Dendrobium officinale refined polysaccharide extract and application thereof

A technique for extracting polysaccharides and Dendrobium candidum, applied in the field of refined polysaccharide extracts from Dendrobium candidum, can solve the problems of few documents and patents, no related products, reports, etc., and achieve the effect of high active ingredients, good product color and simple process

Inactive Publication Date: 2020-02-21
韶关市丹霞铁皮石斛研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the medicinal use of Dendrobium officinale polysaccharides in colon cancer is still blank, there are no related products, and there are few relevant literature and patent reports

Method used

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  • Dendrobium officinale refined polysaccharide extract and application thereof
  • Dendrobium officinale refined polysaccharide extract and application thereof
  • Dendrobium officinale refined polysaccharide extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of dendrobium officinale refined polysaccharide extract, comprising the steps of:

[0030] (1) Weigh 50 g of Dendrobium officinale powder, then add 900 mL of petroleum ether to the Dendrobium officinale leaf powder, reflux extraction for 1 hour, filter, discard the filtrate, and obtain filter residue A;

[0031] (2) Add 900mL 80% ethanol solution to the filter residue A, reflux for extraction for 1h, filter, discard the filtrate, then add 1250mL water to the filter residue, reflux for 1h, filter, collect the filtrate, add water to the filter residue and repeat the extraction twice, Combine the filtrates and concentrate under reduced pressure at 60°C to obtain 390 mL of concentrated solution;

[0032] (3) Add 130mL sevage (chloroform: n-butanol = 4:1) reagent to the concentrated solution to deproteinize, shake well, let stand, discard the lower chloroform layer and the middle white protein layer, collect the upper polysaccharide solution, and repeat ...

Embodiment 2

[0036] A preparation method of dendrobium officinale refined polysaccharide extract, comprising the steps of:

[0037] (1) Weigh 50 g of Dendrobium officinale powder, then add 1250 mL of petroleum ether to the Dendrobium officinale leaf powder, reflux extraction for 2 hours, filter, discard the filtrate, and obtain filter residue A;

[0038] (2) Add 1000mL of 80% ethanol solution to the filter residue A, reflux for extraction for 2h, filter, discard the filtrate, then add 1250mL of water to the filter residue, reflux for 2h, filter, collect the filtrate, add water to the filter residue and repeat the extraction twice, Combine the filtrates and concentrate under reduced pressure at 70°C to obtain 400 mL of concentrated solution;

[0039] (3) Add 100mL sevage (chloroform: n-butanol = 4:1) reagent to the concentrated solution to deproteinize, shake well, let stand, discard the lower chloroform layer and the middle white protein layer, collect the upper polysaccharide solution, an...

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Abstract

The invention discloses a Dendrobium officinale refined polysaccharide extract and an application thereof. A preparation method of the polysaccharide comprises the following steps: adding petroleum ether into Dendrobium officinale powder, carrying out reflux extraction, filtering the obtained mixture, and discarding the obtained filtrate to obtain a filter residue A; adding a 80% ethanol solutioninto the filter residue A, carrying out reflux extraction, filtering the obtained solution, discarding the obtained filtrate, adding water into the obtained filter residue, carrying out reflux extraction, filtering the obtained solution, collecting the obtained filtrate, mixing the obtained filtrates, and carrying out reduced pressure concentration to obtain a concentrate; adding a sevage reagentinto the concentrate to perform deproteinization in order to obtain a deproteinized crude polysaccharide concentrate; adding anhydrous ethanol to the crude polysaccharide concentrate, standing the obtained system, centrifuging the system, and collecting the obtained precipitate; dissolving the precipitate in distilled water, and performing vacuum freeze drying to obtain crude polysaccharide; and separating and purifying the crude polysaccharide by sequentially adopting anion exchange resin and Sephadex resin, and carrying out vacuum freeze drying to obtain the Dendrobium officinale refined polysaccharide. The polysaccharide extract obtained in the invention is applied to the preparation of a medicine for treating colon cancer.

Description

technical field [0001] The invention relates to the technical field of dendrobium candidum, in particular to a refined polysaccharide extract of dendrobium candidum and an application thereof. Background technique [0002] Dendrobium officinale kimura et Migo is the dry stem of the orchid plant (Dendrobium officinale kimura et Migo). It is a traditional rare and rare Chinese medicinal material and a good health product for both medicine and food. It has the functions of treating cancer, lowering blood sugar, enhancing immunity, anti-oxidation, and lowering blood lipids. And many other effects, and the toxic and side effects are small. [0003] Among them, Dendrobium officinale polysaccharide has the effect of improving immunity and anti-tumor; it can improve hematopoiesis and blood coagulation function, and it also has certain effects on lowering blood sugar; it has effects on the treatment of central nervous system, liver, kidney, and gastrointestinal diseases; it has effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 陶盛昌魏刚张小凤
Owner 韶关市丹霞铁皮石斛研究院
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