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Cell transport protection liquid, preparation method and applications thereof

A protection solution and cell technology, applied in the biological field, can solve the problems of unsafe research experiments, inability to directly observe the state, and affect the survival rate of cells, etc., to maintain a sterile growth environment, suitable for large-scale production applications, and good antibacterial sterilization The effect of action

Active Publication Date: 2020-02-21
KYBIOSTEM CO LTD
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Problems solved by technology

[0005] At present, in order to ensure the activity of mesenchymal stem cells during the transportation process, the cells are usually frozen and then transported with liquid nitrogen or dry ice. The transportation conditions are very harsh. It is necessary to ensure that the whole transportation is in a low temperature state, and the cost is high.
And if the cells are repeatedly frozen and thawed during transportation, the cell viability will be affected
In addition, the state of the received cells cannot be directly observed, and there is no guarantee for subsequent research experiments

Method used

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  • Cell transport protection liquid, preparation method and applications thereof
  • Cell transport protection liquid, preparation method and applications thereof
  • Cell transport protection liquid, preparation method and applications thereof

Examples

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preparation example Construction

[0066] The preparation method of the cell transport protection solution provided by the present invention has simple process and convenient operation, as long as the protective agent, basal medium, albumin, antibiotic and water are dissolved and mixed uniformly, the cell transport protection solution can be obtained. The preparation method does not require expensive instruments, can effectively save costs, and is suitable for large-scale production applications.

[0067] In some specific embodiments, taking the configuration of 1L cell transport protection solution as an example, under sterile conditions, take 0.1-0.3L basal medium plus 0.01-0.03L albumin, then add 2-15g of protective agent and 0.2g of antibiotic -1.5g, stir to dissolve, add sterile deionized water to a constant volume of 1L, stir evenly to obtain a cell transport protection solution.

[0068] In addition, the present invention also provides the application of the above-mentioned cell transport protection solu...

Embodiment 1

[0071] This embodiment provides a cell transport protection solution, each 1L of the cell transport protection solution includes: hydroxypropyl cellulose 5g, MEM 0.2L, fetal bovine serum 0.015L, penicillin 0.2g, streptomycin 0.2g and other amount of water.

Embodiment 2

[0073] This example provides a cell transport protection solution, each 1L of the cell transport protection solution includes: 10 g of low-viscosity carboxymethyl cellulose sodium salt, 0.15 L of MEM, 0.025 L of fetal bovine serum, 0.5 g of penicillin, streptomycin 0.5 g and the balance water.

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Abstract

The invention provides a cell transport protection liquid, a preparation method and applications thereof, and relates to the technical field of biology, wherein the cell transport protection liquid comprises a protection agent, a basal culture medium, albumin, antibiotics and water, wherein the protection agent comprises cellulose ether, cyclodextrin, polymeric alcohol or poloxamer 188, and the substances are used as the protection agent, so that the cost is low, the cell growth speed can be reduced, and the influence on the cell activity due to over-dense cell growth can be avoided. Accordingto the invention, the protection agent can increase the viscosity of the cell transport protection liquid, and effectively avoids the cell physical damage caused by vibration and shaking in a transportation process; the basal culture medium is rich in cell metabolism essential components, and albumin can provide nutrition for cells, so that the activity of the cells is effectively maintained; andthe antibiotics can provide good bacteriostasis and sterilization effect, and maintain the sterile growth environment of cells.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cell transport protection solution and its preparation method and application. Background technique [0002] With the development of medicine, in the process of clinical application and scientific research practice, people's demand for preservation of isolated organs, tissues and cells is constantly increasing, and the standards are constantly improving. In the process of preservation and transportation of some organs or tissues to be transplanted , but also put forward higher requirements for the medium of cell survival. It is necessary to ensure the viability of organs, tissues or cells, but also to retain their original biological activity to the greatest extent, and at the same time prevent them from being polluted by bacteria, etc., and preventing their death. The attributes of various organs, tissues or cells are different, and their preservation media also have different re...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0226
Inventor 焦晓敏杜宏武
Owner KYBIOSTEM CO LTD
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