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Isocarbophos monoclonal antibody and application thereof to colloidal gold test paper

A technology of isocarbophos monoclonal antibody, applied in the direction of measuring devices, instruments, analytical materials, etc., can solve the problems that it is difficult to meet the fast and online detection, the application limitation of ELISA method, and the inability to perform online detection, etc., so as to achieve easy promotion, Wide range of use, easy to promote the effect of application

Inactive Publication Date: 2020-02-28
SUZHOU KUAIJIEKANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Instrumental analysis methods include high performance liquid chromatography (High performance liquid chromatography, HPLC), gas chromatography (Gas chromatography, GC), thin layer chromatography (Thin layer chromatography, TLC), liquid-mass tandem method (HPLC-MS) Although these instrumental analysis methods can achieve high detection sensitivity, they require complex and expensive instruments and professional operators. In addition, the sample pretreatment is cumbersome and time-consuming, and the detection cost is high. The need for fast, online testing
ELISA (Enzyme Linked Immunosorbent Assay) method is a commonly used immunoassay method, but its operation steps are relatively many, the detection time is long, the detection result is not intuitive enough, and it cannot be detected online.
Therefore, the application of ELISA method is greatly limited.

Method used

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  • Isocarbophos monoclonal antibody and application thereof to colloidal gold test paper
  • Isocarbophos monoclonal antibody and application thereof to colloidal gold test paper
  • Isocarbophos monoclonal antibody and application thereof to colloidal gold test paper

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Embodiment

[0023] Embodiment: 1, the synthesis of isocarbophos hapten (O-methyl-O-(2-isopropoxycarbonylphenyl)-N-(3-carboxypropyl) phosphorothioate):

[0024] ① Synthesis of O-methyl-O-(2-isopropoxycarbonylphenyl) phosphorothioate amine. In the reaction bottle, add 2.47g (15mmol) O-methylphosphoryl thiodichloride, 4.27g (30mmol) finely ground K 2 CO 3 And 0.8ml acetonitrile, stir, be cooled to below 10 ℃, dropwise add the acetonitrile (0.4ml) solution containing 1.8g (10mmol) isopropyl salicylate, continue stirring at room temperature for 30 minutes, evaporate the solvent under reduced pressure, column After separation by chromatography (silicagel, 2:1 hexanes-benzene), 2.85 g of product (a) was obtained as a colorless liquid (92.5% yield).

[0025] ② Synthesis of O-methyl-O-(2-isopropoxycarbonylphenyl)-N-(3-carboxypropyl) phosphorothioate. Add 0.65g (2.1mmol) O-methyl-O-(2-isopropoxycarbonylphenyl) thiophosphoryl chloride (a) and 3ml methanol in the reaction flask, cool in an ice-wat...

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Abstract

The invention relates to an isocarbophos monoclonal antibody and application thereof to colloidal gold test paper. An amino acid sequence in a heavy-chain variable region of the antibody is shown as SEQ ID NO:1, and an amino acid sequence in a light-chain variable region of the antibody is shown as SEQ ID NO:2. The colloidal gold test paper comprises a substrate, a sample pad, a gold label combination pad, a cellulose membrane and a water absorption pad, and the sample pad, the gold label combination pad, the cellulose membrane and the water absorption pad are arranged on the substrate sequentially. The isocarbophos monoclonal antibody is adsorbed in the gold label combination pad, the cellulose membrane is provided with an invisible detection line and an invisible control line, the invisible detection line and the invisible control line are parallel, the invisible detection line is printed with isocarbophos hapten coupled carrier protein solution, and the invisible control line is printed with a rabbit anti-rat IgG antibody. Results can be judged within several minutes according to visible strips without professionals and any auxiliary instruments, simplicity and convenience in operation, quickness, direct and accurate results and low cost are realized, and testing can be performed outdoors.

Description

technical field [0001] The invention relates to the technical field of pesticide residue immune analysis, in particular to an isocarbophos monoclonal antibody and its application in colloidal gold detection test paper. Background technique [0002] Isocarbophos (Isocarbophos) is a broad-spectrum organophosphorus insecticide used to control pests in stored grains and various foliage crops, and is also used to control mosquito adults, flies, aquatic larvae and sanitary pests. Its mechanism of action is to inhibit the activity of acetylcholinesterase. Acetylcholine is a neurotransmitter, and excess acetylcholine overstimulates acetylcholine receptors, leading to the death of target organisms. However, some creatures that were not the intended target were also poisoned, including humans, fish and other creatures. [0003] At present, the detection methods for pesticide residues are mainly instrumental analysis methods and enzyme-linked immunoassay methods (ELISA). Instrument...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/44G01N33/543G01N33/577
CPCC07K16/44G01N33/543G01N33/577G01N2430/10
Inventor 盛相国
Owner SUZHOU KUAIJIEKANG BIOTECH CO LTD
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