A preparation method of multi-dimensional composite cross-linked hyaluronic acid gel and its products and applications
A technology of hyaluronic acid and compound cross-linking, applied in the fields of medical cosmetology and biomedicine, can solve the problems that the cross-linking uniformity of the reaction system cannot be guaranteed, the anti-degradation ability of products varies greatly, and the reaction process cannot be consistent, etc., so as to reduce degradation. and the possibility of side reactions, the uniform mixing of the reaction system, the effect of shortened time
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Embodiment 1
[0043] Embodiment 1 investigates high temperature crosslinking temperature
[0044] Follow the steps below to prepare multidimensional composite cross-linked hyaluronic acid gel:
[0045] (1) Dissolve 0.032g of 1,4-butanediol diglycidyl ether in 20g of sodium hydroxide solution with a pH value of 9 to 13, mix well and add 4g of sodium hyaluronate with a molecular weight of 2 million Daltons (Based on pure product), mix well until the gel is clear and transparent, control the reaction temperature: A 35°C, B 50°C and C 65°C, respectively react for 2 hours to obtain gel I.
[0046] (2) Dissolve 0.048g of 1,4-butanediol diglycidyl ether in 16g of glacial acetic acid solution with a pH value of 3 to 5, mix well and add 4g of sodium hyaluronate with a molecular weight of 2 million Daltons ( Based on the pure product), mix well until the gel is clear and transparent, control the reaction temperature: A 35°C, B 55°C and C 65°C, and react for 2 hours respectively to obtain Gel II.
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Embodiment 2
[0051] Embodiment 2 investigates high temperature crosslinking time
[0052] Follow the steps below to prepare multidimensional composite cross-linked hyaluronic acid gel:
[0053] (1) Dissolve 0.032g of 1,4-butanediol diglycidyl ether in 20g of sodium hydroxide solution with a pH value of 9 to 13, mix well and add 4g of sodium hyaluronate with a molecular weight of 2 million Daltons (Based on pure product), mix well until the gel is clear and transparent, control the reaction temperature at 50°C, and react separately: A for 0.5h, B for 2h and C for 12h to obtain Gel I.
[0054] (2) Dissolve 0.048g of 1,4-butanediol diglycidyl ether in 16g of glacial acetic acid solution with a pH value of 3 to 5, mix well and add 4g of sodium hyaluronate with a molecular weight of 2 million Daltons ( Calculated as a pure product), mix well until the gel is clear and transparent, control the reaction temperature at 55°C, and react separately: A0.5h, B 2h and C12h to obtain gel II.
[0055] (...
Embodiment 3
[0059] Embodiment 3 investigates cross-linking agent consumption
[0060] Follow the steps below to prepare multidimensional composite cross-linked hyaluronic acid gel:
[0061] (1) Dissolve 1,4-butanediol diglycidyl ether: 0.006g of A, 0.016g of B, and 0.2g of C in 10g of sodium hydroxide solution with a pH value of 9 to 13, mix well and add 2g of molecular weight Sodium hyaluronate (calculated as pure product) is 2 million daltons, mixed evenly until the gel is clear and transparent, the reaction temperature is controlled at 50°C, and reacted for 2 hours to obtain gel I.
[0062] (2) Dissolve 1,4-butanediol diglycidyl ether: A 0.006g, B 0.028g, C 0.2g in 8g of glacial acetic acid solution with a pH value of 3~5, mix well and add 2g of Sodium hyaluronate (calculated as a pure product) of 2 million daltons was mixed evenly until the gel was clear and transparent, the reaction temperature was controlled at 55°C, and the reaction was carried out for 2 hours to obtain gel II.
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