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Method for measuring content of spermidine in safflower medicinal material and method for enriching spermidine

A determination method and spermidine technology, applied in the field of detection and analysis, can solve the problem of not providing a spermidine compound safflower drug detection method and the like

Active Publication Date: 2020-02-14
YAAN THREE NINE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The prior art does not provide a reliable detection method for safflower drugs with less spermidine compound content

Method used

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  • Method for measuring content of spermidine in safflower medicinal material and method for enriching spermidine
  • Method for measuring content of spermidine in safflower medicinal material and method for enriching spermidine
  • Method for measuring content of spermidine in safflower medicinal material and method for enriching spermidine

Examples

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Embodiment 1

[0068] This example provides a method for enriching spermidine in safflower injection and a method for determining spermidine in safflower injection.

[0069] The enrichment method of spermidine in safflower injection comprises the following steps:

[0070] Take 50 mL of safflower injection, and filter it with a nylon 66 microporous filter membrane with a pore size of 0.2 μm; after filtering, cut the filter membrane into pieces, add 30 mL methanol for ultrasonic extraction, and filter through filter paper to get the filtrate.

[0071] The assay method of spermidine in safflower injection comprises the following steps:

[0072] The method for enriching spermidine in safflower injection provided in this example was used to enrich spermidine in safflower injection.

[0073] Take 20mL of the filtrate, blow it with nitrogen until the solution is less than 2mL, transfer it to a 2mL volumetric flask, adjust the volume to the mark with methanol, shake well, filter it with a microporo...

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Abstract

The application relates to the field of detection and analysis, and specifically relates to a method for measuring a content of spermidine in a safflower medicinal material and a method for enrichingspermidine. The method for measuring the content of the spermidine in the safflower medicinal material includes the following steps: using nylon 66 to adsorb the spermidine in a safflower medicinal material solution to obtain an enriched substance; dissolving the enriched substance by using a solvent and then performing filtering; and measuring the spermidine in a filtrate by using high performance liquid chromatography. The solvent includes a methanol aqueous solution having a volume fraction of 70% to 100% or an ethanol aqueous solution having a volume fraction of 70% to 100%. For the safflower medicinal material solution with a relatively low spermidine content (such as a safflower injection of about 0.15 [mu]g / mL), the nylon 66 has a function of enrichment and concentration, provides agood test condition for high performance liquid chromatography measurement, and provides a test method for a safflower-containing medicinal material with low concentration of spermidine.

Description

technical field [0001] The application relates to the field of detection and analysis, in particular to a method for determining the content of spermidine in safflower medicinal materials and a method for enriching spermidine. Background technique [0002] Safflower is a plant of the genus Safflower in the Compositae family. It can promote blood circulation, relieve menstruation, relieve pain, lower blood pressure and lower blood lipids. Safflower contains spermidine compounds, which refer to compounds with H 2 N(CH 2 ) 3 NH(CH 2 ) 4 NH 2 Structural units of polyamine compounds. [0003] The prior art does not provide a reliable detection method for safflower drugs with less spermidine compound content. Contents of the invention [0004] The purpose of the embodiments of the present application is to provide a method for determining the content of spermidine in safflower medicinal materials and a method for enriching spermidine, which aims to provide a safflower dru...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/08G01N30/14
CPCG01N30/02G01N30/08G01N30/14G01N2030/146
Inventor 董礼张翅严敏嘉王丽婷吴建国
Owner YAAN THREE NINE PHARMA
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