Application of gene SOX30 in treatment of non-obstructive azoospermia

A SOX30, azoospermia technology, applied in the field of molecular biology, can solve the problem of poor treatment effect of NOA patients, to avoid blind abuse and improve accuracy

Pending Publication Date: 2020-02-28
ARMY MEDICAL UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] This application provides the application of gene SOX30 in the treatment of non-obstructive azoospermia, in order to solve the problem that the existing assisted reproductive technology has poor treatment effect on most NOA patients

Method used

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  • Application of gene SOX30 in treatment of non-obstructive azoospermia
  • Application of gene SOX30 in treatment of non-obstructive azoospermia
  • Application of gene SOX30 in treatment of non-obstructive azoospermia

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Select 3 cases of testicular tissue puncture from OA patients and 3 cases of testicular tissue from NOA patients whose indicators are close to normal human testicular morphology and pathology, and perform methylation microarray and expression profiling microarray respectively to detect the methylation and expression of SOX30 in testicular puncture of NOA patients. changes in the organization. Such as figure 1 The results showed that compared with the methylation and expression of SOX30 in testicular puncture tissues of OA patients, the SOX30 gene promoter was significantly hypermethylated in NOA puncture testicular tissues, and its expression was significantly reduced or silenced in NOA puncture testicular tissues. That is to say, the hypermethylation of the SOX30 gene promoter in NOA has resulted in the expression silencing of the SOX30 gene in NOA. Therefore, it can be determined that the methylation inactivation of the SOX30 gene is involved in the formation and deve...

Embodiment 2

[0034] In different genotype mouse testes, the chromosome diffusion experiment was performed by fluorescently staining SCP3, and the changes in cell morphology and cell number at each stage of germ cell meiosis were analyzed, such as Figure 6A and Figure 6B The results showed that SOX30 - / - Germ cell meiosis in mouse testis is mainly arrested at the spermatocyte stage of zygotene. Further transcriptome sequencing was performed on the testes of different genotypes to analyze the mechanism of SOX30 deletion leading to germ cell meiosis arrest, such as Figure 7A The results showed that the expression of RA signaling pathway including key genes REC8, STRA8 and CYP26B1 was significantly affected. In the testes of SOX30 knockout mice, the expressions of REC8 and STRA8 were significantly decreased, while the expression of CYP26B1 was significantly increased; the different genes were analyzed by quantitative-PCR The expression changes of SOX30, REC8, STRA8 and CYP26B1 in the test...

Embodiment 3

[0037] In the constructed inducible gene knockout model, the adult SOX30 - / - Mice undergo estrogen-induced deletion of the knockout element to re-express SOX30, and analyze and compare the SOX30 that restores the expression of SOX30 - / - Mice (SOX30 loxp / loxp ) and testicular volume and pathological morphology of mice in the control group, such as Figure 9 The results showed that after restoring the expression of SOX30, the testicular damage of the mice was significantly improved, and the spermatogenic process of the mouse testis returned to normal after restoring the expression of SOX30, and a large number of sperm reappeared in the epididymis. Figure 10 The results showed that these recovered sperm can carry out normal conception ability and produce offspring, and the growth and fertility of these offspring individuals are normal, and no obvious negative effects are found.

[0038] Figure 11 The results showed that in SOX30 loxp / loxp When the expression changes of key ...

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Abstract

The invention discloses application of gene SOX30 in the treatment of non-obstructive azoospermia. The invention confirms the relation between the SOX30 and the non-obstructive azoospermia, so that the SOX30 can be used as the medicine, medicine target or target gene in gene therapy to apply to the treatment and relieving of the non-obstructive azoospermia and can provide a new strategy for the treatment and relieving of the non-obstructive azoospermia.

Description

technical field [0001] The application relates to the technical field of molecular biology, in particular to the application of the gene SOX30 in the treatment of non-obstructive azoospermia. Background technique [0002] As an important disease affecting human reproduction, infertility has become a serious medical health problem and a serious social problem worldwide, and has attracted widespread attention. The World Health Organization (WHO) predicts that infertility will become the third largest disease after tumors and cardiovascular diseases among diseases affecting human life and health. According to the latest statistics from WHO, there are about 80 million couples suffering from infertility in the world, of which male patients account for about 50%, and the proportion of infertility caused by males is increasing year by year. The etiology of male infertility is very complex, and the pathological mechanism is still unclear. Although some male infertility is caused b...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K38/17A61K45/00A61P15/08
CPCA61K48/005A61K38/1709A61K45/00A61P15/08
Inventor 韩飞刘晋祎曹佳尹俐姜晓张玺敖琳
Owner ARMY MEDICAL UNIV
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