Fermentation system for simulating colonic environment and simulated fermentation method of system

Abstract

The invention relates to a fermentation system for simulating a colonic environment and a simulated fermentation method of the system and belongs to the fields of bionics, human physiology, microbiology and organic chemistry. The fermentation system comprises an intestinal flora colonization system, a pH control system, a gas path system, a flow rate control system and a temperature and stirring control system. A fermentation method for simulating a colonic environment is characterized by comprising the three main work contents: control parameter setting, intestinal flora colonization and simulated fermentation. By using the fermentation system for simulating the colon of a human body and a simulated experiment method based on the system, the fermentation process for simulating parts of microorganisms in the colon of the human body under an in-vitro condition is realized. The fermentation system is realistic in simulation and has good parallelity, stability and repeatability.

Description

technical field [0001] The invention belongs to the fields of bionics, human physiology, microbiology and organic chemistry. The invention relates to a simulated colonic environment fermentation system and a simulated fermentation method based on the system, in particular to a method for simulating colonic environment and simulating fermentation by colonizing fecal bacteria under in vitro conditions and replacing different improved culture media. Background technique [0002] Because animal experiments will cause great pain to the tested animals, scientists from all over the world are trying to make various colonic fermentation simulators to replace animal experiments, mainly including the reading model, the principle of which is to add sterile medium to represent the proximal colon (pH 5.5) in container 1, and added sequentially to containers 2 and 3 to simulate the transverse colon (pH 6.2) and distal colon (pH 6.8), respectively. The pH was controlled by an external pH c...

AI Technical Summary

Problems solved by technology

The PolyFermS model, the principle is that the fresh fecal microbiota is collected in the gel beads, and the bacteria are released from the peripheral layer before entering the fermentation medium to multiply to achieve high density and long stable fermentation time. The advantage of this new method is that in Up to 80 days of operation time significantly improves the bacterial density of the system and increases the stability of the system, the disadvantage is that the main limitations of this model are lack of dialysis and lack of host response

Moreover, such systems require proprietary technology and the use of expensive equipment, so this low-volume method

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