by technology

[0004] Aiming at the disadvantages of the existing peroxide-mimicking enzyme materials, such as high price, poor electron transport performance, and general catalytic performance, the present invention provides a peroxide-mimicking enzyme material based on a copper-based three-dimensional metal-organic framework, which has monovalent Copper and divalent copper bivalent active sites can efficiently catalyze hydrogen peroxide and have strong electron transport capabilities

Method used

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  • Application of copper-based three-dimensional metal organic framework material to enzyme simulation
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  • Application of copper-based three-dimensional metal organic framework material to enzyme simulation

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Embodiment 1

[0031] The method for preparing the copper-based three-dimensional metal organic framework material (Cu-MOF Ns) of the present invention comprises the following steps:

[0032] Step 1. Dissolve 0.3g of polyvinylpyrrolidone (PVP) in 4mL of DMF (N,N-dimethylformamide) and 4mL of ethanol mixed solution, sonicate for 30±5min, dissolve 24.2mg of Cu(NO 3 ) 2 3(H 2 O) (copper nitrate trihydrate) and 5.4 mg NH 2 -BTC (2-aminoterephthalic acid) was added to 4mL of DMF, and then this solution was mixed with the previously dissolved PVP solution, and they were ultrasonically treated together for 30±5min. The mixture was placed in a 20mL hydrothermal kettle and reacted at 120±5°C for 8±1h.

[0033] Step 2, the product is centrifuged for 5 minutes using a high-speed centrifuge at 13000 rpm.

[0034] Step 3, wash three times with deionized water, freeze and dry in vacuum at low temperature for 8±2 hours, and obtain the product Cu-MOF Ns.

[0035] Other test procedures are the same as s...

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Abstract

The invention discloses an application of a copper-based three-dimensional metal organic framework material to enzyme simulation. The organic framework material is Cu-MOF, and the organic framework material can be used as a biological simulation enzyme sensor for detecting H2O2. An electrocatalytic test is carried out on the Cu-MOF, and it is found that the material can catalyze hydrogen peroxideunder the condition of simulating the neutral acid-base pH of a human body, and the material has a strong catalytic effect.

Description

technical field [0001] The invention belongs to the technical field of preparation of biological imitation enzyme sensors, and relates to the application of a copper-based three-dimensional metal-organic framework. Background technique [0002] In the field of biotechnology, rapid and accurate determination of hydrogen peroxide (H 2 o 2 ) are in increasing demand, and to develop hydrogen peroxide sensors with high sensitivity, excellent selectivity, and good accuracy, various approaches have been used. Over the past decades, electrochemical biosensors based on natural enzymes have been increasingly studied due to their high sensitivity and excellent selectivity. However, electrochemical biosensors based on natural enzymes are usually complex, expensive and highly dependent on external pH and temperature. Therefore, the construction of biosensors that mimic enzymes is a hot spot and focus of current research. Recently, transition metals, transition metal oxides or hydroxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48G01N31/10C08G83/00B01J31/22B82Y40/00
CPCC08G83/008B01J31/1691B01J31/2217B01J37/0018B82Y40/00G01N27/308G01N27/48G01N31/10B01J2531/16B01J35/23B01J35/33
Inventor 单丹刘子萱
Owner NANJING UNIV OF SCI & TECH
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