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A strain am101 capable of degrading various β-lactam antibiotics and its application

A technology of lactams and antibiotics

Active Publication Date: 2021-01-19
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reported strains that can rapidly and efficiently degrade β-lactam antibiotics, especially the strains that degrade multiple β-lactam antibiotics at the same time have not been reported.

Method used

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  • A strain am101 capable of degrading various β-lactam antibiotics and its application
  • A strain am101 capable of degrading various β-lactam antibiotics and its application
  • A strain am101 capable of degrading various β-lactam antibiotics and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Screening of β-lactam antibiotic-degrading strain Am101

[0021] 1. Material preparation

[0022] The source of bacteria samples: fresh pig manure and chicken manure from a livestock and poultry breeding enterprise.

[0023] Antibiotics: penicillin potassium, amoxicillin, cefuroxime sodium, ceftiofur sodium.

[0024] Beef extract peptone medium: 5.0g beef extract, 10.0g peptone, 5.0g NaCl, distilled water, pH7.4-7.6, sterilized at 121°C for 15min. Add 10g-15g of agar to the solid medium.

[0025] Medium No. Ⅱ: beef extract 1.5g, peptone 6.0g, yeast extract powder 6.0g, glucose 1.0g, water 1L, sterilized at 115°C for 20min. Add 10g-15g of agar to the solid medium.

[0026] Inorganic salt medium: (NH 4 ) 2 NO 4 2.0g, K 2 HPO 4 0.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl0.2g, CaCl 2 0.1g, MnSO 4 ·H 2 O 0.01g, FeSO 4 ·7H 2 O 0.01g, distilled water 1L, sterilized at 121°C for 15-20min. Add 10g-15g of agar to the solid medium.

[0027] Martin's Me...

Embodiment 2

[0046] Growth and degradation characteristics of strain Am101

[0047] 1. Growth characteristics of strain Am101

[0048] The strain Am101 was inoculated in different solid media such as beef extract peptone medium, Martin's medium, potato medium, and inorganic salt medium. Potassium, amoxicillin, cefuroxime sodium, and ceftiofur sodium can also grow in the beef extract peptone medium, and the cultivation is easy.

[0049] The single factor test was used to study the suitable culture conditions of the strain Am101, and the setting experiment included temperature 25°C-55°C, pH value 5-9, concentration of β-lactam antibiotics 50mg / L-150mg / L, and culture time 3-7 Today, it was found that the strain Am101 can grow under the conditions of 25°C-55°C, pH value 5-9, and β-lactam antibiotic concentration of 50mg / L-150mg / L.

[0050] Inoculate the strain Am101 in the beef extract peptone liquid medium, place it at 28--30°C, shake it at 150-180r / min, take samples regularly, measure the ...

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Abstract

The invention discloses a strain of Bacillus tequila Am101 capable of degrading various β-lactam antibiotics and its application. The strain was deposited in the General Microorganism Center of China Committee for Microorganism Culture Collection on November 18, 2019, with the preservation number CGMCC NO.18965. The bacterial strain Am101 of the present invention can simultaneously degrade multiple β-lactam antibiotics, and can efficiently degrade penicillin potassium, amoxicillin, cefuroxime sodium and ceftiofur sodium. Strain Am101 can be applied to the degradation treatment of residual β-lactam antibiotics in livestock and poultry manure, and can also be used to restore water and soil polluted by β-lactam antibiotics, with broad application prospects.

Description

technical field [0001] The invention relates to a bacterial strain Bacillus tequilensis (Bacillus tequilensis) Am101 capable of degrading multiple β-lactam antibiotics, belonging to the technical field of microorganisms. Background technique [0002] β-lactam antibiotics refer to a large class of antibiotics containing a β-lactam ring in their molecular structure, including penicillins with 6-APA as their nucleus and cephalosporins with 7-ACA as their nucleus. It is widely used in the fields of animal husbandry, fishery and clinical medicine because of its broad antibacterial spectrum, strong antibacterial effect and low price. It is the most widely used class of existing antibiotics. [0003] During the fermentation and production of β-lactam antibiotics, a large amount of bacterial residue and waste water will be produced. If they are not properly treated and discharged into the environment, they may cause residues in soil, water and food; during the use of antibiotics, on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/07C02F101/38A62D101/28
CPCA62D3/02A62D2101/28B09C1/10C02F3/34C02F2101/38C12N1/205C12R2001/07
Inventor 李艳菊王梓诺顾金刚杨正礼张爱萍
Owner BEIJING INSTITUTE OF TECHNOLOGYGY