A kind of lactobacillus having the effect of preventing and treating diabetic encephalopathy and its isolation method and application

A technology of diabetic encephalopathy and lactobacillus, applied in the field of chemical medicine, can solve the problem of less diabetic encephalopathy, achieve the effect of reducing MDA content, improving SOD activity, and significant curative effect

Active Publication Date: 2022-06-21
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The important role of intestinal flora in systemic diseases has been paid more and more attention by researchers, but there are few studies on the prevention and treatment of diabetic encephalopathy through probiotics

Method used

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  • A kind of lactobacillus having the effect of preventing and treating diabetic encephalopathy and its isolation method and application
  • A kind of lactobacillus having the effect of preventing and treating diabetic encephalopathy and its isolation method and application
  • A kind of lactobacillus having the effect of preventing and treating diabetic encephalopathy and its isolation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Isolation and identification of Lactobacillus paracasei.

[0041] The specific method is as follows: take Qinghai old yogurt (product model 57338258454) as the isolated sample, dilute it, spread it on the sterile MRS medium with a coating rod, and carry out anaerobic culture at 37 ° C for 48 hours to obtain colonies; pick the morphology Regular colonies were streaked on sterile MRS medium for 5 times to isolate and purify to obtain a single pure colony, which was confirmed to be Lactobacillus by microscopic examination. Encephalopathy efficacy of Lactobacillus. The formula of MRS medium is: casein peptone 10.0g / L, beef extract 10.0g / L, yeast extract 5.0g / L, glucose 5.0g / L, sodium acetate 5.0g / L, diamine citrate 2.0 g / L, Tween-80 1.0g / L, dipotassium hydrogen phosphate 2.0g / L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate heptahydrate 0.05g / L, calcium carbonate 20.0g / L, agar 20.0g / L. pH6.8, sterilized at 121°C for 20min.

[0042] (1) Carry out 16S rDNA mol...

Embodiment 2

[0050] Preparation of Lactobacillus paracasei powder.

[0051] The specific method is as follows: pick a single colony of Lactobacillus paracasei LC86, inoculate it in 50 mL MRS medium, pH 6.8, and anaerobic culture at 37 °C for 24 h to obtain seed liquid; inoculate the seed liquid with 2% of the inoculum to new MRS In the culture medium, anaerobic culture at 37°C for 24 hours to obtain a fermentation broth; centrifuge the fermentation broth at 12000 rpm for 10 minutes, remove the fermentation supernatant, and separate the bacteria; freeze-dry the bacteria to obtain Lactobacillus paracasei bacteria pink.

Embodiment 3

[0053] Animal experimental research on prevention and treatment of diabetic encephalopathy.

[0054] 1. Experimental materials

[0055] 1. Lactobacillus paracasei LC86 powder, viable count 1×10 8 CFU / g, the preparation refers to Example 2.

[0056] 2. STZ solution: Weigh 100 mg of streptozotocin, add 5 mL of citric acid buffer, dissolve streptozotocin in an ice bath, and use it within 30 minutes.

[0057] 3. Experimental animals: Wistar rats, male, 12 weeks old, weighing 230-250 g.

[0058] 2. Preparation of the rat model of diabetic encephalopathy

[0059] The rats were fed adaptively for one week, and the rats with similar learning and memory abilities were screened out by using the Y-maze to establish the diabetic encephalopathy model. Wistar male rats were fasted for 12 hours, and STZ solution was injected intraperitoneally at 60 mg / Kg. According to the habitual modeling method of diabetic rats, the fasting blood glucose value of each rat was detected by two tail bloo...

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Abstract

The invention relates to a lactobacillus capable of preventing and treating diabetic encephalopathy, an isolation method and application thereof. The lactobacillus is named as Lactobacillus paracasei (Lactobacillus paracasei) LC86 strain, and the preservation unit is the General Microbiology Center of China Microbiological Culture Collection Management Committee. The preservation time is December 2013, and the preservation number is CGMCC 1.12731. The address is: Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District. It can significantly improve the elevated nitric oxide content caused by diabetic encephalopathy, increase the activity of antioxidant enzymes in brain tissue, and improve the ability of learning and memory. It is applied to the medicine for preventing and treating diabetic encephalopathy, has no toxic and side effects, and can be used together with other nutrients for the treatment of clinical patients.

Description

technical field [0001] The invention belongs to the field of chemical medicine, and in particular relates to a lactobacillus and a separation method and application thereof. In particular, it relates to a Lactobacillus with the effect of preventing and treating diabetic encephalopathy and its separation method and application. Background technique [0002] Diabetes has a high incidence, many complications, high mortality and disability rates, most of which are type Ⅱ diabetes. Long-term elevated blood sugar in diabetic patients without control will lead to various chronic complications, such as coronary heart disease, hypertension, myocardial infarction, stroke, Alzheimer's, Parkinson's, kidney disease, retinopathy, etc. Diabetic complications have become the main cause of death and disability in diabetic patients, including diabetic encephalopathy, which obviously has a serious impact on the quality of life of diabetic patients. [0003] Diabetic encephalopathy is easily ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A61K35/747A61P3/10A61P25/00C12R1/225
CPCC12N1/20C12N1/02A61K35/747A61P3/10A61P25/00C12R2001/225C12N1/205
Inventor 方曙光郝沛妍朱建国陈珂可于惠程文蒋大成
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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