Dendrobium officinale refined polysaccharide extract and application thereof
A technique for extracting polysaccharides and Dendrobium candidum, applied in the field of refined polysaccharide extracts from Dendrobium candidum, can solve the problems of few documents and patents, no related products, reports, etc., and achieve the effect of high active ingredients, good product color and simple process
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Embodiment 1
[0029] A preparation method of dendrobium officinale refined polysaccharide extract, comprising the steps of:
[0030] (1) Weigh 50 g of Dendrobium officinale powder, then add 900 mL of petroleum ether to the Dendrobium officinale leaf powder, reflux extraction for 1 hour, filter, discard the filtrate, and obtain filter residue A;
[0031] (2) Add 900mL 80% ethanol solution to the filter residue A, reflux for extraction for 1h, filter, discard the filtrate, then add 1250mL water to the filter residue, reflux for 1h, filter, collect the filtrate, add water to the filter residue and repeat the extraction twice, Combine the filtrates and concentrate under reduced pressure at 60°C to obtain 390 mL of concentrated solution;
[0032] (3) Add 130mL sevage (chloroform: n-butanol = 4:1) reagent to the concentrated solution to deproteinize, shake well, let stand, discard the lower chloroform layer and the middle white protein layer, collect the upper polysaccharide solution, and repeat ...
Embodiment 2
[0036] A preparation method of dendrobium officinale refined polysaccharide extract, comprising the steps of:
[0037] (1) Weigh 50 g of Dendrobium officinale powder, then add 1250 mL of petroleum ether to the Dendrobium officinale leaf powder, reflux extraction for 2 hours, filter, discard the filtrate, and obtain filter residue A;
[0038] (2) Add 1000mL of 80% ethanol solution to the filter residue A, reflux for extraction for 2h, filter, discard the filtrate, then add 1250mL of water to the filter residue, reflux for 2h, filter, collect the filtrate, add water to the filter residue and repeat the extraction twice, Combine the filtrates and concentrate under reduced pressure at 70°C to obtain 400 mL of concentrated solution;
[0039] (3) Add 100mL sevage (chloroform: n-butanol = 4:1) reagent to the concentrated solution to deproteinize, shake well, let stand, discard the lower chloroform layer and the middle white protein layer, collect the upper polysaccharide solution, an...
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