Analysis method for evaluating pharmacokinetic matrix effect of Chinese patent medicine
A technology of matrix effect and analysis method, which is applied in the analysis field of evaluating the pharmacokinetic matrix effect of Chinese patent medicine, and can solve the problems that no one has proposed to systematically investigate the matrix effect of Chinese medicine pharmacokinetics
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Embodiment 1
[0099]SD rats were selected as experimental animals, and SD rats were SPF grade, male, weighing 230-270 g.
[0100] Xuebijing is selected as the Chinese patent medicine, which is composed of safflower, red peony root, salvia miltiorrhiza, Chuanxiong and angelica. A large number of clinical and basic studies have proved that Xuebijing is an effective Chinese patent medicine for the treatment of sepsis, and its curative effect mainly depends on the key components that can be effectively used by the body. Safflower is a monarch drug, and its main active ingredients are flavonoids. The content of hydroxy A in flavonoids (0.2–0.5mg / mL) is much higher than other flavonoids, and it is one of the quality control components of Xuebijing. Previous pharmacokinetic studies on Xuebijing showed that after administration of Xuebijing, hydroxy A was significantly exposed in vivo compared with other Xuebijing substances and was mainly eliminated from the body through urinary excretion in the ...
Embodiment 2
[0124] Prepare the standard solution whose concentration range of hydroxysafflower yellow A is 15.6-8000nmol / L, carry out LC / MS / MS detection, take the chromatographic peak area as the ordinate (Y axis), the quality of its corresponding hydroxysafflower yellow A The concentration is the abscissa (X-axis), and the regression analysis is carried out to obtain the regression equation and its correlation coefficient, as shown in Table 1. It can be seen from Table 1 that the linear relationship of the above regression equation is good when the sample is injected within the corresponding concentration range, and the correlation coefficient r>0.99.
[0125] Table 1
[0126]
Embodiment 3
[0128] According to Example 1, the plasma sample a of Xuebijing 10 mL / kg was administered to rats via tail vein injection, and the plasma concentration data of hydroxy A in the body are shown in Table 2. Table 3 shows the plasma concentration of hydroxy A in rats after tail vein injection of 4.2 mg / kg hydroxy A solution in plasma sample b.
[0129] With the blood drug concentration data of hydroxy A in the rat body in table 2 and table 3, draw the hydroxy A mean blood drug concentration-time curve, obtain figure 1 , figure 2 ,in, figure 1 It is the average blood drug concentration-time curve of hydroxy A in rats after administration of Xuebijing; figure 2 It is the average plasma concentration-time curve of hydroxy A in rats after administration of hydroxy A solution.
[0130] The blood concentration data of hydroxy A in rats in Table 2 and Table 3 were calculated using the pharmacokinetic software Kinetica5.0 to obtain plasma pharmacokinetic parameter data Tables 4 and 5...
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